Abstract
A total of 395 single-zoospore isolates of Phytophthora sojae that were obtained from 467 soybean fields in Heilongjiang, China from 2011 to 2015 were identified for pathotypes using differential soybean cultivars with Rps1a, Rps1b, Rps1c, Rps1d, Rps1k, Rps2, Rps3a, Rps3b, Rps3c, Rps4, Rps5, Rps6, Rps7, and Rps8 resistance genes. The results showed that P. sojae was widespread in Heilongjiang, but not evenly distributed. A heavy infestation of P. sojae in eastern Heilongjiang coincided with the serious diseases caused by this pathogen in that region. Among 135 pathotypes were identified, 20 isolates matched races of P. sojae based on published race definitions. To our knowledge, this is the first report of races 2, 8, 23, 25, 26, 29, 33, 39, 42, 43, and 48 in Heilongjiang. Race 1, previously considered as the dominant race in Heilongjiang, only comprised 1.52 % of the isolates in the present study. Virulence frequencies of 395 isolates to 14 Rps genes ranged from 17.85 to 82.41 %. Less than 30 % of the isolates were virulent on cultivars containing Rps genes 1k, 1c, or 3a, which indicated that these Rps genes were more effective than other genes in Heilongjiang. All isolates could defeat more than two Rps genes. Ninety-six percent of the isolates were virulent against more than four Rps genes, which indicated that multi-virulence isolates existed in Heilongjiang. All 223 tested isolates were sensitive to metalaxyl, but EC50 for this fungicide against the pathogen increased 18-fold in the last 20 years. The results of the present study suggest that incorporating Rps genes 1c, 1k, and 3a into soybean cultivars and the use of metalaxyl-coated seeds should provide integrated approach to manage Phytophthora root and stem rot of soybean in Heilongjiang, China.
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References
Abney TS, Melgar JC, Richards TL, Scott DH, Grogan J, Young J (1997) New races of Phytophthora sojae with Rps1-d virulence. Plant Dis 81:653–655
Bernard RL, Smith PE, Kaufmman MJ, Schmitthenenner AF (1957) Inheritance of resistance to Phytophthora root and stem rot in the soybean. Agron J 49:391
Burnham KD, Dorrance AE, Francis DM, Fioritto RJ, St. Martin SK (2003) Rps 8, a new locus in soybean for resistance to Phytophthora sojae. Crop Sci 43:101–105
Canaday CH, Schmitthenner AF (1982) Isolating Phytophthora megasperma f. sp. glycinea from soil with a baiting method that minimizes Pythium contamination. Soil Biol Biochem 14:67–68
Costamilan LM, Clebsch CC, Soares RM, Seixas CDS, Godoy CV, Dorrance AE (2013) Pathogenic diversity of Phytophthora sojae pathotypes from Brazil. Eur J Plant Pathol 135:845–853
Cui L, Yin W, Tang Q, Dong S, Zheng X, Zhang Z, Wang Y (2010) Distribution, pathotypes, and metalaxyl sensitivity of Phytophthora sojae from Heilongjiang and Fujian provinces in China. Plant Dis 94:881–884
Cui L, Li J, Lu J, Yin W, Wang Y (2013) Study on resistance risk of Phytophthora sojae to metalaxyl. J Nanjing Agric Univ 36:47–51
Dorrance AE, McClure SA, de Silva A (2003) Pathogenic diversity of Phytophthora sojae in Ohio soybean fields. Plant Dis 87:139–146
Dorrance AE, Jia H, Abney TS (2004) Evaluation of soybean differentials for their interaction with Phytophthora sojae. Plant Health Progress. doi:10.1094/PHP-2004-0309-01-RS
Dorrance AE, Mills D, Robertson AE, Draper MA, Giesler L, Tenuta A (2007) Phytophthora root and stem rot of soybean. Plant Health Instr. doi:10.1094/PHI-I-2007-0830-07
Doupnik B Jr (1993) Soybean production and disease loss estimates for north central United States from 1989 to 1991. Plant Dis 77:1170–1171
Förster H, Tyler BM, Coffey MD (1994) Phytophthora sojae races have arisen by clonal evolution and by rare outcrosses. Mol Plant Microb Interact 7:780–791
Gordon SG, St. Martin SK, Dorrance AE (2006) Rps8 maps to a resistance gene rich region on soybean molecular linkage group F. Crop Sci 46:168–173
Grau CR, Dorrance AE, Bond J, Russin JS (2004) Fungal diseases. Chapter 14. In: Boerma HR, Specht JE (eds) Soybeans: improvement, production, and uses, 3rd edn. no. 16. Amer Soci Agron, Madison, WI
Haas JH, Buzzell RI (1976) New race 5 and 6 of Phytophthora megasperma var. sojae and differential reactions of soybean cultivars for races 1 to 6. Phytopathology 66:1361–1362
Han X, He Z, Zhang Z (1998) The prevention technique of main disease and pests of soybean. Soybean Bull 6:5–6
Henry RN, Kirkpatrick TL (1995) Two new race of Phytophthora sojae, causal agent of Phytophthora root and stem rot of soybean, identified from Arkansas soybean fields. Plant Dis 79:1074
Jackson TA, Kirkpatrick TL, Rupe JC (2004) Races of Phytophthora sojae in Arkansas soybean fields and their effects on commonly grown soybean cultivars. Plant Dis 88:345–351
Kaitany RC, Hart LP, Safir GR (2001) Virulence composition of Phytophthora sojae in Michigan. Plant Dis 85:1103–1106
Kaufmann MJ, Gerdemann JW (1958) Root and stem rot of soybeans caused by Phytophthora sojae n. sp. Phytopathology 48:201–208
Keeling BL (1980) Research on Phytophthora root and stem rot: isolation, testing procedures, and seven new physiologic races. In: Corbin FT (ed) World soybean research conference II: proceedings (General papers), pp 367–370
Keeling BL (1982) Four new physiologic races of Phytophthora megasperma f. sp. glycinea. Plant Dis 66:334–335
Keeling BL (1984) A new physiologic race of Phytophthora megasperma f. sp. glycinea. Plant Dis 68:626–627
Laviolette FA, Athow KL (1977) Three new physiologic races of Phytophthora megasperma var. sojae. Phytopathology 67:267–268
Laviolette FA, Athow KL (1983) Two new physiologic races of Phytophthora megasperma f. sp. glycinea. Plant Dis 67:497–498
Layton AC, Athow KL, Laviolette FA (1986) New physiologic race of Phytophthora megasperma f. sp. glycinea. Plant Dis 70:500–501
Leitz RA, Hartman GL, Pedersen WL, Nickell CD (2000) Races of Phytophthora sojae on soybean in Illinois. Plant Dis 84:487
Li S, Wen J (2012) Diversity of Phytophthora sojae in soil of Heilongjiang Province. Chin J Oil Crop Sci 34:425–432
Li B, Peng Y, Shen C (1993) Sensitivity and variability of Phytophthora megasperma f. sp. glycinea to metalaxyl. Acta Phytopathol Sin 25:23–27
Lv H, Kong F, Xu X, Yuan X, Yang Q (2001) Response of soybean germplasm from the Northeast China to Phytophthora root rot caused by Phytophthora sojae. Chin J Oil Crop Sci 23:16–18
Ma S, Li B (1999) A preliminary report on the identification of the physiological races of Phytophthora megasperma. Soybean Sci 18:151–153
Ma S, Ding J, Zheng T, Gu X (2005) The identification of physiological races of Phytophthora megasperma. Soybean Sci 24:260–262
Meng XQ, Shoemaker RC, Yang XB (1999) Analysis of pathogenicity and genetic variation among Phytophthora sojae isolates using RAPD. Mycol Res 103:173–178
Morgan FL, Hartwig EE (1965) Physiologic specialization in Phytophthora megasperma var. sojae. Phytopathology 55:1277–1279
Nelson BD, Mallik I, McEwen D, Christianson T (2008) Pathotypes, distribution, and metalaxyl sensivity of Phytophthora sojae from North Dakota. Plant Dis 92:1062–1066
Robertson AE, Yang X-B (2004) Update of Phytophthora races present in Iowa. ICM News 492:74
Robertson AE, Cianzio SR, Cerra SM, Pope RO (2009) Within-field pathogenic diversity of Phytophthora sojae in commercial soybean fields in Iowa. Plant Health Progress. doi:10.1094/PHP-2009-0908-01-RS
Ryley MJ, Obst NR, Irwin JAG, Drenth A (1998) Changes in the racial composition of Phytophthora sojae in Australia between 1979 and 1996. Plant Dis 82:1048–1054
Schmitthenner AF (1972) Evidence for a new race of Phytophthora megasperma var. sojae pathogenic to soybean. Plant Dis Rep 56:536–539
Schmitthenner AF (1985) Problems and progress in control of Phytophthora root rot of soybean. Plant Dis 69:362–368
Schmitthenner AF (1999) Phytophthora rot of soybean. In: Hartman GL, Sinclair JB, Rupe JC (eds) Compendium of soybean diseases, 4th edn. American Phytopathological Society Press, St. Paul, MN, pp 39–42
Schmitthenner AF, Hobe M, Bhat RG (1994) Phytophthora sojae races in Ohio over a 10-year interval. Plant Dis 78:269–276
Schwenk FW, Sim T (1974) Race 4 of Phytophthora megasperma var. sojae from soybean proposed. Plant Dis 58:352–354
Shen C, Su Y (1991) Discovery and preliminary studies of Phytophthora megasperma on soybean in China. Acta Phytopathol Sin 21:298
Taylor RJ, Pasche JS, Gudmestad NC (2006) Biological significance of mefenoxam resistance in Phytophthora erythroseptica and its implications for the management of pink rot of potato. Plant Dis 90:927–934
Wagner RE, Wilkinson HT (1992) A new physiological race of Phytophthora sojae on soybean. Plant Dis 76:212
Wang X, Schmitthenner AF, Ma S (1998) Investigations and pathogen isolation of Phytophthora root rot of soybean in Heilongjiang Province. Plant Protect 24:9–11
Wen J, Chen H (2002) Races and virulence phenotypes of Phytophthora sojae in Heilongjiang Province and Mongolia Region of China. Chin J Oil Crop Sci 24:63–66
Wen J, Zhang M (1998) The pathogen causing Phytophthora disease of soybean. Chin J Oil Crop Sci 20:76–78
White DM, Partridge JE, Williams JH (1983) Races of Phytophthora megasperma f. sp. glycinea on soybeans in eastern Nebraska. Plant Dis 67:1281–1282
Wrather JA, Koenning SR (2006) Estimates of disease effects on soybean yields in the United States 2003 to 2005. J Nematol 38:173–180
Wrather JA, Chambers AY, Fox JA, Moore WF, Sciumbato GL (1995) Soybean disease loss estimates for the southern United States, 1974 to 1994. Plant Dis 79:1076–1079
Xu X, Lv H, Qu J, Yang Q (2002) Isolation method for Phytophthora sojae and investigation for the infected regions. Soybean Sci 21:147–150
Xu X, Lv H, Qu J, Yang Q (2003) Phytophthora sojae races in Northeast of China and the virulence evaluation of the isolates. Acta Phytophyl Sin 30:125–128
Yang XB, Ruff RL, Meng XQ, Worknef F (1996) Races of Phytophthora sojae in Iowa soybean fields. Plant Dis 80:1418–1420
Zhang S, Wu J, Xu P, Li W, Zuo Y, Qiu L, Chang R, Chen C, Wang J, Yu A, Jin L (2008) Identification of virulence Phytophthora sojae in Heilongjiang Province and the first report on race 15 in China. Chin J Oil Crop Sci 30:229–234
Zhang S, Xu P, Wu J, Xue A, Zhang J, Li W, Chen C, Chen W, Lv H (2010) Races of Phytophthora sojae and their virulences on soybean cultivars in Heilongjiang, China. Plant Dis 94:87–91
Zhu Z, Wang X, Chang R, Ma S, Wu X, Tian Y (2000) Identification of race of Phytophthora sojae and reaction of soybean germplasm resources in Heilongjiang Province. Sci Agricult Sin 33:62–67
Zuo Y, Hou J, Kang Z, Chen C, Huang L (2005) Studies on resistance of Phytophthora sojae to metalaxyl. Mycosystema 24:422–428
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This research was supported by the Special Fund for Agro-scientific Research in the Public Interest (201303018) and the National Natural Science Foundation of China (Grant No. 31370449).
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Tian, M., Zhao, L., Li, S. et al. Pathotypes and metalaxyl sensitivity of Phytophthora sojae and their distribution in Heilongjiang, China 2011–2015. J Gen Plant Pathol 82, 132–141 (2016). https://doi.org/10.1007/s10327-016-0654-y
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DOI: https://doi.org/10.1007/s10327-016-0654-y