Abstract
Thymidine kinase 1 (TK1) level is an independent survival prognostic factor for both premalignant and malignant cervical pathologies. Herein, this study sought to probe the impacts of TK1 on cervical cancer (CC) progression and its underlying mechanism. Transcription factor Dp-1 (TFDP1) and TK1 expression was assessed using qRT-PCR in CC cell lines. After ectopic expression and knockdown experiments, cell counting kit-8 and colony formation assays were adopted to measure cell proliferation, western blot to examine the expression of epithelial-mesenchymal transition (EMT)-related proteins, and Transwell assays to assess cell invasion and migration. The binding of TFDP1 to TK1 was predicted by bioinformatic sites and verified by chromatin immunoprecipitation and dual-luciferase reporter assays. Tumor xenograft experiments in nude mice were performed to validate the influence of TFDP1/TK1 on CC progression in vivo. CC cells had high TK1 and TFDP1 expression. TFDP1 or TK1 knockdown restrained CC cell EMT, invasion, migration, and proliferation. TFDP1 facilitated TK1 expression in CC via transcription. Overexpression of TK1 counteracted the suppressive impacts of TFDP1 knockdown on CC cell malignant behaviors. Moreover, TFDP1 knockdown depressed CC growth in vivo by downregulating TK1. TFDP1 knockdown restricted proliferation and EMT in CC by downregulating TK1 expression.
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The datasets used or analyzed during the current study are available from the corresponding author on reasonable request.
References
Ayuk SM, Abrahamse H, Houreld NN (2016) The role of photobiomodulation on gene expression of cell adhesion molecules in diabetic wounded fibroblasts in vitro. J Photochem Photobiol B 161:368–374. https://doi.org/10.1016/j.jphotobiol.2016.05.027
Bitter EE, Townsend MH, Erickson R, Allen C, O’Neill KL (2020) Thymidine kinase 1 through the ages: a comprehensive review. Cell Biosci 10:138. https://doi.org/10.1186/s13578-020-00493-1
Chen G, He C, Li L, Lin A, Zheng X, He E, Skog S (2013) Nuclear TK1 expression is an independent prognostic factor for survival in pre-malignant and malignant lesions of the cervix. BMC Cancer 13:249. https://doi.org/10.1186/1471-2407-13-249
Chen C, Liu J, Zhou F, Sun J, Li L, Jin C, Shao J, Jiang H, Zhao N, Zheng S, Lin B (2014) Next-generation sequencing of colorectal cancers in Chinese: identification of a recurrent frame-shift and gain-of-function Indel mutation in the TFDP1 gene. OMICS 18:625–635. https://doi.org/10.1089/omi.2014.0058
Dang L, Ma H, Hei A, Xu S, Zhou J, He E, Skog S (2020) A meta-analysis of serological thymidine kinase 1 as a marker for colorectal benign and malignant tumor risk assessment. Mol Clin Oncol 12:440–450. https://doi.org/10.3892/mco.2020.2002
D’Oria O, Corrado G, Lagana AS, Chiantera V, Vizza E, Giannini A (2022) New advances in cervical cancer: from bench to bedside. Int J Environ Res Public Health 19:7094. https://doi.org/10.3390/ijerph19127094
Drucker E, Holzer K, Pusch S, Winkler J, Calvisi DF, Eiteneuer E, Herpel E, Goeppert B, Roessler S, Ori A, Schirmacher P, Breuhahn K, Singer S (2019) Karyopherin alpha2-dependent import of E2F1 and TFDP1 maintains protumorigenic stathmin expression in liver cancer. Cell Commun Signal 17:159. https://doi.org/10.1186/s12964-019-0456-x
Ghosh S, Jayaram P, Kabekkodu SP, Satyamoorthy K (2022) Targeted drug delivery in cervical cancer: current perspectives. Eur J Pharmacol 917:174751. https://doi.org/10.1016/j.ejphar.2022.174751
Hanouskova L, Rezac J, Vesely S, Prusa R, Kotaska K (2020) Thymidine kinase-1 as additional diagnostic marker of prostate cancer. Clin Lab 66:1045–1049. https://doi.org/10.7754/Clin.Lab.2019.191026
He J, Huang B, Zhang K, Liu M, Xu T (2020) Long non-coding RNA in cervical cancer: from biology to therapeutic opportunity. Biomed Pharmacother 127:110209. https://doi.org/10.1016/j.biopha.2020.110209
Jiang ZF, Wang M, Xu JL (2018) Thymidine kinase 1 combined with CEA, CYFRA21-1 and NSE improved its diagnostic value for lung cancer. Life Sci 194:1–6. https://doi.org/10.1016/j.lfs.2017.12.020
Jibrim RLM, de Carvalho CV, Invitti AL, Schor E (2019) Expression of the TFDP1 gene in the endometrium of women with deep infiltrating endometriosis. Gynecol Endocrinol 35:490–493. https://doi.org/10.1080/09513590.2018.1540569
Johnson CA, James D, Marzan A, Armaos M (2019) Cervical cancer: an overview of pathophysiology and management. Semin Oncol Nurs 35:166–174. https://doi.org/10.1016/j.soncn.2019.02.003
Ju H, Tan JY, Cao B, Song MQ, Tian ZB (2018) Effects of miR-223 on colorectal cancer cell proliferation and apoptosis through regulating FoxO3a/BIM. Eur Rev Med Pharmacol Sci 22:3771–3778. https://doi.org/10.26355/eurrev_201806_15259
Li J, Gu C, Zheng H, Geng X, Yang Z, Zhou L, Wu H (2021) Ultrasonographic diagnosis in rare primary cervical cancer. Int J Gynecol Cancer 31:1535–1540. https://doi.org/10.1136/ijgc-2021-002860
Liu C, Wang J, Zhao L, He H, Zhao P, Peng Z, Liu F, Chen J, Wu W, Wang G, Dong F (2019) Knockdown of thymidine kinase 1 suppresses cell proliferation, invasion, migration, and epithelial-mesenchymal transition in thyroid carcinoma cells. Front Oncol 9:1475. https://doi.org/10.3389/fonc.2019.01475
Lu X, Lv XD, Ren YH, Yang WD, Li ZB, Zhang L, Bai XF (2016) Dysregulation of TFDP1 and of the cell cycle pathway in high-grade glioblastoma multiforme: a bioinformatic analysis. Genet Mol Res 15:2. https://doi.org/10.4238/gmr.15027646
Lundgren PO, Tribukait B, Kjellman A, Norming U, Jagarlmudi K, Gustafsson O (2022) Serum thymidine kinase 1 concentration as a predictive biomarker in prostate cancer. Prostate 82:911–916. https://doi.org/10.1002/pros.24335
Malvi P, Janostiak R, Nagarajan A, Cai G, Wajapeyee N (2019) Loss of thymidine kinase 1 inhibits lung cancer growth and metastatic attributes by reducing GDF15 expression. PLoS Genet 15:e1008439. https://doi.org/10.1371/journal.pgen.1008439
Mayadev JS, Ke G, Mahantshetty U, Pereira MD, Tarnawski R, Toita T (2022) Global challenges of radiotherapy for the treatment of locally advanced cervical cancer. Int J Gynecol Cancer 32:436–445. https://doi.org/10.1136/ijgc-2021-003001
McCartney A, Malorni L (2020) Thymidine kinase-1 as a biomarker in breast cancer: estimating prognosis and early recognition of treatment resistance. Biomark Med 14:495–498. https://doi.org/10.2217/bmm-2020-0072
Moscovich M, LeDoux MS, Xiao J, Rampon GL, Vemula SR, Rodriguez RL, Foote KD, Okun MS (2013) Dystonia, facial dysmorphism, intellectual disability and breast cancer associated with a chromosome 13q34 duplication and overexpression of TFDP1: case report. BMC Med Genet 14:70. https://doi.org/10.1186/1471-2350-14-70
Ohdaira H, Sekiguchi M, Miyata K, Sasaki T, Yoshida K (2012) Acute loss of DP1, but not DP2, induces p53 mRNA and augments p21Waf1/Cip1 and senescence. Cell Biochem Funct 30:54–60. https://doi.org/10.1002/cbf.1818
Pang Y, Liu J, Li X, Xiao G, Wang H, Yang G, Li Y, Tang SC, Qin S, Du N, Zhang H, Liu D, Sun X, Ren H (2018) MYC and DNMT3A-mediated DNA methylation represses microRNA-200b in triple negative breast cancer. J Cell Mol Med 22:6262–6274. https://doi.org/10.1111/jcmm.13916
Paoletti C, Barlow WE, Cobain EF, Bergqvist M, Mehta RS, Gralow JR, Hortobagyi GN, Albain KS, Pusztai L, Sharma P, Godwin AK, Thompson AM, Hayes DF, Rae JM (2021) Evaluating serum thymidine kinase 1 in patients with hormone receptor-positive metastatic breast cancer receiving first-line endocrine therapy in the SWOG S0226 trial. Clin Cancer Res 27:6115–6123. https://doi.org/10.1158/1078-0432.CCR-21-1562
Qiu HZ, Huang J, Xiang CC, Li R, Zuo ED, Zhang Y, Shan L, Cheng X (2020) Screening and discovery of new potential biomarkers and small molecule drugs for cervical cancer: a bioinformatics analysis. Technol Cancer Res Treat 19:1533033820980112. https://doi.org/10.1177/1533033820980112
Sung H, Ferlay J, Siegel RL, Laversanne M, Soerjomataram I, Jemal A, Bray F (2021) Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin 71:209–249. https://doi.org/10.3322/caac.21660
Wang Z, Zhang W, Huo B, Dong L, Zhang J (2020) Relationship between thymidine kinase 1 before radiotherapy and prognosis in breast cancer patients with diabetes. Biosci Rep 40:BSR20192813. https://doi.org/10.1042/BSR20192813
Wang L, Sharif H, Saellstrom S, Ronnberg H, Eriksson S (2021) Feline thymidine kinase 1: molecular characterization and evaluation of its serum form as a diagnostic biomarker. BMC Vet Res 17:316. https://doi.org/10.1186/s12917-021-03030-5
Wu X, Fan Y, Liu Y, Shen B, Lu H, Ma H (2021) Long non-coding RNA CCAT2 promotes the development of esophageal squamous cell carcinoma by inhibiting miR-200b to upregulate the IGF2BP2/TK1 axis. Front Oncol 11:680642. https://doi.org/10.3389/fonc.2021.680642
Yang Q, Al-Hendy A (2022) The regulatory functions and the mechanisms of long non-coding RNAs in cervical cancer. Cells 11:1149. https://doi.org/10.3390/cells11071149
Yang L, Cui Y, Huang T, Sun X, Wang Y (2020) Identification and validation of MSX1 as a key candidate for progestin resistance in endometrial cancer. Onco Targets Ther 13:11669–11688. https://doi.org/10.2147/OTT.S271494
Yu G, Wang J, Lin X, Diao S, Cao Y, Dong R, Wang L, Wang S, Fan Z (2016) Demethylation of SFRP2 by histone demethylase KDM2A regulated osteo-/dentinogenic differentiation of stem cells of the apical papilla. Cell Prolif 49:330–340. https://doi.org/10.1111/cpr.12256
Zhang L, Li H, Qiu Y, Liu Y, Liu X, Wang W (2021) Screening and cellular validation of prognostic genes regulated by super enhancers in oral squamous cell carcinoma. Bioengineered 12:10073–10088. https://doi.org/10.1080/21655979.2021.1997089
Zhou J, He E, Skog S (2013) The proliferation marker thymidine kinase 1 in clinical use. Mol Clin Oncol 1:18–28. https://doi.org/10.3892/mco.2012.19
Zhou J, Li H, Fang C, Gao P, Jin C, Liu S, Zou R, Li J, Liu Y, He E, Skog S (2022) Concentration of human thymidine kinase 1 discover invisible malignant human tumours. Eur J Cell Biol 101:151280. https://doi.org/10.1016/j.ejcb.2022.151280
Zhu X, Shi C, Peng Y, Yin L, Tu M, Chen Q, Hou C, Li Q, Miao Y (2018) Thymidine kinase 1 silencing retards proliferative activity of pancreatic cancer cell via E2F1-TK1-P21 axis. Cell Prolif 51:e12428. https://doi.org/10.1111/cpr.12428
Zuo S, Wang H, Li L, Pan H, Lu L (2022) Thymidine kinase 1 drives skin cutaneous melanoma malignant progression and metabolic reprogramming. Front Oncol 12:802807. https://doi.org/10.3389/fonc.2022.802807
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W. M. conceived the ideas. W. M. and Y. M. J. designed the experiments. W. M. performed the experiments. W. M. analyzed the data. W. M. provided the critical materials. W. M. wrote the manuscript. Y. M. J. supervised the study. All the authors have read and approved the final version for publication.
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Wu, M., Ye, M. Transcription factor Dp-1 knockdown downregulates thymidine kinase 1 expression to protect against proliferation and epithelial-mesenchymal transition in cervical cancer. Funct Integr Genomics 23, 301 (2023). https://doi.org/10.1007/s10142-023-01218-6
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DOI: https://doi.org/10.1007/s10142-023-01218-6