Abstract
Nacobbus aberrans is an endophytic parasitic nematode that induces root galls, negatively affecting plant growth and development, and decreasing the production of economically important crops. Currently, low environmental impact alternatives are being developed for the control of nematodes, such as biological control agents, to reduce the use of soil disinfectants. Nematophagous fungi are microorganisms that can suppress nematode reproduction due to different mechanisms, such as parasitism of eggs, toxin production and stimulation of plant growth. The aim of this study was to investigate the effect of inoculation with nematophagous fungi on N. aberrans in greenhouse-grown pepper (Capsicum anuumm L.) plants and to analyze the response of the infected plants. Two fungal isolates, Purpureocillium lilacimum and Pleurotus ostreatus were tested. The plants were inoculated with these fungi at the time of transplantation in the presence and absence of N. aberrans. The reproduction factor of the nematode was 23.17; 5.90 and 36.4 for P. lilacinum, P. ostreatus and control, respectively. Both in plants parasitized and not parasitized by nematodes, the fungi increased the content of soluble proteins and photosynthetic pigments. Additionally, a favorable impact on growth parameters was also observed. This beneficial effect was also verified by a lower accumulation of proline and sugars, metabolites used by plants as osmoregulators in stress situations, and a low accumulation of malondialdehyde, a metabolite resulting from oxidative stress. These results show that both fungi are suitable for use in the biocontrol of N. aberrans and as growth promoters in plants.
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Acknowledgements
The authors thank Laura Wahnan (CONICET) for the collaboration in field and laboratory tasks.
Funding
Financial support for this study was provided by PICT 2019–00207 to ANPCyT; the Proyecto de Incentivos a la Investigación (A344) and (A316) of the UNLP, Argentina; the grant provided by the Proyecto de Unidades Ejecutoras and PIP 11220200100527CO (CONICET), Argentina. National University of the Northwest of the Province of Buenos Aires (UNNOBA). EXP-0597/2019 approved by Resolution CS N ° 1623/2019.
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Performed the experiments, analyzed the data and wrote the paper: VFB Supervised the work, review and editing: MFR and MCNS Funding acquisition: MFR and MCNS All authors conceived and designed the experiment, contributed to the measurements, processing of the material and participated in the critical review of the manuscript.
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Bernardo, V.F., Gonzalez, M.A., Garita, S.A. et al. Evaluation of two nematophagous fungi for the control of false root-knot nematode Nacobbus aberrans in pepper crops. J Gen Plant Pathol 89, 339–346 (2023). https://doi.org/10.1007/s10327-023-01145-1
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DOI: https://doi.org/10.1007/s10327-023-01145-1