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Remineralization of mechanical loaded resin–dentin interface: a transitional and synchronized multistep process

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Abstract

This study evaluated the ability of different in vitro mechanical loading tests to promote new mineral formation at bonded dentin interfaces. This research demonstrated a sequential transition in the dentin remineralizing procedure through the analysis of the mineral and matrix gradients. Mechanical loading in phosphoric acid (PA)-treated samples promoted a generalized increases in relative presence of minerals, crystallinity, ratio of phosphate peaks and a decrease in the gradient of mineral content. The organic component showed, in general terms, an increase in crosslinking. \(\upalpha \)-helices incremented in sine and square waveform loading. In EDTA + SB specimens, the relative mineral concentration incremented when loading in hold, in general. Nonuniform parameters of Bis-GMA and adhesive penetration were encountered in both groups. PA + SB promoted the highest dentin mineralization degree when loading in square, based on the increase in the relative presence of minerals and crystallinity. EDTA + SB produced any advance crystallographic maturity at the interface. High crosslinking parameters and conformational changes in proteins in PA-treated specimens indicated, indirectly, that the first remineralization is intrafibrillar.

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Acknowledgments

This work was supported by Grants CICYT/ FEDER MAT2011-24551, JA-P08-CTS-3944 and CEI-Biotic UGR. The authors have no financial affiliation or involvement with any commercial organization with direct financial interest in the materials discussed in this manuscript. Any other potential conflict of interest is disclosed.

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Authors and Affiliations

  1. Faculty of Dentistry, Dental Materials Section, University of Granada, Colegio Máximo de Cartuja s/n, 18071 , Granada, Spain

    Manuel Toledano, Fátima S. Aguilera, Inmaculada Cabello & Raquel Osorio

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  1. Manuel Toledano
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  2. Fátima S. Aguilera
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  3. Inmaculada Cabello
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  4. Raquel Osorio
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Correspondence to Manuel Toledano.

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Toledano, M., Aguilera, F.S., Cabello, I. et al. Remineralization of mechanical loaded resin–dentin interface: a transitional and synchronized multistep process. Biomech Model Mechanobiol 13, 1289–1302 (2014). https://doi.org/10.1007/s10237-014-0573-9

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  • DOI: https://doi.org/10.1007/s10237-014-0573-9

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