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miR-339-3p inhibits cell growth and epithelial–mesenchymal transition in nasopharyngeal carcinoma by modulating the KAT6A/TRIM24 axis

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International Journal of Clinical Oncology Aims and scope Submit manuscript

Abstract

Objective

To explore the effect and mechanism of the miR-339-3p/KAT6A/TRIM24 axis in nasopharyngeal carcinoma (NPC) cell growth and epithelial–mesenchymal transition (EMT) progression.

Methods

CNE2 and 5-8F NPC cell lines were transfected with miR-339-3p-mimic or sh-KAT6A alone or co-transfected with miR-339-3p-mimic and oe-KAT6A. The expression levels of miR-339-3p, KAT6A, TRIM24, and EMT-related proteins were assessed, in addition to cell biological behaviors. Then, the relationship between miR-339-3p and KAT6A was predicted and validated. The correlations between miR-339-3p and KAT6A or between KAT6A and TRIM24 were analyzed by Pearson coefficient and the enrichment of H3K23ac in TRIM24 promoter region was measured by chromatin immunoprecipitation.

Results

miR-339-3p was downregulated, but KAT6A and TRIM24 were highly expressed in NPC cells and tissues. Upregulated miR-339-3p or downregulated KAT6A could inhibit the growth and EMT of NPC cells. Further experiments showed that miR-339-3p regulated NPC cell growth and EMT by mediating KAT6A in a targeted fashion. KAT6A was positively correlated with TRIM24, and the enrichment of H3K23ac was much higher in NPC tissues. miR-339-3p suppressed the growth and EMT of NPC cells by the KAT6A/TRIM24 axis. In a xenograft study, miR-339-3p overexpression inhibited NPC tumor growth in vivo.

Conclusion

Conclusively, miR-339-3p inhibited the growth and EMT of NPC cells via the KAT6A/TRIM24 axis.

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Authors and Affiliations

  1. Department of Otolaryngology, Head and Neck Surgery, The First Affiliated Hospital of Zhengzhou University, No. 1, East Jianshe Road, Erqi District, Zhengzhou, Henan, 450052, People’s Republic of China

    Pei Gao, Wuhao Lu, Liang Wang & Peng Zhang

  2. Department of Otology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, 450052, People’s Republic of China

    Kun Zhao

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  1. Pei Gao
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  2. Kun Zhao
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  3. Wuhao Lu
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  4. Liang Wang
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  5. Peng Zhang
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Contributions

PG is the guarantor of integrity of the entire study and contributed to the experimental studies and manuscript preparation. KZ contributed to the experimental studies. Wuhao Lu contributed to the manuscript preparation and statistical analysis. LW contributed to the experimental studies. PZ contributed to the experimental studies and manuscript preparation. KZ contributed to the analysis and interpretation of data. Wu contributed to the literature research. Liang Wang contributed to the data acquisition and literature research. PZ contributed to the statistical analysis. PG drafted the article and revised it critically. All authors read and approved the final manuscript.

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Correspondence to Pei Gao.

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Gao, P., Zhao, K., Lu, W. et al. miR-339-3p inhibits cell growth and epithelial–mesenchymal transition in nasopharyngeal carcinoma by modulating the KAT6A/TRIM24 axis. Int J Clin Oncol 27, 1684–1697 (2022). https://doi.org/10.1007/s10147-022-02231-8

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  • DOI: https://doi.org/10.1007/s10147-022-02231-8

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