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Electrospun PLGA Nanofiber Scaffolds Release Ibuprofen Faster and Degrade Slower After In Vivo Implantation

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Abstract

While delayed delivery of non-steroidal anti-inflammatory drugs (NSAIDs) has been associated with improved tendon healing, early delivery has been associated with impaired healing. Therefore, NSAID use is appropriate only if the dose, timing, and mode of delivery relieves pain but does not impede tissue repair. Because delivery parameters can be controlled using drug-eluting nanofibrous scaffolds, our objective was to develop a scaffold for local controlled release of ibuprofen (IBP), and characterize the release profile and degradation both in vitro and in vivo. We found that when incubated in vitro in saline, scaffolds containing IBP had a linear release profile. However, when implanted subcutaneously in vivo or when incubated in vitro in serum, scaffolds showed a rapid burst release. These data demonstrate that scaffold properties are dependent on the environment in which they are placed and the importance of using serum, rather than saline, for initial in vitro evaluation of biofactor release from biodegradable scaffolds.

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Acknowledgments

This work was supported by a Merit Grant from the Department of Veterans’ Affairs [O0979-R]; the National Institutes of Health [T32 AR007132, P30 AR050950]; and a National Science Foundation Graduate Research Fellowship.

Conflict of interest

The authors have no relevant disclosures related to this study.

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Authors and Affiliations

  1. Translational Musculoskeletal Research Center, Corporal Michael J. Crescenz Philadelphia VA Medical Center, 3900 University & Woodland Avenue, Philadelphia, PA, 19104, USA

    Corinne N. Riggin, Feini Qu, Dong Hwa Kim, Julianne Huegel, David R. Steinberg, Andrew F. Kuntz, Louis J. Soslowsky, Robert L. Mauck & Joseph Bernstein

  2. McKay Orthopaedic Research Lab, University of Pennsylvania, 424 Stemmler Hall, 36th Street & Hamilton Walk, Philadelphia, PA, 19104, USA

    Corinne N. Riggin, Feini Qu, Dong Hwa Kim, Julianne Huegel, David R. Steinberg, Andrew F. Kuntz, Louis J. Soslowsky, Robert L. Mauck & Joseph Bernstein

  3. Department of Bioengineering, University of Pennsylvania, Suite 240 Skirkanich Hall, 210 South 33rd Street, Philadelphia, PA, 19104, USA

    Corinne N. Riggin, Louis J. Soslowsky & Robert L. Mauck

  4. School of Veterinary Medicine, University of Pennsylvania, 3800 Spruce Street, Philadelphia, PA, 19104, USA

    Feini Qu

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  1. Corinne N. Riggin
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Correspondence to Joseph Bernstein.

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Associate Editor Kent Leach oversaw the review of this article.

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Riggin, C.N., Qu, F., Kim, D.H. et al. Electrospun PLGA Nanofiber Scaffolds Release Ibuprofen Faster and Degrade Slower After In Vivo Implantation. Ann Biomed Eng 45, 2348–2359 (2017). https://doi.org/10.1007/s10439-017-1876-7

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