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Characterization of a mutant glucose isomerase from Thermoanaerobacterium saccharolyticum

  • Genetics and Molecular Biology of Industrial Organisms
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

A series of site-directed mutant glucose isomerase at tryptophan 139 from Thermoanaerobacterium saccharolyticum strain B6A were purified to gel electrophoretic homogeneity, and the biochemical properties were determined. W139F mutation is the most efficient mutant derivative with a tenfold increase in its catalytic efficiency toward glucose compared with the native GI. With a maximal activity at 80 °C of 59.58 U/mg on glucose, this mutant derivative is the most active type ever reported. The enzyme activity was maximal at 90 °C and like other glucose isomerase, this mutant enzyme required Co2+ or Mg2+ for enzyme activity and thermal stability (stable for 20 h at 80 °C in the absence of substrate). Its optimum pH was around 7.0, and it had 86 % of its maximum activity at pH 6.0 incubated for 12 h at 60 °C. This enzyme was determined as thermostable and weak-acid stable. These findings indicated that the mutant GI W139F from T. saccharolyticum strain B6A is appropriate for use as a potential candidate for high-fructose corn syrup producing enzyme.

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Authors and Affiliations

  1. College of Life Science, Jiaying University, Meizhou, 514015, People’s Republic of China

    Heng Xu, Zhi-Wei Liu & Qi-He Yang

  2. Bioengineering Center at Xiaoshan, Yangtze Delta Region Institute of Tsinghua University, Hangzhou, 311231, People’s Republic of China

    Dong Shen & Xue-Qiang Wu

Authors
  1. Heng Xu
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  2. Dong Shen
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  3. Xue-Qiang Wu
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  4. Zhi-Wei Liu
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  5. Qi-He Yang
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Corresponding author

Correspondence to Xue-Qiang Wu.

Additional information

H. Xu and D. Shen equally contributed to this study.

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Xu, H., Shen, D., Wu, XQ. et al. Characterization of a mutant glucose isomerase from Thermoanaerobacterium saccharolyticum . J Ind Microbiol Biotechnol 41, 1581–1589 (2014). https://doi.org/10.1007/s10295-014-1478-4

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  • DOI: https://doi.org/10.1007/s10295-014-1478-4

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