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Pseudomonas donghuensis sp. nov., exhibiting high-yields of siderophore

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Abstract

A strain giving high-yields of siderophores, designated HYST, was isolated from the water of East Lake (also called Donghu Lake) of Wuhan in China. Strain HYST is Gram-stain negative, non-spore-forming and rod-shaped with polar flagella. Phylogenetic analysis based on 16S rRNA gene and the other three housekeeping genes (gyrB, rpoD and rpoB) indicated that strain HYST belongs to the genus Pseudomonas. Genomic DNA comparison experiments including DNA–DNA hybridization and whole-genome sequence similarities were performed between HYST and its phylogenetically most closely related type strains, all of the relatedness values are lower than the threshold to ascribe strain HYST to a known species. The major cellular fatty acids of strain HYST are C16:0, C17:0 cyclo, Summed feature 3 (C16:1 ω7c or/and C16:1 ω6c) and Summed feature 8 (C18:1 ω7c or C18:1 ω6c). Its predominant isoprenoid quinone was identified as Q-9, and the minor isoprenoid quinone was Q-8. Phylogenetic analysis together with genomic DNA comparison, phenotypic metabolic tests and chemotaxonomic analysis justified the proposal of strain HYST as a representative of a novel species, for which the name Pseudomonas donghuensis sp. nov. is proposed. The type strain is HYST ( = CCTCC AB 2012141T = NRRL B-59108T).

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Acknowledgments

This work was supported by the National Basic Research Program of China (973 Program, No. 2013CB933904), the National Natural Science Foundation of China (21272182), the National Infrastructure of Natural Resources for Science and Technology Program of China, the National Found for Fostering Talents of Basic Sciences (J1103513) and the research (Innovative) Fund of Laboratory Wuhan University. Thanks Xulu Chang for fatty acids HPLC identification, and thanks Wei Ni for offering API 20NE testing kits as gift. We are grateful to Kostas Konstantinidis for offering a script for ANI analysis between genomic sequences.

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  1. College of Life Sciences, China Center for Type Culture Collection (CCTCC), Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), State Key Laboratory of Virology, Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Wuhan University, Wuhan, 430072, People’s Republic of China

    Jingwei Gao, Guanfang Xie, Fang Peng & Zhixiong Xie

  2. Wuhan Huisheng Biotechnology Co., Ltd, Wuhan, 430042, People’s Republic of China

    Jingwei Gao

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  1. Jingwei Gao
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Correspondence to Zhixiong Xie.

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Jingwei Gao and Guanfang Xie have contributed equally.

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Gao, J., Xie, G., Peng, F. et al. Pseudomonas donghuensis sp. nov., exhibiting high-yields of siderophore. Antonie van Leeuwenhoek 107, 83–94 (2015). https://doi.org/10.1007/s10482-014-0306-1

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