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Transcriptomic responses involved in enhanced production of hypocrellin A by addition of Triton X-100 in submerged cultures of Shiraia bambusicola

  • Fermentation, Cell Culture and Bioengineering - Original Paper
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

The addition of surfactant is a useful strategy to enhance the product yield in submerged fermentation process. In this study, we sought to explore the mechanism for the elicitation of Triton X-100 on production of hypocrellin A (HA) in cultures of Shiraia bambusicola through transcriptomic analysis. Triton X-100 at 2.5% (w/v) not only induced HA biosynthesis in mycelia, but also stimulated the release of HA into the medium. We found 23 of 2463 transcripts, possible candidate genes for HA biosynthesis under Triton X-100 induction. Gene ontology (GO) analysis showed Triton X-100 treatment changed expression of genes involved in transmembrane transport and oxidation–reduction process, indicating that enhanced HA production was mainly due to both elicited biosynthesis in mycelium and the increased membrane permeability for HA release. These data provided new insights into elicitation of surfactants in submerged cultures of fungi.

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Acknowledgements

We are grateful to Y. W. Xiang and Q. D. Zu (OE Biotechnology Co., Ltd., Shanghai, China) for assistance with RNA-Seq analysis.

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  1. College of Pharmaceutical Sciences, Soochow University, 215123, Suzhou, China

    Xiu Yun Lei, Ming Ye Zhang, Yan Jun Ma & Jian Wen Wang

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Correspondence to Jian Wen Wang.

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This work has been supported by the National Natural Science Foundation of China (No. 81473183), Soochow Scholar Program (No. 14317363) and the Postgraduate Research & Practice Innovation Program of Jiangsu Province.

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Lei, X.Y., Zhang, M.Y., Ma, Y.J. et al. Transcriptomic responses involved in enhanced production of hypocrellin A by addition of Triton X-100 in submerged cultures of Shiraia bambusicola . J Ind Microbiol Biotechnol 44, 1415–1429 (2017). https://doi.org/10.1007/s10295-017-1965-5

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