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Promoter methylation regulates the abundance of osa-miR393a in contrasting rice genotypes under salinity stress

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Abstract

MicroRNAs (miRNAs) are important molecules that regulate gene expression under salinity stress. Despite their evolutionary conservation, these regulatory elements have been shown to behave differently in different plant species under a particular environmental stress. In this study, we investigated the behavior of salt responsive osa-miR393a and its target gene (TIR1, LOC_Os05g05800) in salt-tolerant (FL478) and salt-sensitive (IR29) rice genotypes. It was found that the mature and precursor sequences of osa-miR393a as well as its cleavage site in TIR1 were conserved among salt tolerant and sensitive genotypes. Promoters of different salt-responsive miRNAs were also found to be less variable between salt-tolerant and salt-susceptible genotypes. Analysis of gene expression, promoter methylation, and cis-element abundance showed that osa-miR393a behaves differently in FL478 and IR29. Salt stress altered the expression pattern of osa-miR393a-TIR1 module in a time-dependent manner in the roots and shoots of two genotypes. Promoter methylation of this regulatory module was also altered at different time points under salt stress. Expression analysis in two genotypes indicated the overall down-regulation of osa-miR393a and up-regulation of TIR1 in FL478 and their reciprocal regulation in IR29. The expression results were complemented by the differential promoter methylation and cis-element abundance of this regulatory module. Together, the results of transcript abundance and promoter methylation of osa-miR393a-TIR1 module signified the association between these two processes which is reported for the first time in plants to the best of our knowledge.

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Acknowledgments

The authors thank Dr. K.V. Bhat, Head, Division of Genomic Resources, NBPGR, New Delhi for his support and advice to carry out this work. Mr. Showkat Ahmad Ganie is grateful to the Department of Biotechnology, Government of India for the award of Senior Research Fellow.

Conflict of interest

The authors declare that there is no conflict of interest that could be perceived as prejudicing the impartiality of the reported research.

Funding

The authors are also grateful to Director, ICAR-NBPGR for funding the project through in-house grant (Project Code: IXX10476).

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Authors and Affiliations

  1. Division of Genomic Resources, National Bureau of Plant Genetic Resources, IARI Campus, New Delhi, 110012, India

    Showkat Ahmad Ganie & Tapan Kumar Mondal

  2. Department of Biotechnology, Visva-Bharati, Santiniketan, 731 235, West Bengal, India

    Narottam Dey

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  1. Showkat Ahmad Ganie
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Correspondence to Tapan Kumar Mondal.

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Ganie, S.A., Dey, N. & Mondal, T.K. Promoter methylation regulates the abundance of osa-miR393a in contrasting rice genotypes under salinity stress. Funct Integr Genomics 16, 1–11 (2016). https://doi.org/10.1007/s10142-015-0460-1

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