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Isolation and characterization of a novel marine Bacteroidetes as Algitalea ulvae gen. nov., sp. nov., isolated from the green alga Ulva pertusa

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Abstract

A polyphasic taxonomic investigation was performed on a bacterial strain, 38-Ka-2T, which was isolated from the green alga Ulva pertusa Kjellman (Chlorophyta) in Hokkaido, Japan. The bacterial cells were observed to be golden-yellow coloured, Gram-negative, strictly aerobic, non-spore-forming, non-motile and rod-shaped. Phylogenetic analyses based on the 16S rRNA gene sequence indicated that the new strain is a member of the family Flavobacteriaceae within the phylum Bacteroidetes and that it shows high sequence similarity (94.8 %) to Aquimarina addita JC2680T. The genomic DNA G+C content of strain 38-Ka-2T was determined to be 36 mol%; MK-6 was identified as the major menaquinone; and the presence of iso-C15:0, C18:0 and iso-C17:0 3-OH as the major (>10 %) cellular fatty acids. The polar lipid profile was found to consist of phosphatidylethanolamine, an unidentified glycolipid and two unidentified lipids. From the distinct phylogenetic position and combination of genotypic and phenotypic characteristics, strain 38-Ka-2T is considered to represent a novel genus for which the name Algitalea ulvae gen. nov., sp. nov. is proposed. The type strain of A. ulvae is 38-Ka-2T (=KCTC 32994T = NBRC 110017T).

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Acknowledgments

This research was supported by the Bisa Research Grant of Keimyung University in 2015.

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The authors declare that they have no conflict of interest.

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Author notes

  1. Kyoko Adachi

    Present address: Basic Research Laboratories, Nippon Steel & Sumikin Chemical Co., Ltd., 1-Tsukiji, Kisarazu, Chiba, 292-0835, Japan

Authors and Affiliations

  1. College of Pharmacy, Keimyung University, 1095 Dalgubeoldaero, Dalseo-Gu, Daegu, 704-701, Republic of Korea

    Jaewoo Yoon

  2. Marine Biotechnology Institute, 3-75-1 Heita, Kamaishi, Iwate, 026-0001, Japan

    Kyoko Adachi

  3. Marine Biosciences Kamaishi Research Laboratory, Kitasato University, 160-4, Utou, Okirai, Sanriku-cho, Ofunato, Iwate, 022-0101, Japan

    Hiroaki Kasai

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  1. Jaewoo Yoon
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Correspondence to Jaewoo Yoon.

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Supplementary Fig. 1

Thin-layer chromatograms showing the total polar lipid composition of 38-Ka-2T. Total polar lipids were detected by spraying the plate with molybdophosphoric acid, molybdenun blue, α-naphthol and ninhydrin. PE-phosphatidylenthanolamine, GL-unidentified glycolipid, L-unidentified lipid. Supplementary material 1 (PPTX 701 kb)

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Yoon, J., Adachi, K. & Kasai, H. Isolation and characterization of a novel marine Bacteroidetes as Algitalea ulvae gen. nov., sp. nov., isolated from the green alga Ulva pertusa . Antonie van Leeuwenhoek 108, 505–513 (2015). https://doi.org/10.1007/s10482-015-0504-5

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