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Analysis of Biosorption Parameters, Equilibrium Isotherms and Thermodynamic Studies of Chromium (VI) Uptake by a Nostoc sp. Isolated from a Coal Mining Site in Meghalaya, India

梅加拉亚邦(印度)煤矿分离念珠藻属的铬(VI)生物吸附参数、吸附平衡等温线和热动力研究

Analyse von Biosorptionsparametern, Gleichgewichtsisotherme und thermodynamische Untersuchungen der Chrom (VI)-Aufnahme durch Nostoc sp., isoliert von einer Kohlemine in Meghalaya, Indien

Análisis de parámetros de biosorción, isotermas de equilibrio y estudios termodinámicos de la captación de cromo (VI) por Nostoc sp aislada de un sitio de extracción de carbón en Meghalaya, India

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Abstract

A cyanobacterium isolated from a water sample collected from a coal mine in the West Khasi Hills of Meghalaya, India, and identified as Nostoc sp. (accession no. KX814344) using 16S rRNA analysis showed a high tolerance to chromium. It was shown to be able to grow in the presence of 15 ppm Cr, which is 30 times the highest Cr concentration recorded in the area. Cr biosorption by the cyanobacterium was optimum at pH 6.0 with 3 µg mL−1 biomass. The sorption showed a linear correlation with increasing metal concentration, gradually reaching saturation. An energy dispersive X-ray study verified Cr binding on the cyanobacterial biomass, and FTIR analysis revealed many negatively charged functional groups on the cell surface, which aided in metal binding. Thermodynamic studies showed the biosorption process to be energetically favorable: − 0.479, − 0.665, and − 0.852 kJ mol−1 at temperatures of 293, 303, and 313K, respectively. Sorption isotherm data fit the Langmuir isotherm best, indicating the monolayer nature of the Cr sorption. The organism’s maximum sorption capacity was as high as 20 mg of Cr per g of biomass. The separation factor calculated from the Langmuir isotherm was < 1, signifying favorable interaction between the cyanobacterial biomass and the Cr ions.

抽象

蓝绿藻提取于印度梅加拉亚邦西卡希山(West Khasi Hills)的煤矿水样,经16S rRNA测序鉴定为Nostoc sp.(登记号:KX814344),蓝绿藻表现出很高的耐铬性,可以在铬浓度高于环境30倍的15ppm条件下生存。蓝绿藻的铬生物吸附最佳条件为pH值为6和生物量为3 µg mL-1。吸附能力随金属浓度增大线性提高,逐渐达吸附饱和。能谱分析证实铬确实结合于蓝绿藻群落,傅里叶转换红外光谱(FTIR)分析表明蓝绿藻细胞表面带很多有助于吸附金属的负电荷功能团。热力学研究表明,温度293、303和313K条件下,生物吸附过程有利条件分别为-0.479kJ mol-1、-0.665 kJ mol-1和-0.852 kJ mol-1。吸附数据服从朗缪尔吸附等温线,属于单层铬吸附。每克生物的最大铬生物吸附能力为20 mg (20 mg of Cr per g of biomass)。由朗缪尔吸附等温线计算的分离系数小于1,蓝绿藻与铬离子利于反应。

Zusammenfassung

Aus einer Wasserprobe, entnommen in einer Kohlemine in den West Khasi Hills von Meghalaya (Indien), wurde ein Cyanobakterium isoliert und unter Verwendung der 16S-rRNA-Analyse als Nostoc sp. (GenBank-Zugangs¬nummer KX814344) identifiziert. Dieser Organismus zeigte eine hohe Toleranz gegenüber Chrom. Es wurde gezeigt, dass er in der Lage ist in Gegenwart von 15 ppm Cr zu wachsen, was dem 30-fachen der in diesem Bereich aufgezeichneten Höchstkonzentration an Cr entspricht. Die Cr-Biosorption durch das Cyanobakterium war mit 3 μg mL-1 Biomasse bei pH 6,0 optimal. Die Sorption zeigte eine lineare Korrelation mit steigender Metallkonzentration und erreichte allmählich die Sättigung. Eine energiedispersive Röntgenuntersuchung bestätigte die Cr-Bindung an der cyanobakteriellen Biomasse, und eine FTIR-Analyse zeigte viele negativ geladene funktionelle Gruppen an der Zelloberfläche, die die Metallbindung unterstützten. Thermodynamische Studien veranschaulichten, dass der Biosorptionsprozess energetisch begünstig ist: -0,479 kJ mol-1, -0,665 kJ mol-1 und -0,852 kJ mol-1 bei Temperaturen von 293, 303 bzw. 313 K. Sorptionsisothermen-Daten wurden am besten durch die Langmuir-Isotherme angepasst, was auf den Charakter einer Einzelschicht bei der Cr-Sorption hindeutet. Die maximale Sorptionskapazität des Organismus lag bei 20 mg Cr pro g Biomasse. Der aus der Langmuir-Isotherme berechnete Trennfaktor betrug <1, was eine günstige Wechselwirkung zwischen der cyanobakteriellen Biomasse und den Cr-Ionen anzeigt.

Resumen

Una cianobacteria aislada de una muestra de agua recogida de una mina de carbón en las colinas West Khasi de Meghalaya, India, e identificada como Nostoc sp. (número de acceso KX814344) usando análisis 16S rRNA mostró una alta tolerancia a cromo. Se demostró que podía crecer en presencia de 15 ppm de Cr, que es 30 veces la concentración de Cr más alta registrada en el área. La biosorción de Cr por la cianobacteria fue óptima a pH 6,0 con 3 μg mL-1 de biomasa. La sorción mostró una correlación lineal con el aumento de la concentración de metal, alcanzando gradualmente la saturación. Mediante un estudio de rayos X de energía dispersiva se verificó la unión de Cr en la biomasa de cianobacterias y el análisis FTIR reveló muchos grupos funcionales cargados negativamente en la superficie de la célula que contribuyeron a la unión del metal. Los estudios termodinámicos mostraron que el proceso de biosorción era energéticamente favorable: -0,479 kJ mol-1, -0,665 kJ mol-1 y -0,852 kJ mol-1 a 293, 303 y 313 K, respectivamente. Los datos de isotermas de sorción se ajustan a la isoterma de Langmuir indicando la naturaleza monocapa de la adsorción de Cr. La capacidad de sorción máxima del organismo fue 20 mg Cr/ g de biomasa. El factor de separación calculado a partir de la isoterma de Langmuir fue <1, lo que significa una interacción favorable entre la biomasa cianobacteriana y los iones Cr.

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Acknowledgements

The authors acknowledge the University Grants Commission, New Delhi, Government of India for financial assistance and granting the National Fellowship for Higher Education of ST students. The authors also acknowledge the Department of Chemistry for FTIR facility and SAIF, North Eastern Hill University for GF-AAS and SEM services.

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  1. Department of Biochemistry, North-Eastern Hill University, Shillong, 793022, India

    Susan Moore Warjri & Mayashree B. Syiem

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  1. Susan Moore Warjri
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Correspondence to Mayashree B. Syiem.

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Warjri, S.M., Syiem, M.B. Analysis of Biosorption Parameters, Equilibrium Isotherms and Thermodynamic Studies of Chromium (VI) Uptake by a Nostoc sp. Isolated from a Coal Mining Site in Meghalaya, India. Mine Water Environ 37, 713–723 (2018). https://doi.org/10.1007/s10230-018-0523-3

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