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Fast RP-HPLC Method for Analysis of Glucosinolates and Phenolics in Moringa stenopetala Leaf Powder Preparations

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Abstract

Moringa stenopetala (M. stenopetala) leaf powder is extensively used as food, for preparation of nutraceuticals and herbal medicines in many developing countries. Glucosinolates and phenolics are known to be important bioactive constituents of these preparations. In this study, a selective and fast RP-HPLC method was developed and validated for simultaneous determination of intact glucosinolates and phenolics in M. stenopetala leaf powders. The developed method was linear with a coefficient of determination (r2) ≥ 0.998, precision ≤ 1.5%, and recovery values close to 100%. The method was also sensitive with detection and quantification limits below 1.5 µg/mL and 5 µg/mL, respectively. Besides the use of external standards, quantitative analysis of multicomponents by a single marker (QAMS) was developed and applied to commercial Moringa samples. The correlation coefficient (r ≥ 0.9993) between the QAMS and the external standard method proved the consistency and the strength of the relationship between the two methods. Rutin was used as single marker for the determination of five bioactive components in M. stenopetala leaf samples. Furthermore, a radar plot was used to differentiate samples collected from different geographical origin. Analysis results of the commercial samples revealed that rutin was found to be the constituent with the highest concentration (varying from 6.6 mg/g to 18.8 mg/g) among the phenolics while glucomoringin (0.2–4.2 mg/g) was the most common glucosinolate.

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Data Availability

All data used to support the findings of this study are available from the corresponding author upon request.

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Funding

G. Sibhat gratefully thanks KU Leuven for his scholarship.

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Authors and Affiliations

  1. Department of Pharmaceutical and Pharmacological Sciences, Pharmaceutical Analysis, KU Leuven, Herestraat 49, O & N2, PB 923, 3000, Leuven, Belgium

    Gereziher Sibhat, Ann Van Schepdael & Erwin Adams

  2. College of Health Sciences, School of Pharmacy, Mekelle University, P.O. Box 1871, Mekelle, Ethiopia

    Gereziher Sibhat & Getu Kahsay

Authors
  1. Gereziher Sibhat
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  2. Getu Kahsay
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  3. Ann Van Schepdael
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  4. Erwin Adams
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Contributions

All authors contributed to the study conception and design. G. Sibhat carried out the experimental work, data analysis, and drafting the paper while G. Kahsay, A. Van Schepdael, and E. Adams were involved in supervising the experimental work, data analysis, resources, and reviewing the paper. Authorship confirmation: All authors have made a significant contribution to the research in the manuscript, approved its claims, agreed to be an author, and meet the ICMJE criteria.

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Correspondence to Getu Kahsay.

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Sibhat, G., Kahsay, G., Van Schepdael, A. et al. Fast RP-HPLC Method for Analysis of Glucosinolates and Phenolics in Moringa stenopetala Leaf Powder Preparations. Chromatographia 86, 425–435 (2023). https://doi.org/10.1007/s10337-023-04256-1

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