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Aqueous humour levels of ghrelin in exfoliation syndrome and exfoliation glaucoma patients

  • Clinical Investigation
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Abstract

Purpose

To investigate levels of ghrelin in the aqueous humour (AqH) of patients with exfoliation syndrome and exfoliation glaucoma and compare them to levels of ghrelin in control subjects.

Methods

This cross-sectional study involved 15 patients with exfoliation syndrome, 8 with exfoliation glaucoma and 12 control subjects for whom cataract surgery was indicated. The AqH was aspirated from the anterior chamber with a 27-G needle under sterile conditions prior to tissue manipulation. Ghrelin levels were quantified using radioimmunassay kits.

Results

Levels of ghrelin in the AqH were 187.87 ± 80.1 pg/mL in the eyes exhibiting exfoliation syndrome, 98.53 ± 50.9 pg/mL in the eyes exhibiting exfoliation glaucoma and 111.40 ± 77.5 pg/mL in the controls. Ghrelin level of patients with exfoliation syndrome were significantly higher than those of patients with exfoliation glaucoma and the controls (P < 0.05). Ghrelin levels of patients with exfoliation glaucoma were lower than those of the controls but were not reach statistically significant (P > 0.05). Age, gender and IOP did not have a significant effect on ghrelin levels in patients with exfoliation syndrome and exfoliation glaucoma.

Conclusion

This study is the first to report elevated levels of ghrelin in the AqH in eyes exhibiting exfoliation syndrome. Findings suggest ghrelin might play role in the etiopathogenesis of exfoliation syndrome to exfoliation glaucoma.

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References

  1. Ritch R, Schlötzer-Schrehardt U. Exfoliation syndrome. Surv Ophthalmol. 2001;45:265–315.

    Article  CAS  PubMed  Google Scholar 

  2. Naumann GO, Schlötzer-Schrehardt U, Küchle M. Pseudoexfoliation syndrome for the comprehensive ophthalmologist. Intraocular and systemic manifestations. Ophthalmology. 1998;105:951–68.

    Article  CAS  PubMed  Google Scholar 

  3. Koliakos GG, Konstas AG, Triantos A, Ritch R. Increased growth factor activity in the aqueous humour of patients with exfoliation syndrome. Graefes Arch Clin Exp Ophthalmol. 2000;238:491–5.

    Article  CAS  PubMed  Google Scholar 

  4. Doğu B, Yüksel N, Cekmen MB, Cağlar Y. Aqueous humor and serum erythropoietin levels in patients with pseudoexfoliation syndrome and pseudoexfoliative glaucoma. Int Ophthalmol. 2010;30:669–74.

    Article  PubMed  Google Scholar 

  5. Browne JG, Ho SL, Kane R, Oliver N, Clark AF, O’Brien CJ, et al. Connective tissue growth factor is increased in pseudoexfoliation glaucoma. Invest Ophthalmol Vis Sci. 2011;52:3660–6.

    Article  CAS  PubMed  Google Scholar 

  6. Tosun M, Erdurmus M, Bugdayci G, Celebi S, Alcelik A. Aqueous humour and serum concentration of asymmetric dimethyl arginine in pseudoexfoliation syndrome. Br J Ophthalmol. 2012;96:1137–40.

    Article  PubMed  Google Scholar 

  7. Gottanka J, Flugel-Koch C, Martus P, Johnson DH, Lu¨tjen-Drecoll E. Correlation of pseudoexfoliative material and optic nerve damage in pseudoexfoliation syndrome. Invest Ophthalmol Vis Sci. 1997;38:2435–46.

    CAS  PubMed  Google Scholar 

  8. Henry JC, Krupin T, Schmitt M, Lauffer J, Miller E, Ewing MQ, et al. Long-term follow-up of pseudoexfoliation and the development of elevated intraocular pressure. Ophthalmology. 1987;94:545–52.

    Article  CAS  PubMed  Google Scholar 

  9. Conway RM, Schlotzer-Schrehardt U, Kuchle M, Naumann GOH. Pseudoexfoliation syndrome: pathological manifestations of relevance to intraocular surgery. Clin Exp Ophthalmol. 2004;32:199–210.

    Article  Google Scholar 

  10. Kojima M, Hosoda H, Date Y, Nakazato M, Matsuo H, Kangawa K. Ghrelinis a growth-hormone-releasing acylated peptide from stomach. Nature. 1999;402:656–60.

    Article  CAS  PubMed  Google Scholar 

  11. Bednarek MA, Feighner SD, Pong SS, McKee KK, Hreniuk DL, Silva MV, et al. Structure-function studies on the new growth hormone-releasing peptide, ghrelin: minimal sequence of ghrelin necessary for activation of growth hormone secretagogue receptor 1a. J Med Chem. 2000;43:4370–6.

    Article  CAS  PubMed  Google Scholar 

  12. Azevedo-Pinto S, Pereira-Silva P, Rocha-Sousa A. Ghrelin in ocular pathophysiology: from the anterior to the posterior segment. Peptides. 2013;28:12–9.

    Article  Google Scholar 

  13. Rocha-Sousa A, Saraiva J, Henriques-Coelho T, Falcao-Reis F, Correia-Pinto J, Leite-Moreira AF. Ghrelin as a novel locally produced relaxing peptide of the iris sphincter and dilator muscles. Exp Eye Res. 2006;83:1179–87.

    Article  CAS  PubMed  Google Scholar 

  14. Rocha-Sousa A, Alves-Faria P, Falcao-Pires I, Falcao-Reis F, Leite-Moreira AF. Analyses of aqueous humour ghrelin levels of eyes with and without glaucoma. Br J Ophthalmol. 2009;93:131–2.

    Article  CAS  PubMed  Google Scholar 

  15. Katsanos A, Dastiridou A, Georgoulias P, Cholevas P, Kotoula M, Tsironi EE. Plasma and aqueous humour levels of ghrelin in open-angle glaucoma patients. Clin Exp Ophthalmol. 2011;39:324–9.

    Article  Google Scholar 

  16. Küchle M, Ho TS, Nguyen NX, Hannappel E, Naumann GOH. Protein quantification and electrophoresis in aqueous humor of pseudoexfoliation eyes. Invest Ophthalmol Vis Sci. 1994;35:748–52.

    PubMed  Google Scholar 

  17. Tripathi RC, Borisuth NSC, Li J, Tripathi AJ, Tripathi BJ. Growth factors in the aqueous humour and their clinical significance. J Glaucoma. 1994;3:248–58.

    Article  CAS  PubMed  Google Scholar 

  18. Ho SL, Dogar GF, Wang J, Crean J, Wu QD, Oliver N, et al. Elevated aqueous humour tissue inhibitor of matrix metalloproteinase-1 and connective tissue growth factor in pseudoexfoliation syndrome. Br J Ophthalmol. 2005;89:169–73.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  19. Shinde UA, Desai KM, Yu C, Gopalakrishnan V. Nitric oxide synthase inhibition exaggerates the hypotensive response to ghrelin: role of calcium-activated potassium channels. J Hypertens. 2005;23:779–84.

    Article  CAS  PubMed  Google Scholar 

  20. Rocha-Sousa A, Pereira-Silva P, Azevedo-Pinto S, Rodrigues-Araujo J,Tavares-Silva M, Pinho S. Ghrelin’s expression in the eye and its implicationin the reduction of intraocular pressure. Acta Ophthalmol. 2012;90.

  21. Rocha-Sousa A, Tavares-Silva M, Pereira-Silva P. Ghrelin is expressed in the ciliary body and presents a significant and sustained hypotensive effect in acute glaucoma models. Peptides. 2013;47:12–9.

    Article  PubMed  Google Scholar 

  22. Mikropoulos DG, Mallini P, Michopoulou A, Gianopoulos T, Arranz-Marquez E, Koliakos GG, et al. Asymmetric dimethyloarginin (ADMA) concentration in the aqueous humor of patients with exfoliation syndrome or exfoliative glaucoma. Curr Eye Res. 2013;38:266–70.

    Article  CAS  PubMed  Google Scholar 

  23. Banks WA. The blood–brain barrier: connecting the gut and the brain. Regul Pept. 2008;149:11–4.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  24. Date Y, Murakami N, Toshinai K, Matsukura S, Niijima A, Matsuo H, Kangawa K, et al. The role of the gastric afferent vagal nerve in ghrelin-induced feeding and growth hormone secretion in rats. Gastroenterology. 2002;123:1120–8.

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This study was supported by Turkish Ophthalmology Society.

Conflicts of interest

A. Vural Özeç, None; A. Dursun, None; M. İ. Toker, None; Y. Demirci, None; A. Topalkara, None; H. Erdoğan, None; M. K. Arıcı, None; T. Ersalcan, None.

Author information

Authors and Affiliations

  1. Department of Ophthalmology, Cumhuriyet University School of Medicine, Sivas, 58140, Turkey

    Ayşe Vural Özeç, Ayhan Dursun, Mustafa İlker Toker, Yüksel Demirci, Aysen Topalkara, Haydar Erdoğan & Mustafa Kemal Arıcı

  2. Department of Nuclear Medicine, Cumhuriyet University School of Medicine, Sivas, Turkey

    Taner Ersalcan

Authors
  1. Ayşe Vural Özeç
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  2. Ayhan Dursun
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  3. Mustafa İlker Toker
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  4. Yüksel Demirci
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  5. Aysen Topalkara
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  6. Haydar Erdoğan
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  7. Mustafa Kemal Arıcı
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  8. Taner Ersalcan
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Correspondence to Ayşe Vural Özeç.

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Özeç, A.V., Dursun, A., Toker, M.İ. et al. Aqueous humour levels of ghrelin in exfoliation syndrome and exfoliation glaucoma patients. Jpn J Ophthalmol 58, 348–352 (2014). https://doi.org/10.1007/s10384-014-0322-8

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  • DOI: https://doi.org/10.1007/s10384-014-0322-8

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