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Effects of different pulp-capping materials on cell death signaling pathways of lipoteichoic acid-stimulated human dental pulp stem cells

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Abstract

Purpose

This study aimed to evaluate the response of dental pulp stem cells (DPSCs) cultured with and without lipoteichoic acid (LTA) to different pulp-capping materials. Methods: The cells were cultured and seeded in 6-well plates and exposed to 1% LTA solution. Dycal, ProRoot MTA and Biodentine materials were applied on cells and all groups were evaluated by cell proliferation, viability, cell cycle and cell death signaling pathways for 24 and 72 h.

Results

LTA + Dycal treatment significantly inhibited the proliferation of DPSCs and increased the apoptosis rate of cells more than the other groups at 72 h. Compared to other groups, LTA + Dycal treatment significantly increased the levels of Caspase-3 and AKT and decreased the levels of p-AKT.

Conclusions

The results of this study revealed that all tested materials caused apoptosis in DPSCs via an extrinsic apoptotic pathway. The DPSCs showed an early apoptosis response to the Dycal and a late apoptosis response to the ProRoot MTA and Biodentine treatments. LTA led autophagy and inhibited the proliferation of DPSCs. ProRoot MTA and Biodentin eliminated the LTA's bioactivity with higher efficiency than Dycal.

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Acknowledgements

This paper is a revised and expanded version of a paper entitled ‘Effect of calcium-silicate cements on Lipoteichoic acid-infected and healthy DPSCs’ presented at the AIC 19 th International Congress and CONSEURO, Bologna, Italy, 11-13 May 2017.

Funding

This study was supported by Turkish Scientific and Technological Research Council-TUBITAK [Project Number: 1001-115S431] and Research Fund of Istanbul University [THZ: 2016-20628, BEK: 2017-25051].

Author information

Authors and Affiliations

  1. Department of Paediatric Dentistry, Faculty of Dentistry, University of Istanbul, Istanbul, Turkey

    Sinem Kuru

  2. Department of Paediatric Dentistry, Faculty of Dentistry, University of Istanbul Kent, Beyoglu, 34433, Istanbul, Turkey

    Elif Sepet

  3. Department of Histology and Embryology, University of Biruni, Istanbul, Turkey

    Tülay İrez

  4. Department of Immunology, Aziz Sancar Institute of Experimental Medicine, University of Istanbul, Istanbul, Turkey

    Esin Aktaş

  5. Department of Histology and Embryology, Faculty of Medicine, University of Kocaeli, Stem Cell and Gene Treatment Research and Application Center, Izmit, Turkey

    Yusufhan Yazır & Gökhan Duruksu

  6. Varyans Statistical Consultancy, Kadıköy, Istanbul, Turkey

    Ebru Osmanoglu Akyol

  7. Department of Medical Biochemistry, Faculty of Medicine, University of Istanbul Aydın, Istanbul, Turkey

    Mine Ergüven

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  1. Sinem Kuru
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Contributions

ES, ME and SK designed the study. SK, TI, EA, YY, GD and ME performed the experiments. ES and ME wrote the manuscript. EOA performed the statistical analysis. All authors read and approved the final manuscript.

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Correspondence to Elif Sepet.

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Kuru, S., Sepet, E., İrez, T. et al. Effects of different pulp-capping materials on cell death signaling pathways of lipoteichoic acid-stimulated human dental pulp stem cells. Odontology 109, 547–559 (2021). https://doi.org/10.1007/s10266-020-00571-3

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