Abstract
Nitrogen (N) and phosphorus (P) fertilization are effective methods for increasing forest productivity and have been widely used in the management of forest plantations. However, how changes in the N:P ratio affect the sequestration of carbon in forests is unclear. We measured the net primary production (NPP) based on plot inventory, soil respiration (RS), autotrophic respiration (RA), and heterotrophic respiration (RH) using static chamber with GC method after trenching to compare the effects of low N fertilization (50 kg N ha−1 year−1), high N fertilization (100 kg N ha−1 year−1), P fertilization (50 kg P ha−1 year−1), low N plus P fertilization, high N plus P fertilization, and an unfertilized control on net ecosystem productivity (NEP). The NEP was significantly higher with 39–60% more under N and P fertilization than that of control. However, the mechanisms contributing to the difference varied among different treatments. Specifically, the RH was unchanged with P fertilization but significantly lower with N fertilization, while NPP was significantly higher with P or N fertilization. The NEP was higher with N plus P fertilization than with either N or P fertilization, although the interactive effect was not significant. Our findings suggested that P fertilization may be an effective method of improving timber yield and that adding both N and P fertilizers at a higher ratio may favor ecosystem carbon sequestration in P-limited stands.
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References
Albaugh TJ, Rubilar RA, Fox TR, Lee Allen H, Urrego JB, Zapata M, Stape JL (2015) Response of Eucalyptus grandis in Colombia to mid-rotation fertilization is dependent on site and rate but not frequency of application. For Ecol Manag 350:30–39. https://doi.org/10.1016/j.foreco.2015.04.030
Alvarez-Clare S, Mack MC, Brooks M (2013) A direct test of nitrogen and phosphorus limitation to net primary productivity in a lowland tropical wet forest. Ecology 94:1540–1551. https://doi.org/10.1890/12-2128.1
Bai R, Wang JT, Deng Y, He JZ, Feng K, Zhang LM (2017) Microbial community and functional structure significantly varied among distinct types of paddy soils but responded differently along gradients of soil depth layers. Front Microbiol 8:945. https://doi.org/10.3389/fmicb.2017.00945
Bi J, Blanco JA, Seely B, Kimmins JP, Ding Y, Welham C (2007) Yield decline in Chinese-fir plantations: a simulation investigation with implications for model complexity Canadian. J For Res 37:1615–1630. https://doi.org/10.1139/x07-018
Bowden RD, Davidson E, Savage K, Arabia C, Steudler P (2004) Chronic nitrogen additions reduce total soil respiration and microbial respiration in temperate forest soils at the Harvard Forest. For Ecol Manag 196:43–56. https://doi.org/10.1016/j.foreco.2004.03.011
Burton AJ, Pregitzer KS, Crawford JN, Zogg GP, Zak DR (2004) Simulated chronic NO3 −deposition reduces soil respiration in northern hardwood forests. Glob Change Biol 10:1080–1091. https://doi.org/10.1111/j.1365-2486.2004.00737.x
Chen FS, Niklas KJ, Liu Y, Fang XM, Wan SZ, Wang H (2015) Nitrogen and phosphorus additions alter nutrient dynamics but not resorption efficiencies of Chinese fir leaves and twigs differing in age. Tree Physiol 35:1106–1117. https://doi.org/10.1093/treephys/tpv076
Cleveland CC, Townsend AR (2006) Nutrient additions to a tropical rain forest drive substantial soil carbon dioxide losses to the atmosphere. Proc Natl Acad Sci USA 103:10316–10321. https://doi.org/10.1073/pnas.0600989103
Cleveland CC, Townsend AR, Schmidt SK (2002) Phosphorus limitation of microbial processes in moist tropical forests: evidence from short-term laboratory incubations and field studies. Ecosystems 5:0680–0691. https://doi.org/10.1007/s10021-002-0202-9
Cleveland CC, Townsend AR, Taylor P, Alvarez-Clare S, Bustamante MM, Chuyong G, Dobrowski SZ, Grierson P, Harms KE, Houlton BZ, Marklein A, Parton W, Porder S, Reed SC, Sierra CA, Silver WL, Tanner EV, Wieder WR (2011) Relationships among net primary productivity, nutrients and climate in tropical rain forest: a pan-tropical analysis. Ecol Lett 14:939–947. https://doi.org/10.1111/j.1461-0248.2011.01658.x
Corbeels M, McMurtrie RE, Pepper DA, Mendham DS, Grove TS, O’Connell AM (2005) Long-term changes in productivity of eucalypt plantations under different harvest residue and nitrogen management practices: a modelling analysis. For Ecol Manag 217:1–18. https://doi.org/10.1016/j.foreco.2005.05.057
De Vries W, Reinds GJ, Vel E (2003) Intensive monitoring of forest ecosystems in Europe. For Ecol Manag 174:97–115
Du Z, Wang W, Zeng W, Zeng H (2014) Nitrogen deposition enhances carbon sequestration by plantations in northern China. PLoS ONE 9:e87975
Elser JJ, Bracken ME, Cleland EE, Gruner DS, Harpole WS, Hillebrand H, Ngai JT, Seabloom EW, Shurin JB, Smith JE (2007) Global analysis of nitrogen and phosphorus limitation of primary producers in freshwater, marine and terrestrial ecosystems. Ecol Lett 10:1135–1142. https://doi.org/10.1111/j.1461-0248.2007.01113.x
Esberg C, du Toit B, Olsson R, Ilstedt U, Giesler R (2009) Microbial responses to P addition in six South African forest soils. Plant Soil 329:209–225. https://doi.org/10.1007/s11104-009-0146-3
Euskirchen ES, McGuire AD, Kicklighter DW, Zhuang Q, Clein JS, Dargaville RJ, Dye DG, Kimball JS, McDonald KC, Melillo JM, Romanovsky VE, Smith NV (2006) Importance of recent shifts in soil thermal dynamics on growing season length, productivity, and carbon sequestration in terrestrial high-latitude ecosystems. Glob Change Biol 12:731–750. https://doi.org/10.1111/j.1365-2486.2006.01113.x
Fan H, Wu J, Liu W, Yuan Y, Huang R, Liao Y, Li Y (2014) Nitrogen deposition promotes ecosystem carbon accumulation by reducing soil carbon emission in a subtropical forest. Plant Soil 379:61–371. https://doi.org/10.1007/s11104-014-2076-y
Fisher RF, Binkley D (2000) Ecology and management of forest soils. Wiley, New York
Gower ST, Krankina O, Olson RJ, Apps M, Linder S, Wang C (2001) Net primary production and carbon allocation patterns of boreal forest ecosystems. Ecol Appl 11:1395–1411. https://doi.org/10.1890/1051-0761(2001)011%5b1395:nppaca%5d2.0.co;2
Högberg P (2012) What is the quantitative relation between nitrogen deposition and forest carbon sequestration? Glob Change Biol 18:1–2. https://doi.org/10.1111/j.1365-2486.2011.02553.x
Högberg P, Fan H, Quist M, Binkley DAN, Tamm CO (2006) Tree growth and soil acidification in response to 30 years of experimental nitrogen loading on boreal forest. Glob Change Biol 12:489–499. https://doi.org/10.1111/j.1365-2486.2006.01102.x
Ilstedt U, Singh S (2005) Nitrogen and phosphorus limitations of microbial respiration in a tropical phosphorus-fixing acrisol (ultisol) compared with organic compost. Soil Biol Biochem 37:1407–1410. https://doi.org/10.1016/j.soilbio.2005.01.002
Janssens IA, Dieleman W, Luyssaert S, Subke JA, Reichstein M, Ceulemans R, Ciais P, Dolman AJ, Grace J, Matteucci G, Papale D, Piao SL, Schulze ED, Tang J, Law BE (2010) Reduction of forest soil respiration in response to nitrogen deposition. Nat Geosci 3:315–322. https://doi.org/10.1038/ngeo844
Kuzyakov Y (2006) Sources of CO2 efflux from soil and review of partitioning methods. Soil Biol Biochem 38:425–448. https://doi.org/10.1016/j.soilbio.2005.08.020
Laclau JP, Ranger J, Deleporte P, Nouvellon Y, Saint-André L, Marlet S, Bouillet JP (2005) Nutrient cycling in a clonal stand of Eucalyptus and an adjacent savanna ecosystem in Congo. For Ecol Manag 210:375–391. https://doi.org/10.1016/j.foreco.2005.02.028
LeBauer DS, Treseder KK (2008) Nitrogen limitation of net primary productivity in terrestrial ecosystems is globally distributed. Ecology 89:371–379
Li Y, Zhang JG, Duan AG, Xiang CW (2010) Selection of biomass estimation models for Chinese fir plantation. J Appl Ecol (Abstract in English) 21(12):3036–3046
Liu L, Zhang T, Gilliam FS, Gundersen P, Zhang W, Chen H, Mo JM (2013) Interactive effects of nitrogen and phosphorus on soil microbial communities in a tropical forest. PLoS ONE 8:e61188. https://doi.org/10.1371/journal.pone.0061188
Lovett GM, Arthur MA, Weathers KC, Fitzhugh RD, Templer PH (2013) Nitrogen addition increases carbon storage in soils, but not in trees, in an Eastern US deciduous forest. Ecosystems 16:980–1001. https://doi.org/10.1007/s10021-013-9662-3
Ma X, Heal KV, Liu A, Jarvis PG (2007) Nutrient cycling and distribution in different-aged plantations of Chinese fir in southern China. For Ecol Manag 243:61–74. https://doi.org/10.1016/j.foreco.2007.02.018
Maaroufi NI, Nordin A, Hasselquist NJ, Bach LH, Palmqvist K, Gundale MJ (2015) Anthropogenic nitrogen deposition enhances carbon sequestration in boreal soils. Glob Change Biol 21:3169–3180. https://doi.org/10.1111/gcb.12904
Magnani F, Mencuccini M, Borghetti M, Berbigier P, Berninger F, Delzon S, Grelle A, Hari P, Jarvis PG, Kolari P, Kowalski AS, Lankreijer H, Law BE, Lindroth A, Loustau D, Manca G, Moncrieff JB, Rayment M, Tedeschi V, Valentini R, Grace J (2007) The human footprint in the carbon cycle of temperate and boreal forests. Nature 447:848–850. https://doi.org/10.1038/nature05847
Marklein AR, Houlton BZ (2012) Nitrogen inputs accelerate phosphorus cycling rates across a wide variety of terrestrial ecosystems. New Phytol 193:696–704. https://doi.org/10.1111/j.1469-8137.2011.03967.x
Mo J, Zhang W, Zhu W, Gundersen P, Fang Y, Li D, Wang H (2007) Nitrogen addition reduces soil respiration in a mature tropical forest in southern China. Glob Change Biol 14:403–412. https://doi.org/10.1111/j.1365-2486.2007.01503.x
Nilsson LO, Wallander H (2003) Production of external mycelium by ectomycorrhizal fungi in a norway spruce forest was reduced in response to nitrogen fertilization. New Phytol 158:409–416. https://doi.org/10.1046/j.1469-8137.2003.00728.x
Olsson P, Linder S, Giesler R, Högberg P (2005) Fertilization of boreal forest reduces both autotrophic and heterotrophic soil respiration. Glob Change Biol 11:1745–1753. https://doi.org/10.1111/j.1365-2486.2005.001033.x
Peñuelas J, Sardans J, Rivas-ubach A, Janssens IA (2012) The human-induced imbalance between C, N and P in Earth’s life system. Glob Change Biol 18:3–6. https://doi.org/10.1111/j.1365-2486.2011.02568.x
Perring MP, Hedin LO, Levin SA, McGroddy M, de Mazancourt C (2008) Increased plant growth from nitrogen addition should conserve phosphorus in terrestrial ecosystems. Proc Natl Acad Sci USA 105:1971–1976. https://doi.org/10.1073/pnas.0711618105
Phillips RP, Fahey TJ (2007) Fertilization effects on fineroot biomass, rhizosphere microbes and respiratory fluxes in hardwood forest soils. New Phytol 176:655–664. https://doi.org/10.1111/j.1469-8137.2007.02204.x
Reed SC, Cleveland CC, Townsend AR (2007) Controls over leaf litter and soil nitrogen fixation in two lowland tropical rain forests. Biotropica 39:585–592. https://doi.org/10.1111/j.1744-7429.2007.00310.x
Reich PB, Oleksyn J, Wright IJ (2009) Leaf phosphorus influences the photosynthesis–nitrogen relation: a cross-biome analysis of 314 species. Oecologia 160:207–212. https://doi.org/10.1007/s00442-009-1291-3
Rubilar RA, Lee Allen H, Fox TR, Cook RL, Albaugh TJ, Campoe OC (2018) Advances in silviculture of intensively managed plantations. Curr For Rep 4:23–34. https://doi.org/10.1007/s40725-018-0072-9
Ryan MG, Law BE (2005) Interpreting, measuring, and modeling soil respiration. Biogeochemistry 73:3–27. https://doi.org/10.1007/s10533-004-5167-7
Sayer EJ, Joseph Wright S, Tanner EVJ, Yavitt JB, Harms KE, Powers JS, Kaspari M, Garcia MN, Turner BL (2012) Variable responses of lowland tropical forest nutrient status to fertilization and litter manipulation. Ecosystems 15:387–400. https://doi.org/10.1007/s10021-011-9516-9
Stiles WAV, Rowe EC, Dennis P (2018) Nitrogen and phosphorus enrichment effects on CO2 and methane fluxes from an upland ecosystem. Sci Total Environ 618:1199–1209. https://doi.org/10.1016/j.scitotenv.2017.09.202
Tang X, Liu S, Zhou G, Zhang D, Zhou C (2006) Soil-atmospheric exchange of CO2, CH4, and N2O in three subtropical forest ecosystems in southern China. Glob Change Biol 12:546–560. https://doi.org/10.1111/j.1365-2486.2006.01109.x
Wang QK, Wang SL, Liu YX (2008) Responses to N and P fertilization in a young Eucalyptus dunnii plantation: microbial properties, enzyme activities and dissolved organic matter. Appl Soil Ecol 40:484–490. https://doi.org/10.1016/j.apsoil.2008.07.003
Wang Y, Wang H, Wang ZL, Zhang W, Guo C, Wen X, Liu Y (2012) Optimizing manual sampling schedule for estimating annual soil CO2 efflux in a young exotic pine plantation in subtropical China. Eur J Soil Biol 52:41–47. https://doi.org/10.1016/j.ejsobi.2012.06.002
Wang C, Lu X, Mori T, Mao Q, Zhou K, Zhou G, Nie Y, Mo J (2018) Responses of soil microbial community to continuous experimental nitrogen additions for 13 years in a nitrogen-rich tropical forest. Soil Biol Biochem 121:103–112. https://doi.org/10.1016/j.soilbio.2018.03.009
Wei X, Blanco JA, Jiang H, Kimmins JP (2012) Effects of nitrogen deposition on carbon sequestration in Chinese fir forest ecosystems. Sci Total Environ 416:351–361. https://doi.org/10.1016/j.scitotenv.2011.11.087
Whitehead D, Boelman NT, Turnbull MH, Griffin KL, Tissue DT, Barbour MM, Hunt JE, Richardson SJ, Peltzer DA (2005) Photosynthesis and reflectance indices for rainforest species in ecosystems undergoing progression and retrogression along a soil fertility chronosequence in New Zealand. Oecologia 144:233–244
Xia J, Wan S (2008) Global response patterns of terrestrial plant species to nitrogen addition. New Phytol 179:428–439. https://doi.org/10.1111/j.1469-8137.2008.02488.x
Yang YS, Guo JF, Chen GS, Xie JS, Gao R, Li Z, Jin Z (2005) Litter production, seasonal pattern and nutrient return in seven natural forests compared with a plantation in southern China. Forestry 78:403–415. https://doi.org/10.1093/forestry/cpi044
Zeng W, Wang W (2015) Combination of nitrogen and phosphorus fertilization enhance ecosystem carbon sequestration in a nitrogen-limited temperate plantation of Northern China. For Ecol Manag 341:59–66. https://doi.org/10.1016/j.foreco.2015.01.004
Acknowledgements
This study was supported by Grants from National Basic Research Program of China (973 Program, 2012CB416903), National Natural Science Foundation of China (Nos. 31500360, 30970484), National Key Technology Research and Development Program of the Ministry of Science and Technology of China (No. 2012BAC11B05-3), and the National Key Research and Development Program of China (2016YFD0800601).
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Wang, J., Li, Q., Fu, X. et al. Mechanisms driving ecosystem carbon sequestration in a Chinese fir plantation: nitrogen versus phosphorus fertilization. Eur J Forest Res 138, 863–873 (2019). https://doi.org/10.1007/s10342-019-01208-z
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DOI: https://doi.org/10.1007/s10342-019-01208-z