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Biochemical, physiological, and molecular characterization of Dickeya dianthicola (formerly named Erwinia chrysanthemi) causing potato blackleg disease in Japan

  • Bacterial and Phytoplasma Diseases
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Abstract

The taxonomic assignment of Japanese potato blackleg isolates of Dickeya spp. has not been confirmed after the changes in their former name, Erwinia chrysanthemi. Therefore, we investigated and identified 23 representative isolates of Dickeya spp. from symptomatic stems of potatoes in Japan, with biochemical tests and phylogenetic sequence analysis using recA, dnaX, rpoD, gyrB, and 16S rDNA sequences. Results of our biochemical tests showed that all isolates can be assigned to phenon 5 and biovar 1, which are associated with D. dianthicola. Based on the recA, dnaX, rpoD, gyrB, and 16S rDNA sequences, all isolates are in the same clade with D. dianthicola and were clearly distinguished from D. chrysanthemi, D. dadantii, D. dadantii subsp. dieffenbachiae, D. solani, D. zeae, and D. paradisiaca. Therefore, we conclude that Dickeya spp. isolated from potatoes with blackleg symptoms in Japan are D. dianthicola.

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Acknowledgements

This study was financial supported by the Science and Technology Research Promotion Program for the Agriculture, Forestry, Fisheries, and Food industry (no. 27005C). The authors thank Dr. Yuichi Takikawa (Shizuoka University) for providing P. carotovorum subsp. brasiliense (SUPP30) P. wasabiae (SUPP2667, SUPP2668 and SUPP1422) and Hiroshi Uematsu (Yokohama Plant Protection Station) for providing genomic DNA of D. solani (YPPS1551d, YPPS1552d, YPPS1559d and YPPS1600d). The authors thank Enago (http://ww.enago.jp) for the English language review.

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Authors and Affiliations

  1. Hokkaido Agricultural Research Center, National Agriculture and Food Research Organization (HARC/NARO), Hitsujigaoka, Toyohira-ku, Sapporo, Hokkaido, 062-8555, Japan

    Taketo Fujimoto, Takato Nakayama, Takehiro Ohki, Mitsuru Sayama & Tetsuo Maoka

  2. Tokachi Agricultural Experiment Station, Hokkaido Research Organization, Memuro, Hokkaido, 082-0081, Japan

    Shinji Yasuoka

  3. Hokkaido-Chuo Station, Center for Seeds and Seedlings, NARO, Nishinosato, Kitahiroshima, Hokkaido, 061-1102, Japan

    Yoshiyuki Aono

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  1. Taketo Fujimoto
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  2. Shinji Yasuoka
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  3. Yoshiyuki Aono
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Correspondence to Taketo Fujimoto.

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Fujimoto, T., Yasuoka, S., Aono, Y. et al. Biochemical, physiological, and molecular characterization of Dickeya dianthicola (formerly named Erwinia chrysanthemi) causing potato blackleg disease in Japan. J Gen Plant Pathol 84, 124–136 (2018). https://doi.org/10.1007/s10327-018-0772-9

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