Abstract
The vascular endothelial growth factor receptor-2 (VEGFR-2) in some tumor cells is a significant target for drug discovery. In this work, a modified model of VEGFR-2 cell membrane stationary phase (CMSP) was prepared by immobilizing U251 cell membrane onto the surface of chitosan-silica (CTS-SiO2) hybrid carrier. The surface and chromatographic characteristics of VEGFR-2 CMSP were studied. We have developed modified VEGFR-2 cell membrane chromatography for screening drugs and sunitinib malate was used as a positive control. The interaction between the new compounds and membrane receptor was determined by the capacity factors (kʹ). The in vitro cytotoxicity of 10 new compounds on U251 cell viability was determined by MTT test separately to verify the potential pharmacological activity. The modified VEGFR-2 cell membrane chromatographic system demonstrated fast and effective characteristics for screening leading compounds.
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Acknowledgments
This work was supported by Province Natural Science Foundation of Shandong (Grant No. 2009ZRB02230).
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Y. Wang and S. Fang contributed equally to this work.
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Wang, Y., Fang, S., Zhao, G. et al. Preparation and Application of Modified VEGFR-2 Cell Membrane Chromatographic Separation System. Chromatographia 79, 675–684 (2016). https://doi.org/10.1007/s10337-016-3081-5
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DOI: https://doi.org/10.1007/s10337-016-3081-5