Abstract
Rohitukine is a chromone alkaloid and precursor of potent anticancer drugs flavopiridol, P-276–00, and 2,6-dichloro-styryl derivative (11d) (IIIM-290). The metabolite is reported to possess anticancer, anti-inflammatory, antiadipogenic, immunomodulatory, gastroprotective, anti-implantation, antidyslipidemic, anti-arthritic, and anti-fertility properties. However, the physiological role of rohitukine in plant system is yet to be explored. Here, we studied the effect of rohitukine isolated from Dysoxylum gotadhora on Arabidopsis thaliana. The A. thaliana plants grown on a medium fortified with different rohitukine concentrations showed a significant effect on the growth and development. The root growth of A. thaliana seedlings showed considerable inhibition when grown on medium containing 1.0 mM of rohitukine. Transcriptomic analysis indicated the expression of 895 and 932 genes in control and treated samples respectively at a cut-off of FPKM ≥ 1 and P-value < 0.05. Gene ontology (GO) analysis revealed the upregulation of genes related to photosynthesis, membrane transport, antioxidation, xenobiotic degradation, and some transcription factors (TFs) in response to rohitukine. Conversely, rohitukine downregulated several genes including RNA helicases and those involved in nitrogen compound metabolism. The RNA-seq result was also validated by real-time qRT-PCR analysis. In light of these results, we discuss (i) likely ecological importance of rohitukine in parent plant as well as (ii) comparison between responses to rohitukine treatment in plants and mammals.
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Data Availability
DGE values are available in the supplementary files. Raw transcriptome data may be made available on request.
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SGG is highly thankful to Council of Scientific and Industrial Research (India), grant numbers (MLP110006 and HCP38) for funding, SA and RC are thankful to Council of Scientific and Industrial Research-India for Senior Research Fellowship.
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S.A. conducted the experiments, carried out analysis, and wrote the entire manuscript; R.C. and V.L.J. helped in data generation and analysis; B.A.M. helped in figure preparation and manuscript writing, A.J. and J.T. helped in transcriptome raw data analysis; S.G.G. designed the study; supervised S.A.; R.C. and V.L.J. corrected the whole manuscript.
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Ahmed, S., Chouhan, R., Junaid, A. et al. Transcriptome analysis and differential expression in Arabidopsis thaliana in response to rohitukine (a chromone alkaloid) treatment. Funct Integr Genomics 23, 35 (2023). https://doi.org/10.1007/s10142-023-00961-0
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DOI: https://doi.org/10.1007/s10142-023-00961-0