Skip to main content

Advertisement

SpringerLink
Log in

A reliable animal model to simulate benign esophageal strictures in children

  • Original Article
  • Published:
Esophagus Aims and scope Submit manuscript

Abstract

Background

Benign esophageal strictures are common among children, especially children under 5 years of age. There is no standard treatment for refractory benign esophageal stricture, and it is quite challenging to care for children. The aim of this research is to establish an animal model of benign esophageal stricture with a custom balloon catheter, offering a cornerstone for exploring effective treatment methods.

Methods

12 rabbits were divided into the injury group and the control group. The sodium hydroxide or normal saline was given to the injury and control groups using a 12 F single catheter with two separated balloons under fluoroscopy monitoring, respectively, keeping for 40 s before the liquid was eliminated. After the operation, follow-up examinations evaluated the rabbits’ daily diet consumption, body weight, and behavior changes. All rabbits were killed on the 28th day, and the morphological and histological features were recorded.

Results

Radiological and histological examinations revealed that esophageal stricture had been induced successfully in the injury group. The esophageal diameter of the injury group decreased more than 50 %, and the damage score of it was higher than the control’s. Moreover, the ratio between the residual lumen area and total esophageal cross-sectional area was lower in the injury group.

Conclusions

Benign esophageal stricture can be established successfully with our custom balloon catheter. This technique simplifies the procedure and makes the degree and area of stenosis easy to control.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. Best C, Sudel B, Foker JE, et al. Esophageal stenting in children: indications, application, effectiveness, and complications. Gastrointest Endosc. 2009;70:1248–53.

    Article  PubMed  Google Scholar 

  2. Zhang C, Zhou X, Yu L, et al. Endoscopic therapy in the treatment of caustic esophageal stricture: a retrospective case series study. Dig Endosc. 2013;25:490–5.

    Article  PubMed  Google Scholar 

  3. Christesen HB. Epidemiology and prevention of caustic ingestion in children. Acta Paediatr. 1994;83:212–5.

    Article  CAS  PubMed  Google Scholar 

  4. Uygun I, Arslan MS, Aydogdu B, et al. Fluoroscopic balloon dilatation for caustic esophageal stricture in children: an 8-year experience. J Pediatr Surg. 2013;48:2230–4.

    Article  PubMed  Google Scholar 

  5. Ruthmann O, Richter S, Fischer A, et al. Biliary stenting of an iatrogenic esophageal perforation following corrosive esophagitis in a 5-year-old child. Endoscopy. 2009;41(Suppl 2):E325–6.

    Article  PubMed  Google Scholar 

  6. Zheng J, Chang Z, Liu Z, et al. Retrievable Z-stents for the treatment of refractory corrosive esophageal strictures in children. Eur J Pediatr Surg. 2015;25:160–4.

    Article  PubMed  Google Scholar 

  7. Saito Y, Tanaka T, Andoh A, et al. Usefulness of biodegradable stents constructed of poly-l-lactic acid monofilaments in patients with benign esophageal stenosis. World J Gastroenterol. 2007;13:3977–80.

    Article  PubMed  PubMed Central  Google Scholar 

  8. Okata Y, Hisamatsu C, Hasegawa T, et al. Development of a model of benign esophageal stricture in rats: the optimal concentration of sodium hydroxide for stricture formation. Pediatr Surg Int. 2011;27:73–80.

    Article  PubMed  Google Scholar 

  9. Bustamante TF, Lourencao PL, Higa KL, et al. The use of mitomycin C in caustic esophagitis in rats. Acta Cir Bras. 2013;28:136–41.

    Article  PubMed  Google Scholar 

  10. Nonaka K, Miyazawa M, Ban S, et al. Different healing process of esophageal large mucosal defects by endoscopic mucosal dissection between with and without steroid injection in an animal model. BMC Gastroenterol. 2013;13:72.

    Article  PubMed  PubMed Central  Google Scholar 

  11. Ocakci A, Coskun O, Tumkaya L, et al. Beneficial effects of Ebselen on corrosive esophageal burns of rats. Int J Pediatr Otorhinolaryngol. 2006;70:45–52.

    Article  PubMed  Google Scholar 

  12. Kaygusuz I, Celik O, Ozkaya OO, et al. Effects of interferon-alpha-2b and octreotide on healing of esophageal corrosive burns. Laryngoscope. 2001;111:1999–2004.

    Article  CAS  PubMed  Google Scholar 

  13. Bautista A, Tojo R, Varela R, et al. Effects of prednisolone and dexamethasone on alkali burns of the esophagus in rabbit. J Pediatr Gastroenterol Nutr. 1996;22:275–83.

    Article  CAS  PubMed  Google Scholar 

  14. Hwang JC, Jin B, Kim JH, et al. Esophageal stricture induced by an ultraslim upper endoscope in a novel rabbit model of corrosive injury. Scand J Gastroenterol. 2014;49:30–4.

    Article  PubMed  Google Scholar 

  15. Davis WM, Madden JW, Peacock EE Jr. A new approach to the control of esophageal stenosis. Ann Surg. 1972;176:469–76.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  16. Numanoglu KV, Tatli D, Bektas S, et al. Efficacy of keratinocyte growth factor (palifermin) for the treatment of caustic esophageal burns. Exp Ther Med. 2014;8:1087–91.

    PubMed  PubMed Central  Google Scholar 

  17. Kapisiz A, Karabulut R, Sonmez K, et al. Effect of stent placement, balloon or cutting balloon dilatation on stricture formation after caustic esophageal burn in rats. Eur J Pediatr Surg. 2011;21:258–62.

    Article  CAS  PubMed  Google Scholar 

  18. Guven A, Demirbag S, Uysal B, et al. Effect of 3-amino benzamide, a poly (adenosine diphosphate-ribose) polymerase inhibitor, in experimental caustic esophageal burn. J Pediatr Surg. 2008;43:1474–9.

    Article  PubMed  Google Scholar 

  19. Yukselen V, Vardar E, Yukselen O, et al. Colchicine in experimental alkaline burns of the rat esophagus: an old drug, a new indication? Pediatr Surg Int. 2006;22:363–8.

    Article  PubMed  Google Scholar 

  20. Senturk E, Sen S, Pabuccu E, et al. New experimental corrosive esophagitis model in rats. Pediatr Surg Int. 2010;26:257–61.

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

This work was supported by grant from the production-study-research-medical cooperation projects, Science and Technology Commission of Shanghai Municipality (No. 13DZ1942705) and Natural Science Fund of China (No. 81070134 and 30772349).

Author information

Authors and Affiliations

  1. Department of Pediatric Heart Center, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, 1665 Kongjiang Avenue, Shanghai, 200092, China

    Yang Liu, Hui Dong Liu, Li Juan Xu, Sun Chen & Kun Sun

Authors
  1. Yang Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hui Dong Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Li Juan Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Sun Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Kun Sun
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Kun Sun.

Ethics declarations

Ethical statement

All institutional and national guidelines for the care and use of laboratory animals were followed. The complete experiment was only undertaken after project approval from the Ethics Committee of the Xinhua Hospital affiliated to Shanghai Jiaotong University School of Medicine in China. All institutional and national guidelines for the care and use of laboratory animals were followed.

Conflict of interest

All the authors listed have declared that they have no conflict of interest.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Liu, Y., Liu, H.D., Xu, L.J. et al. A reliable animal model to simulate benign esophageal strictures in children. Esophagus 13, 156–162 (2016). https://doi.org/10.1007/s10388-015-0508-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10388-015-0508-2

Keywords

Search

Navigation