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Phylogeography of Opsariichthys platypus in Japan based on mitochondrial DNA sequences

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Abstract

To investigate the phylogeography of the Japanese pale chub Opsariichthys platypus, we examined the genetic differentiation, phylogenetic relationships, and historical demography using mitochondrial cytochrome b gene sequences of 788 O. platypus from 124 localities throughout the Japanese Archipelago. Molecular phylogenetic analyses revealed that Japanese O. platypus is divided into three major clades (western Japan: WJ; eastern Japan: EJ; and Kyushu: KY), and that among these clades, KY is remarkably differentiated from the others. The distribution of the EJ and KY clades clearly reflects their respective geographical ranges: the EJ and KY clades are restricted to the eastern region of the Ibuki–Suzuka Mountains in central Honshu and northern Kyushu, respectively. In contrast to the EJ and KY clades, the WJ clade is widely distributed throughout the Japanese Archipelago, including areas where O. platypus is not naturally distributed (e.g., northern part of Honshu, southern Kyushu, and Tokunoshima). In addition, nearly all the WJ haplotypes in the non-indigenous regions were the same as or similar to the haplotypes in Lake Biwa, and the WJ haplotypes in the distribution ranges of the EJ and KY clades were also same as or similar to those of Lake Biwa, indicating that the distribution of WJ clade had been the result of inadvertent releases in conjunction with releases of Plecoglossus altivelis from Lake Biwa. The estimated divergence time indicated that each clade was formed during or before the Pleistocene, and mismatch distribution test suggested the occurrence of the population expansion in three clades and the time since expansion was 120,000–226,000 years. This study demonstrates that O. platypus exhibits clear genetic differentiation among regional populations, and that range expansion following divergence caused by uplifting of mountains is important for distribution and genetic structuring of O. platypus. In addition, artificial introductions of non-indigenous populations have proceeded throughout the Japanese Archipelago, indicating ongoing loss of the genetic features due to introductions or introgression. Further efforts should be made toward a comprehensive understanding of the current state of introductions of non-indigenous O. platypus populations and the potential influence of invasive populations, including loss of genetic diversity.

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Acknowledgments

We are grateful to S. Kitano (Nagano Nature Conservation Research Institute), J. Mima (Environmental Assessment Center CO., LTD), T. Yodo (Mie University), K. Hirashima (Wakayama Prefectural Museum of Natural History), T. Tsuruta (Osaka Sangyo University), M. Shibuya (Sumiko Techno Research, Co., Ltd), T. Nomura (Kanagawa Prefecture), T. Kitamura (Akankohan Eco Museum Center), T. Shimizu (Ehime Prefectural Chuyo Fisheries Experimental Station), T. Ohnaka (Aichi Prefecture), T. Abe (Okayama University), K. Sakai (Noto Marine Center), and Y. Koya, C. Sato, Y. Takeuchi, Y. Ono, A. Tanaka, C. Kato, R. Sakai (Gifu University) for their cooperation with the collection of specimens and laboratory experiments. Hiroshi Takahashi and the two anonymous reviewers also provided comments that helped to improve the manuscript. This work was supported by the Global Environment Research Fund (RF-075 and RF-0910) of the Ministry of the Environment, Japan, and by JSPS KAKENHI Grant Numbers 21370035 and 26250044.

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  1. Jun Nakajima

    Present address: Fukuoka Institute of Health and Environmental Sciences, Mukaizano 39, Dazaifu, Fukuoka, 818-0135, Japan

  2. Yoichi Kawaguchi

    Present address: Laboratory of Ecosystem Management, Division of Ecosystem Design, Institute of Technology and Science, The University of Tokushima, 2-1 Minami-Josanjima, Tokushima, 770-8506, Japan

Authors and Affiliations

  1. Faculty of Regional Studies, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan

    Shigeru Kitanishi, Akari Hayakawa & Takahiko Mukai

  2. National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki, 305-8506, Japan

    Kenzi Takamura

  3. Graduate School of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka, 819-0395, Japan

    Jun Nakajima & Yoichi Kawaguchi

  4. Fishery Research Laboratory, Kyushu University, 4-46-24 Tsuyazaki, Fukutsu, Fukuoka, 811-3304, Japan

    Norio Onikura

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  1. Shigeru Kitanishi
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Kitanishi, S., Hayakawa, A., Takamura, K. et al. Phylogeography of Opsariichthys platypus in Japan based on mitochondrial DNA sequences. Ichthyol Res 63, 506–518 (2016). https://doi.org/10.1007/s10228-016-0522-y

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