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Multiple Colletotrichum species cause anthracnose disease on Japanese pickling melon var. Katsura-uri (Cucumis melo var. conomon)

  • Fungal Diseases
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Abstract

Katsura-uri is a variety of Japanese pickling melon (Cucumis melo var. conomon) and traditionally cultivated in the Kyoto area in Japan. The thick flesh and crunchy texture of the fruit are excellent for making Japanese pickles. In 2010, dark brown to black anthracnose spots were found on ripening Katsura-uri fruits after harvest. Four fungal strains (T1, K1, K2 and K3) were isolated from lesions on fruits and identified as four Colletotrichum species (C. orbiculare, C. fructicola, C. aenigma and C. chlorophyti) based on phylogenetic analysis. C. orbiculare K3 isolate caused necrotic lesions on Katsura-uri leaves, whereas C. fructicola K1, C. aenigma K2 and C. chlorophyti T1 isolates caused necrotic lesions on Katsura-uri fruits but not on leaves. Inoculation assays to ripe and immature Katsura-uri fruits suggested that C. chlorophyti causes a postharvest disease; necrotic lesions developed only on ripe fruits. This study showed that multiple Colletotrichum species caused anthracnose on Katsura-uri fruits.

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Acknowledgements

We thank Beth E. Hazen for carefully reading the article and giving valuable suggestions.

Funding

The work was supported by JSPS KAKENHI Grant Numbers 24248009 to YK and 24300253 to YN.

Author information

Author notes

  1. Ken Harata

    Present address: Kyoto Prefectural Collage of Agriculture, Kyoto, 623-0221, Japan

  2. Eun Young Park

    Present address: Department of Food and Nutrition, Gangseo University, Seoul, 07661, Republic of Korea

  3. Kenji Sato

    Present address: Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kyoto, 606-8502, Japan

  4. Dong-Liang Jiang and Ken Harata contributed equally to this work.

Authors and Affiliations

  1. Laboratory of Plant Pathology, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Kyoto, 606-8522, Japan

    Dong-Liang Jiang, Ken Harata & Mami Ogawa

  2. Horticultural Division, Kyoto Prefectural Agriculture, Forestry and Fisheries Technology Center, Kameoka, 621-0806, Japan

    Koji Shirota

  3. Laboratory of Food Science, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Kyoto, 606-8522, Japan

    Azusa Sasaki, Eun Young Park, Kenji Sato & Yasushi Nakamura

  4. Laboratory of Farm Management, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Kyoto, 606-8522, Japan

    Takako Nakamura

  5. Department of Food Science and Biotechnology, Faculty of Agriculture, Kagoshima University, Kagoshima, 890-0065, Japan

    Shigehisa Okamoto

  6. Faculty of Agriculture, Setsunan University, Osaka, 573-0101, Japan

    Yasuyuki Kubo

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Contributions

Conceptualization: DJ, KS, YN and YK; methodology: DJ, KH and YK; resources: KS; formal analysis and investigation: DJ, KH, MO, KS, AS, TN, SO, EYP, YN and YK; writing original draft: DJ, KH and YK; review and editing: HK and YK.

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Correspondence to Yasuyuki Kubo.

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Jiang, DL., Harata, K., Ogawa, M. et al. Multiple Colletotrichum species cause anthracnose disease on Japanese pickling melon var. Katsura-uri (Cucumis melo var. conomon). J Gen Plant Pathol 89, 249–259 (2023). https://doi.org/10.1007/s10327-023-01138-0

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