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Clonal relationship, virulence genes, and antimicrobial resistance of Morganella morganii isolated from community-acquired infections and hospitalized patients: a neglected opportunistic pathogen

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Abstract

Morganella morganii is a bacterium belonging to the normal intestinal microbiota and the environment; however, in immunocompromised individuals, this bacterium can become an opportunistic pathogen, causing a series of diseases, both in hospitals and in the community, being urinary tract infections more prevalent. Therefore, the objective of this study was to evaluate the prevalence, virulence profile, and resistance to antimicrobials and the clonal relationship of isolates of urinary tract infections (UTI) caused by M. morganii, both in the hospital environment and in the community of the municipality of Londrina-PR, in southern Brazil, in order to better understand the mechanisms for the establishment of the disease caused by this bacterium. Our study showed that M. morganii presents a variety of virulence factors in the studied isolates. Hospital strains showed a higher prevalence for the virulence genes zapA, iutA, and fimH, while community strains showed a higher prevalence for the ireA and iutA genes. Hospital isolates showed greater resistance compared to community isolates, as well as a higher prevalence of multidrug-resistant (MDR) and extended-spectrum beta lactamase (ESBL)-producing isolates. Several M. morganii isolates from both sources showed high genetic similarity. The most prevalent plasmid incompatibility groups detected were FIB and I1, regardless of the isolation source. Thus, M. morganii isolates can accumulate virulence factors and antimicrobial resistance, making them a neglected opportunistic pathogen.

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Acknowledgements

We thank the State University of Londrina and to the Laboratory of Virology of the State University of Londrina for the supply of the cell lines used in this study.

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This study was financed by the Coordination for the Improvement of Higher Education Personnel - Brazil (CAPES) Finance Code 001.

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Authors and Affiliations

  1. Laboratory of Bacteriology, Department of Microbiology, Center of Biological Sciences, State University of Londrina, Rodovia Celso Garcia Cid PO-BOX 6001, 86051-980, Londrina, Paraná, Brazil

    Luana Carvalho Silva, Matheus Silva Sanches, Gustavo Henrique Migliorini Guidone, Victor Hugo Montini, Bruno Henrique Dias de Oliva, Arthur Bossi do Nascimento & Sergio Paulo Dejato Rocha

  2. Laboratory of Virology, Department of Microbiology, Center of Biological Sciences, State University of Londrina, Londrina, Brazil

    Lígia Carla Faccin Galhardi

  3. Laboratory of Basic and Applied Bacteriology, Department of Microbiology, Center of Biological Sciences, State University of Londrina, Londrina, Brazil

    Renata Katsuko Takayama Kobayashi

  4. Department of Pathology, Clinical and Toxicological Analysis, Health Sciences Center, University Hospital of Londrina, State University of Londrina, Paraná, Brazil

    Eliana Carolina Vespero

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Contributions

All authors contributed to the study conception and design. Material preparation, data collection, and analysis were performed by Luana Carvalho Silva, Matheus Silva Sanches, Gustavo Henrique Migliorini Guidone, Victor Hugo Montini, Bruno Henrique Dias de Oliva, Arthur Bossi do Nascimento, and Lígia Carla Faccin Galhardi. The first draft of the manuscript was written by Renata Katsuko, Takayama Kobayashi, Eliana Carolina Vespero, and Sergio Paulo Dejato Rocha, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Sergio Paulo Dejato Rocha.

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Silva, L.C., Sanches, M.S., Guidone, G.H.M. et al. Clonal relationship, virulence genes, and antimicrobial resistance of Morganella morganii isolated from community-acquired infections and hospitalized patients: a neglected opportunistic pathogen. Int Microbiol 27, 411–422 (2024). https://doi.org/10.1007/s10123-023-00400-x

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