Abstract
Supercritical fluid chromatography (SFC) is a powerful separation technique particularly in the area of enantioseparations. With rapid analysis speed, wide polarity compatibility, higher column efficiency and lower cost of the mobile phase, SFC is regarded as a better choice than high-performance liquid chromatography for drug discovery. In the development of separation method, the choice of modifier and/or additive is the key point of optimum separation. However, such screening of SFC is typically time-consuming. In this study, an autoblending protocol was introduced to speed up the modifier and/or additive screening process, which was performed on a separate programmable gradient proportioning system. The protocol prepares mobile phases on the fly to speed up the screening of modifiers and/or additives and reduces the waste of solutions. Furthermore, by switching mobile phase in the same run, separation of different types of compounds could also be achieved. This system was successfully applied to screen modifier–additive combinations of three alkaloids and three polyphenols by switching to two mobile phase conditions, as well as by a ternary additive mobile phase on an SFC system. The proposed protocol allows fast separation method development of SFC, which was proved to be rapid, simple, and reproducible.
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References
Liu Z, Zhao S, Wang R, Yang G (1999) J Chromatogr Sci 37:155–158
Dispas A, Lebrun P, Sassiat P, Ziemons E, Thiébaut D, Vial J, Hubert P (2012) J Chromatogr A 1256:253–260
Abrahamsson V, Rodriguez-Meizoso I, Turner C (2012) J Chromatogr A 1250:63–68
Wenda C, Haghpanah R, Rajendran A, Amanullah M (2011) J Chromatogr A 1218:162–170
Sethi N, Anand A, Jain G, Srinivas KS, Chandrul KK (2010) Chron Young Sci 1:12
Pinkston JD, Wen D, Morand KL, Tirey DA, Stanton DT (2006) Anal Chem 78:7467–7472
Ishibashi M, Ando T, Sakai M, Matsubara A, Uchikata T, Fukusaki E, Bamba T (2012) J Chromatogr A 1266:143–148
Laskar DB, Zeng L, Xu R, Kassel DB (2008) Chirality 20:885–895
Pinkston JD, Marapane SB, Jordan GT, Clair BD (2002) J Am Soc Mass Spectrom 13:1195–1208
Ebinger K, Weller HN, Kiplinger J, Lefebvre P (2011) J Lab Automat 16:241–249
McClain RT, Dudkina A, Barrow J, Hartman G, Welch CJ (2008) J Liq Chromatogr Rel Tech 32:483–499
Zheng J, Pinkston JD, Zoutendam PH, Taylor LT (2006) Anal Chem 78:1535–1545
Ventura M, Murphy B, Goetzinger W (2012) J Chromatogr A 1220:147–155
Hamman C, Schmidt DE, Wong M, Hayes M (2011) J Chromatogr A 1218:7886–7894
Blilie AL, Greibrokk T (1985) Anal Chem 57:2239–2242
Thiébaut D (2012) J Chromatogr A 1252:177–188
Smith RD (1986) J Chromatogr 355:367–373
Berger TA (1991) Deye JF (1990). J Chromatogr Sci 29:26–30
Dispas A, Lebrun P, Sassiat P, Ziemons E, Thiébaut D, Vial J, Hubert P (2012) J Chromatogr A 1256:253–260
Kamangerpour A, Ashraf-Khorassani M, Taylor LT, McNair HM, Chorida L (2002) Chromatographia 55:417–421
Blackwell JA, Stringham RW, Weckwerth JD (1997) Anal Chem 69:409–415
Ventura M, Murphy M, Goetzinger W (2012) J Chromatogr A 1220:147–155
Geiser FO, Yocklovich SG, Lurcott SM, Guthrie JW, Gutherie EJ, Levy EJ (1988) J Chromatogr A 459:173–181
Engelhardt H, Gross A, Mertens R, Petersen M (1989) J Chromatogr A 477:169–183
Taylor LT (2012) J Chromatogr A 1250:196–204
Veuthey JL, Caude M, Rosset R (1989) Chromatographia 27:105–108
Chou FM, Wang WT, Wei GT (2009) J Chromatogr A 1216:3594–3599
Alexander AJ, Hooker TF, Tomasella FP (2012) J Pharm Biomed Anal 70:77–86
Hooper PR, Al-Shemmeri T, Goodwin MJ (2012) Appl Therm Eng 48:32–40
Alexander AJ (2012) Chromatographia 75:1185–1190
Janicot JL, Caude M, Rosset R (1988) J Chromatogr A 437:351–364
Acknowledgments
The authors gratefully acknowledge financial supported by a grant from the National Natural Sciences Foundation of China (no. 21072185 and 21302180), a 2010 Technological Talents Program of the Chinese Academy of Sciences granted to Dr. Y. Zhou.
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Xia, B., Feng, M., Ding, L. et al. Fast Separation Method Development for Supercritical Fluid Chromatography Using an Autoblending Protocol. Chromatographia 77, 783–791 (2014). https://doi.org/10.1007/s10337-014-2684-y
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DOI: https://doi.org/10.1007/s10337-014-2684-y