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An In Vivo Rat Model of Artery Buckling for Studying Wall Remodeling

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Abstract

Theoretical modeling and in vitro experiments have demonstrated that arterial buckling is a possible mechanism for the development of artery tortuosity. However, there has been no report of whether artery buckling develops into tortuosity, partially due to the lack of in vivo models for long-term studies. The objective of this study was to establish an in vivo buckling model in rat carotid arteries for studying arterial wall remodeling after buckling. Rat left carotid arteries were transplanted to the right carotid arteries to generate buckling under in vivo pressure and were maintained for 1 week to examine wall remodeling and adaptation. Our results showed that a significant buckling was achieved in the carotid arterial grafts with altered wall stress. Cell proliferation and matrix metalloprotinease-2 (MMP-2) expression in the buckled arteries increased significantly compared with the controls. The tortuosity level of the grafts also slightly increased 1 week post-surgery, while there was no change in vessel dimensions, blood pressure, and blood flow velocity. The artery buckling model provides a useful tool for further study of the adaptation of arteries into tortuous shapes.

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Acknowledgments

This work was supported by internal funds from UTSA. It was also partially supported by CAREER award #0644646 from the National Science Foundation and grant R01HL095852 from the National Institute of Health. We thank Dr. Shu Q Liu of Northwestern University for his helpful suggestions. We also thank Dr. Yangming Xiao in our lab and staff in the Laboratory Animal Resources Center of UTSA, and Dr. Merry Lindsey’s lab for their kind help.

Conflict of interest

The authors have no conflict of interest.

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Authors and Affiliations

  1. Department of Mechanical Engineering, The University of Texas at San Antonio, San Antonio, TX, 78249, USA

    Jinzhou Zhang, Qin Liu & Hai-Chao Han

  2. Department of Cardiovascular Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an, China

    Jinzhou Zhang

Authors
  1. Jinzhou Zhang
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  2. Qin Liu
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  3. Hai-Chao Han
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Corresponding author

Correspondence to Hai-Chao Han.

Additional information

Associate Editor K. A. Athanasiou oversaw the review of this article.

Jinzhou Zhang and Qin Liu equally contributed to this work.

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Zhang, J., Liu, Q. & Han, HC. An In Vivo Rat Model of Artery Buckling for Studying Wall Remodeling. Ann Biomed Eng 42, 1658–1667 (2014). https://doi.org/10.1007/s10439-014-1017-5

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  • DOI: https://doi.org/10.1007/s10439-014-1017-5

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