Abstract
Meloidogyne incognita is one of the most important plant-parasitic nematodes (PPNs) causing severe crop losses worldwide. Plants have evolved complex defense mechanisms to respond to PPNs attacks. Conversely, PPNs have evolved infection mechanisms that involve the secretion of effector proteins into host plants to suppress immune responses and facilitate parasitism. Therefore, effector genes are attractive targets for the genetic improvement of plant resistance to M. incognita. In this study, we functionally characterized the Minc16803 (Minc3s00746g16803) putative effector gene to evaluate its role during plant-nematode interactions. First, we found that the Minc16803 gene is expressed in all nematode life stages and encodes a protein with an N-terminal signal peptide for secretion, a motif characteristic of effector proteins and with the absence of transmembrane domain. In addition, our data demonstrated that transgenic Arabidopsis thaliana lines overexpressing a Minc16803-dsRNA efficiently downregulated the Minc16803 transcripts in infecting nematodes. Furthermore, transgenic lines were significantly less susceptible to M. incognita compared to wild-type control plants. The number of galls per plant was reduced by up to 84%, while the number of egg masses per plant decreased by up to 93.3%. Moreover, galls and feeding sites in the roots of transgenic lines were smaller than those in the control plants. Histological analysis revealed giant cells without cytoplasm, disordered neighboring cells, and malformed maturing nematodes in transgenic galls. Curiously, numerous hatching ppJ2 juveniles were often observed near the female body within the transgenic root tissues before egg mass extrusion. All findings strongly suggest that Minc16803 gene is a promising target to engineer agricultural crops for M. incognita resistance through host-induced gene silencing.
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The data that support the findings of this study and any material presented in the manuscript are available from the corresponding author upon reasonable request.
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Acknowledgements
The authors acknowledge EMBRAPA, UCB, CNPq, CAPES, INCT PlantStress Biotech, and FAPDF for the scientific and financial support.
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This work was supported by grants from INCT PlantStress Biotech, EMBRAPA, CNPq, CAPES, and FAPDF. MFB is grateful to CNPq for a postdoctoral research fellowship (PDJ 150936/2018-4). ITLT is grateful to CAPES/Cofecub project Sv.922/18 for financial support in the researcher and students exchange program between institutions.
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Moreira, V.J.V., Pinheiro, D.H., Lourenço-Tessutti, I.T. et al. In planta RNAi targeting Meloidogyne incognita Minc16803 gene perturbs nematode parasitism and reduces plant susceptibility. J Pest Sci 97, 411–427 (2024). https://doi.org/10.1007/s10340-023-01623-7
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DOI: https://doi.org/10.1007/s10340-023-01623-7