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Genetic population structure of the fluvial eight-barbel loach Lefua sp. 1 in the three river systems in central Honshu, Japan, revealed by microsatellite DNA markers

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Abstract

The fluvial eight-barbel loach Lefua sp. 1 is an undescribed species distributed from the Kinki to Chugoku districts, Honshu, and also on Shikoku Island, Japan. Genetic relationships among local populations are unclear and management units remain undetermined. To aid conservation, we determined genetic population structures from microsatellite loci for 20 populations from three river systems on Honshu. The genetic diversity within populations is relatively low; the majority has experienced genetic bottlenecks. Statistical analysis revealed significant divergence among river systems suggesting that each should be recognized as a management unit. Any conservation program should consider the populations’ genetic uniqueness.

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Acknowledgments

We are grateful to Toshiya Hirowatari of Kyushu University, Kohsuke Akita of Japan Wildlife Research Center, Katsutoshi Watanabe, Yohsuke Kojima, Yo Yamasaki, and Naoyuki Nakahama of Kyoto University, Jun Nakajima of Fukuoka Institute of Health and Environmental Sciences, and Yuichiro Nakayama, Shohei Ueda, Masahiro Suzuki, and Yoshiko Sakamoto of Osaka Prefecture University for their valuable advices. We thank Kazuhiko Uehara and Kazuya Hiramatsu of the Aquatic Life Conservation Research Center, Shuichi Fukuhara of Baika Educational Institution, Nobuyuki Higashiguchi of Suma Aqualife Park, Yuki Taguchi of Asa Zoo, Taiki Ito of Kinki University, Tetsuo Kamei of Zacconekan, and Tatsuya Yokoyama of the Water Museum for providing helpful information about this species. We also thank Ryohei Yamada, Haruka Matsuoka, and the members of the Entomological Laboratory for their help with field surveys.

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Authors and Affiliations

  1. Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Gakuen-cho 1-1, Naka-ku, Sakai, Osaka, 599-8531, Japan

    Akito Inotsuka, Norio Hirai & Minoru Ishii

  2. Tarumi Hygiene Inspection Office, 1-5-1 Hyuga, Tarumi, Kobe, Hyogo, 655-8570, Japan

    Shigeru Aoyama

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  1. Akito Inotsuka
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  2. Norio Hirai
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  3. Shigeru Aoyama
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  4. Minoru Ishii
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Correspondence to Norio Hirai.

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Inotsuka, A., Hirai, N., Aoyama, S. et al. Genetic population structure of the fluvial eight-barbel loach Lefua sp. 1 in the three river systems in central Honshu, Japan, revealed by microsatellite DNA markers. Ichthyol Res 64, 232–239 (2017). https://doi.org/10.1007/s10228-016-0549-0

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  • DOI: https://doi.org/10.1007/s10228-016-0549-0

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