Abstract
The conformation of silk fibroin (SF) frozen with polyethylene glycol (PEG) at a molecular weight from 2 kDa to 20 kDa and a mass ratio of PEG:SF from 1:5 to 10:1 was studied by spectral and microscopic methods. It is found that the conformation transition of SF from random coil to β-sheet could be induced by the stress resulting from PEG crystallization at −20 °C, and greatly depended on the cooling rate, PEG:SF mass ratio and PEG molecular weight. These findings provide a new method for the preparation of desired SF nanofibers.
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This work was financially supported by the National Natural Science Foundation of China (No. 21374022).
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Chen, Ch., Zhao, J., Yang, Z. et al. Nanofibers of silk fibroin controlled by the crystallization of polyethylene glycol in frozen solution. Chin J Polym Sci 35, 1373–1380 (2017). https://doi.org/10.1007/s10118-017-1974-x
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DOI: https://doi.org/10.1007/s10118-017-1974-x