Abstract
The three triterpenic acids, betulinic acid, oleanolic acid, and ursolic acid, are some of the prominent components with medicinal characteristics in jujube fruit (Ziziphus jujuba mill.) HPLC methods can be used to determine them, but the simultaneous determination of these triterpenic acids with a similar structure is difficult. The classical HPLC method needs more time to achieve separated peaks with a satisfactory resolution, and the procedure is costly. Still, chemometric methods coupled with overlapped HPLC peaks can help to make the process faster with less cost. Here, multilayer perceptron networks were used as a chemometric method coupled with HPLC–UV data. In the MLP–HPLC–UV method, the peak area does not require measurement. Instead, spectral data of the analytes in the heavily overlapped peaks were studied. This method was tested by analyzing the synthetic mixture of triterpenic acids. The standard addition method was applied to observe the effect of interferences and recovery evaluation on the yield value of the triterpenic acids in jujube (Ziziphus jujuba mill.) extract. Consequently, prediction data are more accurate, and good recoveries of analytes were obtained. Using a simple mobile phase, shorter run time without internal standard, and complex gradient elution conditions are the superiority of this method over the classical HPLC method.
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Acknowledgements
The authors greatly thank Dr. Nabavi and Dr. Taheri for support of research work in Danesh Azma novin Bakhtar CO. and Edeh Darou Teb pharmaceutical Co. (Tehran. Iran).
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The authors declare that no funds, grants, or other support were received during the preparation of this manuscript. The authors have no relevant financial or non-financial interests to disclose.
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All authors contributed to the study conception and design. The initial idea of the research and supervision of the research and guidance for conducting the experiments and guidance for drafting the manuscript were done by Dr. AAMS. Preparation of samples, execution of experiments, data collection, and drafting of the manuscript have been done by Mrs. ZK. Data analysis and conclusions were jointly carried out by Dr. Amir Abdolah Mehrdad Sharif, Mrs. ZK and Dr. AA. Dr. AA guided and supervised the calculations and statistical analysis. All authors read and approved the final manuscript.
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Khoshsima, Z., Sharif, A.A.M. & Akrami, A. Simultaneous Determination of Triterpenic Acids in Jujube Extract Using HPLC–UV Coupled with Multilayer Perceptron Networks. Chromatographia 86, 617–625 (2023). https://doi.org/10.1007/s10337-023-04273-0
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DOI: https://doi.org/10.1007/s10337-023-04273-0