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Japanese subgingival microbiota in health vs disease and their roles in predicted functions associated with periodontitis

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Abstract

The present study aimed to identify and compare the microbial signatures between periodontally healthy and periodontitis subjects using 454 sequences of 16S rRNA genes. Subgingival plaque samples were collected from ten periodontally healthy subjects and ten matched chronic periodontitis patients. Bacterial DNA was extracted and next-generation sequencing of 16S rRNA genes was performed. The microbial composition differed between healthy subjects and periodontitis patients at all phylogenetic levels. Particularly, 16 species, including Lautropia mirabilis and Neisseria subflava predominated in healthy subjects, whereas nine species, including Porphyromonas gingivalis and Filifactor alocis predominated in periodontitis. UniFrac, a principal coordinate and network analysis, confirmed distinct community profiles in healthy subjects and periodontitis patients. Using predicted function profiling, pathways involved in phenylpropanoid, GPI-anchor biosynthesis, and metabolism of alanine, arginine, aspartate, butanoate, cyanoamino acid, fatty acid, glutamate, methane, proline, and vitamin B6 were significantly over-represented in periodontitis patients. These results highlight the oral microbiota alterations in microbial composition in periodontitis and suggest the genes and metabolic pathways associated with health and periodontitis. Our findings help to further elucidate microbial composition and interactions in health and periodontitis.

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Acknowledgements

This work was supported by the Japan Society for the Promotion of Science KAKENHI; under Grant 26463129 and under Grant 17K11981. We also thank Dr. Walter Meinzer and Dr. Daiki Tanaka for critical reading of the manuscript.

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Authors and Affiliations

  1. Department of Periodontology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo, Tokyo, 113-8549, Japan

    Eri Ikeda, Takahiko Shiba, Yuichi Ikeda, Akinori Nakasato, Yasuo Takeuchi & Yuichi Izumi

  2. RIKEN Center for Integrative Medical Sciences, 1-7-22 Suehiro-cho, Tsurumi, Yokohama, Kanagawa, 230-0045, Japan

    Wataru Suda & Masahira Hattori

  3. Department of Microbiology and Immunology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku, Tokyo, 160-0016, Japan

    Wataru Suda

  4. Department of Molecular Immunology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo, Tokyo, 113-8549, Japan

    Miyuki Azuma

  5. Faculty of Science and Engineering, Graduate School of Advanced Science and Engineering, Waseda University, 3-4-1 Okubo, Shinjuku, Tokyo, 169-8555, Japan

    Masahira Hattori

  6. Oral Care Perio Center, Southern TOHOKU General Hospital, Southern TOHOKU Research Institute for Neuroscience, 7-115 Yatsuyamada, Koriyama, Fukushima, 963-8052, Japan

    Yuichi Izumi

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  1. Eri Ikeda
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Ikeda, E., Shiba, T., Ikeda, Y. et al. Japanese subgingival microbiota in health vs disease and their roles in predicted functions associated with periodontitis. Odontology 108, 280–291 (2020). https://doi.org/10.1007/s10266-019-00452-4

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