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Screening of forest tree leaves from North Eastern Himalayan region as feed additives for modulating in vitro rumen fermentation and methanogenesis from total mixed ration

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Abstract

Sustainable feeding practices using agroforestry plants with high nutritional value can replace costlier feed ingredients, reduce food feed competition and lower greenhouse gas emissions in ruminants. Therefore, the present study was conducted to assess nutritional value and in vitro rumen fermentation parameters of various unconventional tree leaves from North Eastern Himalayan region to widen phytobiotic feed additive sources for mitigating ruminal methanogenesis. Leaves of Chillowance (Schima wallichii), Khasre (Ficus hirta), Tree bean (Parkia roxburghii), Parari (Schefflera wallichiana), Simontonia (Exbucklandia populnea), Nevaro (Ficus auriculata), Da qing shu (Ficus hookeri), Sohiong (Prunus nepalensis) and Rubber tree (Ficus elastica) were collected and individually incubated with cattle rumen liquor to determine their effect on in vitro methanogenesis, microbial biomass production and dry matter digestibility. The range of OM, CP, EE, T-CHO, NDF, ADF and cellulose content in the tree leaves were 83.6–97, 8.6–30.8, 1.9–4.8, 70.5–84.7, 43–61, 17–43 and 9–26.4% on DM basis, respectively. Following 24 h incubation, it was observed that Schefflera wallichiana had highest dry matter digestibility (68.2%) followed by Prunus nepalensis (63.5%), while methane production was found to be lowest in Ficus hookeri (18.3 mL/g of digestible dry matter/24 h). Based on the results of in vitro fermentation studies, three promising tree leaves, i.e. Prunus nepalensis, Ficus hookeri and Schefflera wallichiana, were further screened for their use as additives by replacing 25, 50 and 100 mg of control substrate (40% concentrate mixture and 60% paddy straw). Methane production was significantly reduced up to 20.8% with the replacement of control substrate by Ficus hookeri leaves at graded levels. Furthermore, an average reduction of 13.3% was observed in case of methane production when Ficus hookeri was added as an additive in comparison to Schefflera wallichiana and Prunus nepalensis leaves. Microbial biomass production and dry matter digestibility were enhanced up to 17.9 and 3.4%, whereas methane production was lowered by 15.3% with the addition of above tree leaves as additives irrespective of levels. Therefore, it can be implied that leaves of Schefflera wallichiana, Prunus nepalensis and Ficus hookeri have the potential to act as rumen modifiers for improving rumen fermentation and reducing enteric methanogenesis in ruminants.

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References

  • Agarwal N, Agarwal I, Kamra DN, Chaudhary LC (2000) Diurnal variation in the activities of hydrolytic enzymes in different fractions of rumen contents of Murrah buffaloes. J Appl Anim Res 18:73–80

    CAS  Google Scholar 

  • Agarwal N, Kamra DN, Chatterjee PN, Kumar R, Chaudhary LC (2008) In vitro methanogenesis, microbial profile and fermentation of green forages with buffalo rumen liquor as influenced by 2-bromoethanesulphonic acid. Asian Aust J Anim Sci 21(6):818–823

    CAS  Google Scholar 

  • Alberto MR, Manca de Nadra MC, Arena ME (2012) Influence of phenolic compounds on the growth and arginine deiminase system in a wine lactic acid bacterium. Braz J Microbiol 43(1):167–176

    CAS  PubMed  PubMed Central  Google Scholar 

  • AOAC (2005). Official methods of analysis. Association of Official Analytical Chemists, 18th edn, Maryland

  • Babiker EE, Juhaimi FAL, Ghafoor K, Abdoun KA (2018) Effect of drying methods on nutritional quality of young shoots and leaves of two Moringa species as non-conventional fodders. Agrofor Syst 92:717–729

    Google Scholar 

  • Bhatt RS, Sahoo A, Sarkar S, Saxena VK, Soni L, Sharma P, Gadekar YP (2021a) Dietary inclusion of nonconventional roughages for lowering enteric methane production and augmenting nutraceutical value of meat in cull sheep. Anim Feed Sci Technol 273:114832

    CAS  Google Scholar 

  • Bhatt RS, Sarkar S, Sahoo A, Sharma P, Soni L, Saxena VK, Soni A (2021b) Dietary inclusion of mature lemon grass and curry leaves affects nutrient utilization, methane reduction and meat quality in finisher lambs. Anim Feed Sci Technol 278:114979

    CAS  Google Scholar 

  • Bhatta R, Saravanan M, Baruah L, Prasad CS (2015) Effects of graded levels of tannin-containing tropical tree leaves on in vitro rumen fermentation, total protozoa and methane production. J Appl Microbiol 118:557–564

    CAS  PubMed  Google Scholar 

  • Bhatta R, Saravanan M, Baruah L, Malik P, Sampath K (2017) Nutrient composition, rate of fermentation and in vitro rumen methane output from tropical feedstuffs. J Agric Sci 155:171–183

    CAS  Google Scholar 

  • Blummel M, Makkar HPS, Becker K (1997) In vitro gas production: a technique revisited. J Anim Physiol Anim Nutr 77:24–34

    CAS  Google Scholar 

  • Buccioni A, Pauselli M, Viti C, Minieri S, Pallara G, Roscini V, Rapaccini S, Trabalza Marinucci M, Lupi P, Conte G, Mele M (2015) Milk fatty acid composition, rumen microbial population and animal performances in response to diets rich in linoleic acid supplemented with chestnut or quebracho tannins in dairy ewes. J Dairy Sci 98:1145–1156

    CAS  PubMed  Google Scholar 

  • Choudhary S, Santra A, Sarkar S, Das SK (2018) In vitro digestibility and fermentation kinetics of some north eastern Himalayan tree leaves using cattle rumen fluid as inoculum. Indian J Anim Sci 88:1085–1089

    CAS  Google Scholar 

  • Conway EJ (1962) Micro-diffusion analysis and volumetric error, 5th edn. Crossby Lockwood and Sons Ltd., London

    Google Scholar 

  • Datt C, Niranjan M, Chhabra A, Chatopadhyay K, Dhiman TR (2006) Forage yield, chemical composition and in vitro digestibility of different cultivars of maize (Zea mays L.). Ind J Dairy Sci 59:177–180

    CAS  Google Scholar 

  • Elaghandour MMY, Kholif AE, Salem AZM, Olafadehan OA, Kholif AM (2016) Sustainable anaerobic rumen methane and carbon dioxide productions from prickly pear cactus flour by organic acid salts addition. J Clean Prod 139:1362–1369

    Google Scholar 

  • Erwin ES, Marco GJ, Emery EM (1961) Volatile fatty acid analyses of blood and rumen fluid by gas chromatography. J Dairy Sci 44:1768–1771

    CAS  Google Scholar 

  • Flachowsky G, Von Soosten D, Meyer U (2017) Contributions to a sustainable production of food of animal origin, sustainable agriculture towards food security. Springer, Berlin, pp 197–227

    Google Scholar 

  • Frutos PG, Hervas RG, Giraldez FJ, Mantecon AR (2002) Condensed tannins content of several shrub species from a mountain area in northern Spain and its relationship to various indicators of nutritive value. Anim Feed Sci Technol 95:215–226

    CAS  Google Scholar 

  • Garcia-Gonzalez R, Lopez S, Fernandez M, Gonzale JS (2006) Effect of addition of some medicinal plants on methane production in a stimulating fermenter (RUSITEC). Int Congr Ser 1293:172–175

    CAS  Google Scholar 

  • Gupta R, Dutta TK, Kundu SS, Chatterjee A, Gautam M, Sarkar S (2016) Nutritional evaluation of tree leaves of Ayodhya hills of Purulia district, West Bengal. Indian J Animal Nutr 33:404–410

    CAS  Google Scholar 

  • Hariadi BT, Santoso B (2010) Evaluation of tropical plants containing tannin onin vitro methanogenesis and fermentation parameters using rumen fluid. J Sci Food Agric 90:456–461

    CAS  PubMed  Google Scholar 

  • Harson J, Rivera SF (2010) Collecting, processing and storage of plant materials for nutritional analysis. In: Vercoe PE, Makkar HPS, Schilink AC (eds) In vitro screening of plants resources for extra-nutritional attributes in Ruminants: nuclear and related methodologies. Springer, New York, pp 15–25

    Google Scholar 

  • Hundal JS, Wadhwa M, Bakshi MPS (2016) Methane mitigation potential of tannins and their impact on digestibility of nutrients in-vitro. Anim Nutr Feed Technol 16:505–513

    Google Scholar 

  • Jayanegara A, Wina E, Soliva CR, Marquardt S, Kreuzer M, Leiber F (2011) Dependence of forage quality and methanogenic potential of tropical plants on their phenolic fractions as determined by principal component analysis. Anim Feed Sci Technol 163:231–243

    CAS  Google Scholar 

  • Jayanegara A, Yogianto Y, Wina E, Sudarman A, Kondo M, Obitsu T, Kreuzer M (2020) Combination effects of plant extracts rich in tannins and saponins as feed additives for mitigating in vitro ruminal methane and ammonia formation. Animals 10:1531

    PubMed Central  Google Scholar 

  • Johnson KA, Johnson DE (1995) Methane emission from cattle. Anim Sci J 73:2483–2492

    CAS  Google Scholar 

  • Khazaal K, Dentino MT, Ribeiro JM, Orskov ER (1995) Prediction of apparent digestibility and voluntary intake of hays fed to sheep: comparison between using fibre components, in vitro digestibility or characteristics of gas production or nylon bag degradation. Anim Sci 61:527–538

    Google Scholar 

  • Krishnamoorthy U, Soller H, Steingass H, Menke KH (1995) Energy and protein evaluation of tropical feedstuffs for whole tract and ruminal digestion by chemical analyses and rumen inoculum studies in vitro. Anim Feed Sci Technol 52:177–188

    CAS  Google Scholar 

  • Makkar HPS (2003) Quantification of tannins in tree and shrub foliage. In: Makkar HPS (ed) A laboratory manual. Kulwere Academic Publisher/FAO/IAEA, Vienna, p 102

    Google Scholar 

  • Makkar HPS, Blümmel M, Becker K (1995) In vitro effects and interactions of tannins and saponins and fate of tannins in rumen. J Sci Food Agric 69:481–493

    CAS  Google Scholar 

  • Makkar HPS, Sen S, Blummel M, Becker K (1998) Effect of fractions containing saponins from Yucca schidigera Quillaja saponaria and Acacia auriculofprmis on rumen fermentation. J Agric Food Chem 46:4324–4328

    CAS  Google Scholar 

  • McAllister TA, Bae HD, Jones GA, Cheng KJ (1994) Microbial attachment and feed digestion in the rumen. J Anim Sci 72:3004–3018

    CAS  PubMed  Google Scholar 

  • McSweeny CS, Palmer B, McNeill DM, Krause DO (2001) Microbial interactions with tannins: nutritional consequences for ruminants. Anim Feed Sci Technol 91:83–93

    Google Scholar 

  • Menke KH, Steingass H (1988) Estimation of the energetic feed value obtained by chemical analysis and in vitro gas production using rumen fluid. Anim Res Dev 28:7–55

    Google Scholar 

  • National Research Council (1989) Nutrient requirements of dairy cattle sixth, rev. National academy press, Washington, DC

    Google Scholar 

  • Njidda AA, Nasiru A (2010) In vitro gas production and dry matter digestibility of tannin containing forages of semi-arid region of north-eastern Nigeria. Pak J Nutr 9:60–66

    CAS  Google Scholar 

  • Pal K, Patra AK, Sahoo A, Soren NM (2015a) Effects of nitrate and fumarate in tree leaves-based diets on nutrient utilization, rumen fermentation, microbial protein supply and blood profiles in sheep. Livest Sci 172:5–15

    Google Scholar 

  • Pal K, Patra AK, Sahoo A, Kumawat PK (2015b) Evaluation of several tropical tree leaves for methane production potential degradability and rumen fermentation in vitro. Livest Sci 180:98–105. https://doi.org/10.1016/j.livsci.2015.07.011

    Article  Google Scholar 

  • Pathak A, Narayan Dutta K, Pattanaik AK, Singh A, Narang A, Sharma KS (2015) Effect of condensed tannins supplementation from tanniferous tree leaves on methane production and efficiency of microbial biomass production in vitro. Anim Nutr Feed Technol 15:91–100

    Google Scholar 

  • Patra AK (2012) Enteric methane mitigation technologies for ruminant livestock: a synthesis of current research and future directions. Environ Monit Assess 184:1929–1952

    CAS  PubMed  Google Scholar 

  • Patra AK, Saxena J (2010) A new perspective on the use of plant secondary metabolites to inhibit methanogenesis in the rumen. Phytochemistry 71:1198–1222

    CAS  PubMed  Google Scholar 

  • Patra AK, Kamra DN, Agarwal N (2006) Effect of plant extracts on in vitro methanogenesis, enzyme activities and fermentation of feed in rumen liquor of buffalo. Anim Feed Sci Technol 128:276–291

    CAS  Google Scholar 

  • Sahoo A, Singh B, Sharma OP (2010) Evaluation of feeding value of Eupatorium adenophorum in combination with mulberry leaves. Livest Sci 136:175–183

    Google Scholar 

  • Salem AZM, Salem MZM, Gonzalez-Ronquillo M, Camacho LM, Cipriano M (2011) Major chemical constituents of Leucaena leucocephala and Salix babylonica leaf extracts. J Trop Agric 49:95–98

    CAS  Google Scholar 

  • Samanta AK, Das BK, Pawar S (2015) Nutritional evaluation of some promising top foliages of Aizawl district of Mizoram. Int J Res 2:496–501

    Google Scholar 

  • Santra A, Saikia A, Baruah KK (2012) Scope of rumen manipulation using medicinal plants to mitigate methane production. J Pharmacognosy 3:115–120

    Google Scholar 

  • Sarkar S, Mohini M, Nampoothiri VM, Mondal G, Pandita S (2016) Effect of tree leaves and malic acid supplementation to wheat straw based substrates on in vitro rumen fermentation parameters. Indian J Anim Nutr 33:421–426

    CAS  Google Scholar 

  • Sarkar S, Mohini M, Mondal G, Pandita S, Nampoothiri VM, Gautam M (2018) Effect of supplementing Aegle marmelos leaves on in vitro rumen fermentation and methanogenesis of diets varying in roughage to concentrate ratio. Indian J Anim Res 52:1180–1184

    Google Scholar 

  • Sarkar S, Mohini M, Sharma A, Tariq H, Pal RP (2021) Effect of supplementing Leucaena leucocephala leaves alone or in conjunction with malic acid on nutrient utilization, performance traits, and enteric methane emission in crossbred calves under tropical conditions. Trop Anim Health Prod 53:1–10

    Google Scholar 

  • Schofield P, Pitt RE, Pell AN (1994) Kinetics of fibre digestion from in vitro gas production. J Anim Sci 72:2980–2991

    CAS  PubMed  Google Scholar 

  • Shingfield KJ, Ahvenjarvi S, Toivonen V, Vanhatalo A, Huhtanen P, Griinari JM (2008) Effect of incremental levels of sunflower-seed oil in the diet on ruminal lipid metabolism in lactating cows. Brit J Nutr 99:971–983

    CAS  PubMed  Google Scholar 

  • Soltan YA, Morsy AS, Araujo RC, Elziat H, Sallam SMA Louvandini H, Abdalla AL (2011) Carvacrol and eugenol as modifiers of rumen microbial fermentation and methane production in vitro. In: Proceedings of 4th animal wealth research conference in the middle East and North Africa, pp 354–364

  • SPSS (2005). Statistical Package for Social Sciences, SPSS 14.0 for Windows. SPSS Inc, Chicago

  • Valenciaga D, Chongo B, Herrera RS, Torres V, Oramas A, Herrera M (2009) Effect of regrowth age on in vitro dry matter digestibility of pennisetum purpurecum cv. CUBACT-115. Cuba J Agric Sci 43:79–82

    Google Scholar 

  • VanKessel IAS, Russell JB (1996) The effect of pH on ruminal methanogenesis. FEMS Microbiol Ecol 20:205–210

    CAS  Google Scholar 

  • VanSoest PJ, Robertson JB, Lewis BA (1991) Methods for dietary fibre, neutral detergent fibre and non starch polysaccharides in relation to animal nutrition. J Dairy Sci 74:3583–3597

    CAS  Google Scholar 

  • Yusuf AO, Egbinola OO, Ekunseitan DA, Salem AZM (2020) Chemical characterization and in vitro methane production of selected agroforestry plants as dry season feeding of ruminants livestock. Agrofor Syst 94:1481–1489

    Google Scholar 

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Acknowledgements

The authors sincerely acknowledge the research facilities provided by the Director, ICAR-National Dairy Research Institute, Karnal and financial assistance from the Indian Council of Agricultural Research (ICAR), New Delhi, India.

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Authors and Affiliations

  1. ICAR-National Dairy Research Institute, Eastern Regional Station, Kalyani, West Bengal, India

    Shilpa Choudhary, Ashok Santra, Nirmala Muwel, Ajoy Mandal & Subrata Kumar Das

  2. ICAR-Central Sheep and Wool Research Institute, Avikanagar, Rajasthan, India

    Srobana Sarkar

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  1. Shilpa Choudhary
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  2. Ashok Santra
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  3. Nirmala Muwel
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  4. Srobana Sarkar
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Correspondence to Shilpa Choudhary.

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Choudhary, S., Santra, A., Muwel, N. et al. Screening of forest tree leaves from North Eastern Himalayan region as feed additives for modulating in vitro rumen fermentation and methanogenesis from total mixed ration. Agroforest Syst 96, 359–374 (2022). https://doi.org/10.1007/s10457-021-00724-5

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