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Combination of ultrasound and newly synthesized magnetic nanocapsules affects the temperature profile of CT26 tumors in BALB/c mice

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Abstract

Purpose

To investigate the effects of a combination of 3-MHz ultrasound waves with a new magnetic nanocapsule containing 5-fluorouracil (5-Fu) on the temperature profile of a mouse colon tumor (CT26) in BALB/c mice.

Methods

Firstly, 5-Fu-loaded magnetic nanocapsules were synthesized using a multiple emulsion solvent evaporation procedure. Magnetic resonance imaging (MRI) was performed to evaluate the efficiency of nanocapsule localization in the tumor during magnetic drug targeting (MDT). Tumors were separately exposed to 3-MHz ultrasound waves at the intensities of 0.1, 0.3, 0.5, and 1 W/cm2 for 10 min in the absence and presence of nanocapsules. The temperature of the tumor was recorded at 1-min intervals.

Results

The effective diameter of the nanocapsules was approximately 70 nm, and it was demonstrated that magnetic nanoparticles were well dispersed inside the nanocapsules. MRI confirmed that the magnetic nanocapsules were successfully targeted to the tumor after accomplishing MDT. Temperature change due to sonication of the tumor was strongly intensity dependent. Moreover, temperature–time curves revealed that the magnetic nanocapsules significantly affected the temperature rise profile of a sonicated tumor.

Conclusion

Data presented in this study would be helpful to develop an ultrasound-mediated MDT procedure so that temperature changes of the tumor and its surrounding normal tissues may be controllable.

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Acknowledgments

This work was supported by a Grant (No. 15026) from the School of Medicine at Iran University of Medical Sciences (IUMS). We should all be quite thankful for those reviewers who helped us to correct the errors in the initial version of our manuscript.

Conflict of interest

Ali Shakeri-Zadeh, Samide Khoei, Sepideh Khoee, Alimohammad Sharifi, and Mohammad Bagher Shiran declare that they have no conflict of interest.

Ethical standard

All institutional and national guidelines for the care and use of laboratory animals were followed.

Author information

Authors and Affiliations

  1. Medical Physics Department, School of Medicine, Iran University of Medical Sciences (IUMS), PO Box 14155-5983, Tehran, Iran

    Ali Shakeri-Zadeh, Samideh Khoei & Mohammad-Bagher Shiran

  2. Razi Drug Research Centre, Iran University of Medical Sciences, Tehran, Iran

    Samideh Khoei

  3. Polymer Chemistry Department, School of Sciences, University of Tehran, Tehran, Iran

    Sepideh Khoee & Ali Mohammad Sharifi

  4. Pharmacology Department, School of Medicine, Iran University of Medical Sciences, Tehran, Iran

    Ali Mohammad Sharifi

Authors
  1. Ali Shakeri-Zadeh
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  2. Samideh Khoei
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  3. Sepideh Khoee
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  4. Ali Mohammad Sharifi
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  5. Mohammad-Bagher Shiran
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Corresponding authors

Correspondence to Ali Shakeri-Zadeh or Mohammad-Bagher Shiran.

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Shakeri-Zadeh, A., Khoei, S., Khoee, S. et al. Combination of ultrasound and newly synthesized magnetic nanocapsules affects the temperature profile of CT26 tumors in BALB/c mice. J Med Ultrasonics 42, 9–16 (2015). https://doi.org/10.1007/s10396-014-0558-4

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  • DOI: https://doi.org/10.1007/s10396-014-0558-4

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