Abstract
Background
Emerging studies have shown that HOTAIR acts as an oncogene in gastric cancer (GC). However, its role in the extracellular matrix and in tumor immune infiltration remains unknown.
Methods
HOTAIR and COL5A1 levels were analyzed by bioinformatics analysis and validated by qRT-PCR, western blotting and immunohistochemistry assays. The regulatory relationships between components of the HOTAIR/miR-1277-5p/COL5A1 axis and the role of this axis in GC were predicted by bioinformatics analysis, and validated by in vitro and in vivo experiments. The correlation between COL5A1 and GC immune infiltration was assessed by bioinformatics analysis and a COL5A1-based predictive nomogram was established using the Stomach Adenocarcinoma dataset from The Cancer Genome Atlas.
Results
We found that HOTAIR and COL5A1 were overexpressed in GC compared to normal controls, which predicted poor prognosis. The regulatory relationship of the HOTAIR/miR-1277-5p/COL5A1 axis in GC was demonstrated, and HOTAIR and COL5A1 were found to promote GC growth while miR-1277-5p exerted the reverse effects. In addition, COL5A1 was negatively associated with tumor purity but positively associated with immune infiltration, which suggested that COL5A1-mediated GC growth may be partially mediated by the regulation of immune infiltration. Additionally, the established COL5A1-based nomogram showed that COL5A1 can serve as a prognostic biomarker in GC.
Conclusions
HOTAIR regulates GC growth by sponging miR-1277-5p and upregulating COL5A1, and COL5A1-mediated GC cell proliferation may be mediated by effects on the tumor microenvironment, which provides novel targets for GC treatment.
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Introduction
Approximately, 1,033,700 newly diagnosed gastric cancer (GC) cases and 782,700 GC-related deaths occurred worldwide in 2018, which makes it the 5th most common malignancy and the 3rd most common cause of cancer-related death in both sexes combined [1]. Although early detection, surgery, chemotherapy and molecular targeted therapy for GC have greatly progressed, the prognosis remains poor, with a 5-year survival rate of 30% for advanced GC and 75% for early GC due to inoperability and recurrence [2,3,4,5].
Long noncoding RNAs (lncRNAs), defined as those with lengths exceeding 200 bp, are involved in the initiation and development of tumors [6]. Homeobox transcript antisense intergenic RNA (HOTAIR), a trans-acting lncRNA with 2158 nucleotides and 6 exons, has been found to interact with polycomb repressive complex 2, leading to epigenetic silencing and tumorigenesis of many cancer types, including breast cancer and bladder cancer [7]. In GC, accumulating evidence has revealed that HOTAIR exerts its biological function by interacting with microRNAs (miRNAs) to regulate gene expression and participate in molecular pathways of tumorigenesis [8, 9]. Although the roles and underlying mechanisms of HOTAIR-mediated carcinogenesis in GC have been clarified, more novel functions of HOTAIR in GC need to be investigated.
The extracellular matrix (ECM), as a major component of the tumor environment, becomes disorganized in tumor formation and thus affects tumor development [10]. Collagen, a part of the ECM, is found to deposit greatly during tumorigenesis, and increased collagen deposition enhances tumor cell proliferation and immune cell infiltration [11,12,13]. Studies on collagen type V α1 chain (COL5A1) indicate that COL5A1 may be a new prognostic factor for patients with lung adenocarcinoma and that the miR-29b/COL5A1 axis could regulate bone metabolism and fibrogenic reactions [14, 15]. Bioinformatics analysis has revealed that COL5A1 is a prognostic indicator in GC prognosis [16], however, the role of COL5A1 in GC remains unknown. Hence, exploring the effects of COL5A1 on the occurrence and progression of GC and its underlying mechanisms is urgent.
In the present study, we found that HOTAIR and COL5A1 were overexpressed in GC cell lines and that overexpression of HOTAIR promoted the proliferation and metastasis of GC by sponging miR-1277-5p and upregulating COL5A1. In addition, overexpression of COL5A1-enhanced GC growth, possibly through facilitating immune infiltration, while miR-1277-5p mimics exerted the reverse effects on GC development. The established nomogram based on COL5A1 level indicated that COL5A1 was a prognostic factor for GC patients. Altogether, we concluded that the HOTAIR/miR-1277-5p axis regulates GC tumorigenesis by affecting COL5A1 levels, which may provide new targets for GC treatment.
Methods
RNA sequencing and microarray data analysis
GC gene expression data downloaded from Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) were analyzed to identify differentially expressed genes (DEGs) and lncRNAs (DElncRNAs). A heatmap of DElncRNAs was constructed with cut-off criteria of log2FC > 2.0 and adj. P value < 0.05, and volcano plots of DEGs were drawn with cut-off criteria of log2 |FC| > 1.0 and adj. P value < 0.05. Starbase [17], GEPIA [18], Kaplan–Meier analysis and log-rank test [19] were applied to predict the expression and overall survival (OS) of HOTAIR and DEGs in GC patients. Additionally, the binding sites between miR-1277-5p and its target genes were predicted by Starbase [17].
Pathway enrichment and biological function analysis
The protein–protein interaction (PPI) network of DEGs was obtained from the STRING database [20] and constructed by Cytoscape software (version 3.7.1). Molecular COmplex DEtection (MCODE) was used for cluster analysis, and Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were analyzed by ClueGO in Cytoscape software. Additionally, gene set enrichment analysis (GSEA) software (version 3.0) was used to identify pathways associated with COL5A1 in GC. The ESTIMATE algorithm was adopted to calculate the ESTIMATE, stromal and immune scores of GC tissues [21]. The Tumor IMmune Estimation Resource (TIMER) algorithm database was analyzed to explore the correlation between COL5A1 levels and 6 types of infiltrating immune cells [22].
Cell culture
All human GC and immortalized gastric cell lines purchased from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China) were cultured in Dulbecco's modified Eagle medium (DMEM, Gibco, USA) added with 10% fetal bovine serum (FBS, HyClone, USA), 100 KU/L penicillin and 100 mg/L streptomycin. Cells were maintained in an incubator at 37 °C with 5% CO2.
Transfection
GC cells were transfected with 0.4 μg of small interfering RNAs (siRNAs, against HOTAIR and COL5A1) or miR-1277-5p mimics or inhibitor (GenePharma, Shanghai, China) using Lipofectamine 2000 reagent (Invitrogen, USA). Overexpression of HOTAIR was achieved by transfecting 0.4 μg of pcDNA3.1 vector by cloning HOTAIR or COL5A1 sequence into GC cells, which were cultured for 48 h before the following experiments. The sequences of si-HOTAIRs, si-COL5A1s, miR-1277-5p mimics and inhibitor and their negative control (NC) are listed in Table S1.
Quantitative reverse transcription polymerase chain reaction (qRT-PCR)
TRIzol reagent (Invitrogen, USA) was applied to extract all total RNAs, and the cDNAs of HOTAIR and miR1277-5p were synthesized by a reverse transcriptase kit (M-MLV) (Takara, Dalian, China). QRT-PCR was conducted on the Bio-Rad real-time PCR system (Bio-Rad, USA) using the SYBR Premix Ex TaqTM kit (Roche, Switzerland). The relative HOTAIR, COL5A1 and miR1277-5p were calculated by the 2 –ΔΔCt formula, and GAPDH and U6 were regarded as the internal references. All primers are displayed in Table S2.
Western blotting
Equal amounts of protein samples (30 μg) were extracted by RIPA lysis buffer (Sigma, USA) and loaded onto 5% and 10% sodium dodecyl sulfate polyacrylamide gels for protein separation. After transferring protein to the polyvinylidene fluoride membrane (Millipore, USA) and blocking the membrane with 5% nonfat milk, the membrane was incubated with primary (1:1000, Abcam) and anti-rabbit secondary antibodies (1:5000, Thermo Fisher). The immune binding was detected using the ECL detection system (Peiqing Technology Co., Ltd, Shanghai, China). The optical density (OD) was normalized to that of β-actin (1:1000, Abcam) or GAPDH (1:2000, Abcam).
Immunohistochemistry
Human GC tissues were collected from patients who underwent gastrectomy in the First Affiliated Hospital of Anhui Medical University. The study was approved by the Ethics Committee of Anhui Medical University and written informed consent was obtained from these patients. After fixing with 4% paraformaldehyde, embedding in paraffin and slicing specimens into 5-μm thick sections, sections were deparaffinized and dehydrated. After antigen retrieval and endogenous peroxidase destruction, sections were incubated with anti-COL5A1 antibody overnight and then incubated with secondary antibody. Sections were counterstained with hematoxylin after treating horseradish peroxidase and 3,3′-diaminobenzidine. A light microscope was used to capture the images.
MTT and CCK-8 assay
A density of 10,000 cells per well were seeded in a 96-well plate to culture for 24 h. After transfection for 0, 24, 48, 72 h, 10 μL of MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium-bromide, 5 mg/mL, Ameresco, USA) was added to each well and cultured for 4 h, and then the solution above was discarded and the crystal was dissolved with DMSO (100 μL) for 15 min. Cell Counting Kit-8 (CCK-8; Beyotime, Shanghai, China) was also used to detect cell proliferation. After transfection for 48 h, 10 μL of CCK-8 reagent was added. The relative proliferation was calculated based on the absorbance of each well.
Ethynyldeoxyuridine (EdU) proliferation assay
After transfection, cell proliferation was assessed at 0, 24, 48 and 72 h using the BeyoClick™ EdU kit (Beyotime, Shanghai, China) according to the manufacturer’s instructions. EdU-positive cells were observed under fluorescence microscopy.
Transwell migration/invasion assay
Transwell chambers coated with Matrigel (BD Biosciences, USA) were used for the invasion assay and chambers without Matrigel were used for the migration assay. Briefly, after seeding 2 × 105 transfected cells into chambers (Corning Incorporated, USA), the upper wells were supplemented with DMEM without serum and the lower wells contained DMEM with 10% FBS; the cells were incubated for 72 h at 37 °C with 5% CO2. Then, the nonmigrating or noninvading cells were removed and the chambers were fixed with methanol and glacial acetic acid (3:1) for 30 min. Crystal violet was used to stain the cells for 15 min. An inverted microscope (Olympus, Tokyo, Japan) was applied to capture and count cells.
Wound healing assay
Transfected cells (5 × 105 cells) were seeded on the plates, and a 10-μL sterile tip was used to scratch the wound when the cells covered the plates. Pictures were obtained under the microscope at 0 and 24 h to calculate the width of the wound.
Dual-luciferase reporter assay
PmirGLO-HOTAIR/COL5A1-wt/mut (Gene Create, Wuhan, China) was constructed by cloning wild type (wt) or mutant (mut) HOTAIR/COL5A1 into the pmirGLO vector (Promega, USA). PmirGLO-HOTAIR/COL5A1-wt/mut (0.4 μg) was cotransfected with miR-1277-5p mimics or inhibitor (0.5 μL, 20 pmol/L) into 293 T cells using Lipofectamine 2000 (Invitrogen, USA). The Dual Luciferase Reporter Assay System (Promega, USA) was utilized to detect the luciferase activity and then normalized to the Renilla luciferase activity.
Xenograft tumor assay
Female nude mice (4 weeks old, 300–350 g) purchased from the Animal Center of Anhui Medical University were randomly allocated to two groups: the si-HOTAIR group and the si-NC group (n = 6 per group). A total of 100 μL of AGS cell suspension containing 2 × 106 cells transfected with si-HOTAIR or si-NC was subcutaneously injected into mice. Tumor size was measured from the fourteenth day after injection and then measured every 3 days. Measurement of mouse body weight was conducted every week. All mice were sacrificed at 1 month and tumors were completely detached from the mice for tumor weight measurement. H&E staining and immunohistochemistry were performed for morphological observation and COL5A1 expression detection, respectively. HOTAIR, miR-1277-5p and COL5A1 levels were determined by qRT-PCR.
Statistical analysis
GraphPad Prism 8.0 (San Diego, CA), Statistical package for social science (SPSS, version 22.0, IBM Corp., Armonk, NY, USA) and R software (version 3.6.1) were used to analyze all data that are expressed as the means ± standard deviation (SD). Two-tailed Student's t test was employed to evaluate the difference between two groups, and P < 0.05 was considered significant.
The Stomach Adenocarcinoma (STAD) dataset from TCGA was processed by deleting blank values for further analysis, and the thresholds used to divide patients by age, tumor grade, TNM stage, T stage, N stage, M stage, and percentage of positive lymph nodes were in accordance with other previous studies [23,24,25,26,27,28,29]. Based on the results of the χ2 test and LASSO regression, some important variables were selected, and Cox regression analysis was used to construct a nomogram using the rms package in R software. The performance of the model was assessed using the concordance index (C-index) and was internally validated using the bootstrap method with 1000 iterations, and the calibration curve, decision curve and clinical impact curve were plotted to visualize the agreement and net benefit of our model.
Results
HOTAIR is overexpressed in patients with GC and promotes the proliferation and metastasis of GC, which predicts a poor prognosis
A total of 55 abnormally expressed lncRNAs were identified in the TCGA-STAD dataset (Fig. 1a), and HOTAIR levels were significantly increased in GC samples compared to control samples (Fig. 1b). The results of the Starbase and GEPIA database analysis were in consistent with the results above (Fig. S1a, b), which was confirmed by qRT-PCR assay that HOTAIR was overexpressed in GC cell lines (Fig. 1c). Survival analysis indicated that patients with high levels of HOTAIR had shorter survival times (Fig. 1d). Then, we performed MTT, Transwell and wound healing assays to further test the role of HOTAIR in GC. First, the pcDNA-HOTAIR vector and three si-HOTAIRs were synthesized and transfected into AGS cells, and stably transfected cells were selected. As shown in Fig. 1e, the pcDNA-HOTAIR vector elevated HOTAIR levels, and three si-HOTAIRs knocked down HOTAIR expression effectively. MTT assay indicated that pcDNA-HOTAIR greatly promoted the proliferation of GC cells (Fig. 1f) and that si-HOTAIR-I/II exerted the reverse effects (Fig. 1g). The results of Transwell and wound healing assays revealed that cell migration, invasion and wound healing were increased in the pcDNA-HOTAIR group (Fig. 1h, i), in contrast, knockdown of HOTAIR presented the opposite effects (Fig. 1j, k).
HOTAIR exerts its biological roles by sponging miR-1277-5p and upregulating COL5A1
To elucidate the mechanisms underpinning HOTAIR-mediated cellular growth, we first plotted the volcano plots of four GSE datasets (GSE2685, GSE13911, GSE29272 and GSE54129) and a TCGA-STAD dataset, which illustrated DEGs in GC samples (Fig. 2a). After intersection analysis, 62 DEGs were identified (Fig. 2b), and a PPI network was constructed (Fig. 2c). Based on the MCODE cluster score, four clusters were established and we selected cluster 1 (score = 12.714) for further analysis (Fig. 2d). A total of 15 genes, including COL1A1, COL3A1, COL5A1, etc. were identified in cluster 1, and a correlation heatmap further revealed the relationship among these genes (Fig. S2a). GO and KEGG pathway analysis found that these genes were mainly enriched in extracellular matrix components, ECM-receptor interactions, etc. (Fig. 2e, f). Elevated expression of these 15 genes was negatively correlated with OS in GC patients (Fig. S3). Then, we identified COL5A1 as the downstream target gene of HOTAIR, and the qRT-PCR results indicated that the COL5A1 level was positively correlated with HOTAIR expression (Fig. 2g, r = 0.701, P = 0.0012). Previous studies elucidated that lncRNAs could function as ceRNAs by interacting with miRNAs to regulate tumorigenesis [8, 9, 30]. Hence, we speculated that HOTAIR may regulate COL5A1 by sponging miRNAs. The Venn diagram showed that HOTAIR and COL5A1 had 25 common miRNAs (Fig. S2b), which are listed in Table S3. As shown in Fig. S2c, binding sites between miR-1277-5p and HOTAIR or COL5A1 indicated that HOTAIR may regulate COL5A1 by sponging with miR-1277-5p. To confirm our hypothesis, qRT-PCR was conducted. The COL5A1 level was elevated in the pcDNA-HOTAIR group but decreased in the si-HOTAIR group (Fig. 2h), and miR-1277-5p exerted reverse effects on COL5A1 expression (Fig. 2i). The western blotting assay results were in line with the qRT-PCR assay results (Fig. 2j, k). Additionally, HOTAIR and miR-1277-5p negatively regulated each other’s expression (Fig. 2l, m).
Bioinformatics analysis predicted that direct binding sites existed between miR-1277-5p and HOTAIR or COL5A1, and a dual-luciferase reporter assay was performed to verify their interactions. HOTAIR/COL5A1-wt/mut sequences were synthesized, and pmirGLO-HOTAIR/COL5A1-wt/mut was constructed (Fig. S2c). Compared with that in the control group, the luciferase activity decreased significantly in the HOTAIR/COL5A1-wt and miR-1277-5p mimics cotransfection group of 293 T cells, while greatly increased luciferase activity was observed in the HOTAIR/COL5A1-wt and miR-1277-5p inhibitor cotransfection group (Fig. 2n, o). Conversely, transfection of HOTAIR/COL5A1-mut abolished the effects above.
Knockdown of HOTAIR restrains the growth of GC in vivo
The oncogenic effect ofHOTAIR was confirmed by subcutaneously injecting mice with AGS cells transfected with si-HOTAIR. As shown in Fig. 3a–d, tumor volume and weight increased more slowly in the si-HOTAIR group than in the control group. Images of H&E staining showed the morphology of tumor tissues (Fig. 3e). The immunohistochemistry results showed that the level of COL5A1 was significantly decreased in the si-HOTAIR group (Fig. 3f). COL5A1 and HOTAIR expression was decreased in the presence of si-HOTAIR, while miR-1277-5p levels were elevated when HOTAIR was knocked down (Fig. 3g). Moreover, linear regression analysis suggested that COL5A1 expression was positively correlated with the expression of HOTAIR (Fig. 3h, r = 0.92, P = 0.0088), while the expression of COL5A1 and HOTAIR was negatively correlated with the expression of miR-1277-5p (Fig. 3i, j, r = − 0.95, P = 0.0044; r = − 0.98, P = 0.0007).
MiR-1277-5p inhibits the growth and metastasis of GC cells.
To further explore the function of miR-1277-5p in GC development, in vitro experiments were conducted. Initially, the MTT assay revealed that miR-1277-5p mimics greatly suppressed the proliferation of AGS and HGC cells, while the miR-1277-5p inhibitor promoted proliferation (Fig. 4a, b). Additionally, the migration, invasion and wound healing abilities of GC cells were attenuated by upregulation of miR-1277-5p and enhanced by downregulation (Fig. 4c–h). Therefore, miR-1277-5p plays a vital role in the initiation and development of GC.
High expression of COL5A1 is associated with the development and immune infiltration of GC
The expression level of COL5A1 was increased in GC tissues according to analysis of the GEO and STAD datasets (Fig. 5a–e), the COL5A1 level was higher in the high stromal and immune score group than in the low score group (Fig. 5f, g), and was positively correlated with the ESTIMATE score (Fig. 5h, r = 0.54, P < 0.0001) as well as the stromal and immune score (Fig. S4a, b). As shown in Fig. 5i, the GSEA results showed that cell cycle, DNA replication and pathways in cancer, etc., were enriched in tissues with high COL5A1, which was validated by analysis of the TCGA-STAD dataset (Fig. S4c). In addition, we found that pathways of the immune-related pathway were also enriched (Fig. S4d), and analysis of the TIMER algorithm validated that COL5A1 expression was negatively correlated with tumor purity but positively correlated with CD8 + T cell, CD4 + T cell, macrophage, neutrophil and dendritic cell infiltration (Fig. S4e). To verify the findings above, we first found that COL5A1 was significantly elevated in GC tissues and cells (Fig. 5j–l). After confirming that si-COL5A1s effectively downregulated COL5A1 by western blotting and qRT-PCR assay (Fig. 5m, n), the CCK-8 assay was conducted, and the results showed that knockdown of COL5A1 greatly repressed the growth of AGS and HGC27 cells (Fig. 5o).
MiR-1277-5p rescues the oncogenic effects of HOTAIR on GC by regulating COL5A1
We cotransfected si-HOTAIR and miR-1277-5p inhibitor into GC cells and found that the miR-1277-5p inhibitor rescued the decrease in cell proliferation mediated by si-HOTAIR (Fig. 6a), and the results of the wound healing assay were consistent with those of the EdU assay (Fig. 6b, c). Simultaneously, the miR-1277-5p inhibitor was able to restore the effects of si-HOTAIR on HOTAIR and COL5A1 expression (Fig. 6d, e). In addition, miR-1277-5p mimics and pcDNA-COL5A1 were cotransfected into AGS and HGC27 cells, and the results showed that pcDNA-COL5A1 rescued the effects of miR-1277-5p mimics on COL5A1 expression (Fig. 6f). Taken together, we could conclude that HOTAIR upregulated COL5A1 by sponging miR-1277-5p, which disrupted the normal ECM to change the GC microenvironment, promoting immune infiltration, proliferation and metastasis of GC (Fig. 6g).
Prognostic nomogram for OS
To further explore the role of COL5A1 in OS, a COL5A1-based nomogram was formulated to predict the 1/3/5-year survival probability. As displayed in Table S4, tumor grade and T stage were significantly associated with COL5A1 expression. In addition, 8 variables [COL5A1, HOTAIR, gender, T stage, TNM stage, age, positive lymph nodes (positive LNs (%)] and residual tumor) were screened out by LASSO regression (Fig. 7a, b). Then, we used age, gender, tumor grade, positive LNs (%) and COL5A1 to build the prognostic nomogram (Fig. 7c). The C-index for OS prediction was 0.69 (95% CI 0.63–0.74), which was confirmed to be 0.67 by the bootstrap method. The calibration curves visualized the good agreement between the predicted OS and actually observed OS (Fig. 7d–f). Moreover, decision curve and clinical impact curve graphically suggested the clinical usefulness of the model based on a continuous threshold probability for OS prediction that the established model provided good net benefit (Fig. 7g, h).
Discussion
In our study, we explored the role of the HOTAIR/miR-1277-5p/COL5A1 axis in GC and its underlying mechanisms. Our major findings were as follows: (i) HOTAIR was highly expressed in GC cells and enhanced the proliferation and metastasis of GC, which was mediated by sponging miR-1277-5p and upregulating COL5A1; (ii) miR-1277-5p suppressed the growth and metastasis of GC, while COL5A1 promoted the growth of GC, and COL5A1 was positively correlated with GC immune infiltration; (iii) miR-1277-5p reversed the oncogenic effects of HOTAIR on GC by regulating COL5A1; and (iv) the COL5A1-based nomogram demonstrated that COL5A1 was a prognostic factor in patients with GC. Taken together, these findings elucidated that HOTAIR upregulated COL5A1 expression by sponging miR-1277-5p to promote the progression of GC, and intervening collagen (COL5A1) expression by lncRNAs may provide new insights into the treatment of GC.
As one of the most common malignancies and the 3rd leading cause of cancer-related death, GC imposes a heavy burden on patients and society [1]. Hence, exploring reliable biomarkers and therapeutic targets to improve the early diagnosis and prognosis for patients with GC is essential. Increasing evidence indicates that HOTAIR is engaged in various cancer processes, including epigenetic activation or silencing and ceRNA networks [31]. For example, the HOTAIR/miR-331-3p/HER2 and HOTAIR/miR-217 axes can enhance the growth, invasion and chemotherapy resistance of GC [8, 9]. In this study, we further demonstrated that HOTAIR promoted tumorigenesis and development of GC, which represented a poor prognosis in patients with GC. Interestingly, we found a novel miRNA (miR-1277-5p) that was engaged in HOTAIR-mediated GC progression, and HOTAIR regulated GC development by sponging miR-1277-5p.
Previous studies reported that the ECM is involved in tumorigenesis via processes including cell migration and proliferation [12, 32] and EMT [33]. Notably, collagen-containing fibers facilitated the rapid migration of tumor cells, and increased collagen density enhanced the proliferation and migration of tumor cells [12]. COL1 and COL11A1 have been proven to promote the malignant phenotype and progression of pancreatic ductal adenocarcinoma and head and neck squamous cell cancer [34,35,36]. Therefore, collagen exerts a regulatory role in the growth and invasion of tumors. Accumulating research indicates that COL5A1 may be a new prognostic factor for patients with lung adenocarcinoma [14]. In addition, a high level of COL5A1 accelerates the growth and progression of renal cell carcinoma and breast cancer [37, 38], which reminds us that ECM may play an important role in GC. In our study, we established an interaction network of ECM structural constituents, which were negatively related to the OS of GC patients. Additionally, bioinformatics analysis indicated that GC samples with high COL5A1 expression were positively associated with the cell cycle, DNA replication, etc. We further demonstrated that COL5A1 was highly expressed in GC cells and tissues, and knockdown of COL5A1 repressed the proliferation of GC cells, which was regulated by the HOTAIR/miR-1277-5p axis. HOTAIR-mediated cellular proliferation and metastasis was achieved by sponging miR-1277-5p and upregulating COL5A1.
Recently, increasing evidence has shown that tumor immune infiltration is a promising target of tumor treatment [39, 40], and collagen promotes the infiltration of immune cells in the tumor microenvironment to inhibit macrophages from killing cancer cells [10]. Coincidentally, our results suggested that COL5A1 was positively related to stromal, immune and ESTIMATE scores, implying that COL5A1 was negatively associated with tumor purity, an index representing the proportion of tumor cells in the tumor microenvironment. Specifically, we found that COL5A1 expression was negatively correlated with GC purity but positively correlated with CD8 + T cell, CD4 + T cell, macrophage, neutrophil and dendritic cell infiltration. Moreover, GC samples with high COL5A1 levels were positively associated with immune-related pathways including leukocyte transendothelial migration and natural killer cell-mediated cytotoxicity. Combined with previous studies showing that low tumor purity predicted poor prognosis in colon cancer, kidney renal clear cell carcinoma and lower grade glioma [41, 42], we were able to summarize that increased COL5A1 facilitated immune infiltration, which accelerated the proliferation and metastasis of tumors. Therefore, we speculated that COL5A1-mediated cell proliferation was partially achieved by an increase in immune cell infiltration, which may be regulated by the HOTAIR/miR-1277-5p axis. However, this needs to be further studied for verification.
To further validate the clinical usefulness of COL5A1, we formulated a COL5A1-based nomogram to predict the OS of GC patients, and this signature incorporated age, gender, tumor grade, positive LNs (%) and COL5A1 level. Further analyses showed the good agreement, performance, accuracy and net benefit of our model. Hence, COL5A1 may be a prognostic biomarker and new therapeutic target for GC treatment.
In conclusion, we demonstrated that COL5A1 played a vital role in the occurrence and progression of GC, which may be mediated by effects on the tumor microenvironment. In addition, COL5A1-mediated GC cell proliferation and metastasis were regulated by the HOTAIR/miR-1277-5p axis. Our findings imply that ECM could be a potential therapeutic target for GC treatment. However, further study is needed to explore the role of the ECM in tumor growth and immune infiltration, to determine whether the tumor microenvironment is regulated by lncRNA, and to clarify the therapeutic value of these components.
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Acknowledgements
The results here are partially based upon data generated by the GEO and TCGA Research Network.
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This work was supported by Natural Science Project of Anhui Province (No. KJ2017A829).
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ZJW and LC performed and analyzed most of the experiments. LM provided intellectual and technical support for the in vitro and in vivo experiments. ZJW, LC, WXH, and AMX oversaw, designed and revised the study and experiments, analyzed the data, and cowrote the manuscript. LH made significant, marked, and substantial contributions to the revision of this work.
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Wei, Z., Chen, L., Meng, L. et al. LncRNA HOTAIR promotes the growth and metastasis of gastric cancer by sponging miR-1277-5p and upregulating COL5A1. Gastric Cancer 23, 1018–1032 (2020). https://doi.org/10.1007/s10120-020-01091-3
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DOI: https://doi.org/10.1007/s10120-020-01091-3