Simultaneous Determination of Chloramphenicol, Ciprofloxacin, Nitrofuran Antibiotics and their Metabolites in Fishery Products by CE

Abstract

Chloramphenicol (CAP), ciprofloxacin (CIP), nitrofuran antibiotics (NFA) and their metabolites in fishery products were determined simultaneously by a simple method with capillary electrophoresis. The optimal buffer consisted of 20 mmol L⁻¹ sodium dihydrogen phosphate, 20 mmol L⁻¹ disodium hydrogen phosphate, 80 mmol L⁻¹ sodium deoxycholate, and 10 % methanol (v/v), pH 9.0. Under the best conditions, all analytes were separated completely. The method presented good linearity (r ≥ 0.9997) for CAP, CIP and NFA in the range of 5–100 μg mL⁻¹, and for nitrofuran metabolites in the range of 1–25 μg mL⁻¹. The limit of detection for each compound was estimated as 0.3–1.5 μg kg⁻¹ (S/N = 3) in fishery products. The recoveries ranged from 81.5 to 114.5 %. Finally, the method was applied to real food samples, but neither antibiotics nor metabolites were found.