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A microfluidic device for studying the production of reactive oxygen species and the migration in lung cancer cells under single or coexisting chemical/electrical stimulation

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Abstract

Reactive oxygen species (ROS) are known to play an important role in the development of cancer, and many exogenous sources are believed to be related to the formation of ROS. For example, electric field is one of such factors reported to stimulate the production of ROS. Moreover, electric field is also shown to induce cell migration, a phenomenon termed electrotaxis. In this paper, a microfluidic chip was developed for studying the production of ROS and the migration in lung cancer cells under single or coexisting chemical/electrical stimulation. This chip has two unique features: (1) Five relative concentrations of 0, 1/8, 1/2, 7/8, and 1 are achieved in the culture regions; (2) five different strengths of EFs are produced inside these culture areas. Lung cancer cells were seeded inside this biocompatible chip for investigating their response to different concentrations of H2O2, a chemical stimulus known to increase the production of ROS. Then, the effects of honokiol, a chemical stimulus, in combination with electric field, a physical stimulus, on lung cancer cells were examined. Finally, lung cancer cell migration was investigated under single or combined honokiol/electric field treatments. The current microfluidic chip provides an in vitro platform mimicking the physiological condition where cells are under circulating conditions and are subject to controllable chemical/physical stimuli.

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Acknowledgments

This work was financially supported by the Ministry of Science and Technology of Taiwan under Contract No. MOST 104-2311-B-002-026 (K. Y. Lo), No. MOST 104-2112-M-030-002 (Y. S. Sun), and the National Taiwan University Career Development Project (103R7888) (K. Y. Lo). The authors would like to thank Dr. Ji-Yen Cheng for help in fabricating microfluidic chips. The authors also thank the Center for Emerging Material and Advanced Devices, National Taiwan University, for the cell culture room facility.

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Authors and Affiliations

  1. Department of Agricultural Chemistry, National Taiwan University, Taipei, 10617, Taiwan, ROC

    Kai-Yin Lo & Shang-Ying Wu

  2. Department of Physics, Fu-Jen Catholic University, No. 510, Zhongzheng Rd., Xinzhuang Dist., New Taipei City, 24205, Taiwan, ROC

    Yung-Shin Sun

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  1. Kai-Yin Lo
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Correspondence to Yung-Shin Sun.

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Lo, KY., Wu, SY. & Sun, YS. A microfluidic device for studying the production of reactive oxygen species and the migration in lung cancer cells under single or coexisting chemical/electrical stimulation. Microfluid Nanofluid 20, 15 (2016). https://doi.org/10.1007/s10404-015-1683-0

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