Abstract
We used transcriptome sequencing to investigate the hepatic postprandial responses of Rachycentron canadum (cobia), an important commercial fish species. In total, 150 cobia juveniles (50 per tank, triplicate) were fed ad libitum with a commercial diet for 7 days, fasted for 24 h, and fed for 10 min. The liver was sampled 10 min prior to feeding and 30 min, 1, 2, 4, 8, 12, and 24 h after the feeding event. Each sample was evaluated in terms of liver fatty acid profile and gene expression. Differential gene expressions were evaluated, focusing on fatty acid synthesis and oxidation pathways. In general, the liver fatty acid profile reflected diet composition. Docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), and n-3 long-chain polyunsaturated fatty acids (n-3 LC-PUFA) levels increased at 8 to 12 h but decreased at 24 h after the feeding event. A high number of differentially expressed genes (DEGs) were observed comparing fish that fasted for 8 h with those fasted for 30 min and 24 h, while a reduced number of DEGs was observed comparing individuals who fasted for 30 min compared with those who fasted for 24 h. Similarly, the main differences in the expression of genes related to the fatty acid biosynthesis and oxidation pathways were noticed in individuals who fasted for 8 h compared with those who fasted for 30 min and 24 h. The results suggested that the adequate time to sample the individuals ranged between 8 and 12 h after the meal since, apparently, after 24 h, differential gene expression was not necessarily influenced by food intake.
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Data Availability
Sequencing raw data were deposited in the Sequence Read Archive (SRA) repository of the National Center for Biotechnology Information (NCBI), under accession numbers SRR13009897, SRR13009896, SRR13009895, SRR13009894, SRR13009893, SRR13009892, SRR13009891, SRR13009890, SRR13009889, SRR13009888, SRR13009887, and SRR13009886, associated to the BioProject number PRJNA675281 and BioSamples numbers SAMN16708758, SAMN16708759, SAMN16708760, SAMN16708761, SAMN16708762, SAMN16708763, SAMN16708764, SAMN16708765, SAMN16708766, SAMN16708767, SAMN16708768, and SAMN16708769. The Transcriptome Shotgun Assembly (TSA) project has been deposited at DDBJ/EMBL/GenBank under accession number GIWT00000000. Intermediate data and high resolution figures can be found in the Open Science Framework (https://osf.io/ut5sn/).
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Funding
This study was supported by grants from the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP: 2019/26018–0). Bruno C. Araújo received a fellowship grant from São Paulo Research Foundation (FAPESP) (2016/12435–0). David Aciole Barbosa was a recipient of a scholarship grant from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES: 88882.365823/2019–01). A.W.S. Hilsdorf (309570/2021–2) and Renata G. Moreira (305493/2019–1) are recipients of CNPq productivity scholarships.
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Bruno C. Araújo, David Aciole Barbosa, Fabiano B. Menegidio, Giovana S. Branco, and Renato M. Honji: Conceptualization, data curation, formal analysis, investigation, writing, and editing. Victor H. Marques, Paulo H. de Mello, Marcelo V. Kitahara, and Artur N. Rombenso: Formal analysis, writing, review, and editing. Renata G. Moreira and Alexandre W.S. Hilsdorf: Conceptualization, methodology, resources, and review.
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Araújo, B.C., Barbosa, D.A., Honji, R.M. et al. Post-feeding Molecular Responses of Cobia (Rachycentron canadum): RNA-Sequencing as a Tool to Evaluate Postprandial Effects in Hepatic Lipid Metabolism. Mar Biotechnol 25, 358–371 (2023). https://doi.org/10.1007/s10126-023-10209-4
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DOI: https://doi.org/10.1007/s10126-023-10209-4