Abstract
We report the development of a publicly accessible, curated nucleotide sequence database of hypocrealean entomopathogenic fungi. The goal is to provide a platform for users to easily access sequence data from taxonomic reference strains. The database can be used to accurately identify unknown entomopathogenic fungi based on sequence data for a variety of phylogenetically informative loci. The database provides full multi-locus sequence alignment capabilities. The initial release contains data compiled for 525 strains covering the phylogenetic diversity of three important entomopathogenic families: Clavicipitaceae, Cordycipitaceae, and Ophiocordycipitaceae. Furthermore, Entomopathogen ID can be expanded to other fungal clades of insect pathogens, as sequence data becomes available. The database will allow isolate characterisation and evolutionary analyses. We contend that this freely available, web-accessible database will facilitate the broader community to accurately identify fungal entomopathogens, which will allow users to communicate research results more effectively.
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Acknowledgements
The authors would like to thank Heather Walker for data entry assistance. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the U.S. Department of Agriculture. The mention of firm names or trade products does not imply that they are endorsed or recommended by the USDA over other firms or similar products not mentioned. USDA is an equal opportunity provider and employer.
Funding
This project was funded by the U.S. Department of Agriculture, Agriculture Research Service, Project No. 5010-22410-017-00-D.
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Dunlap, C.A., Ramirez, J.L., Mascarin, G.M. et al. Entomopathogen ID: a curated sequence resource for entomopathogenic fungi. Antonie van Leeuwenhoek 111, 897–904 (2018). https://doi.org/10.1007/s10482-017-0988-2
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DOI: https://doi.org/10.1007/s10482-017-0988-2