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Association of Papacarie Duo® and low-level laser in antimicrobial photodynamic therapy (aPDT)

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Abstract

Dental caries is a multifactorial, non-communicable disease. Effective treatment options for minimally invasive removal of carious tissue include Papacarie Duo® gel and antimicrobial photodynamic therapy (aPDT). aPDT involves a combination of a light source and photosensitizer. Given that Papacarie Duo® contains a percentage of blue dye, this study aims to explore the antimicrobial potential of Papacarie Duo® when associated with a light source against Streptococcus mutans strains. The chosen light source was a low-power diode laser (λ = 660 nm, E = 3 J, P = 100 mW, t = 30 s). To assess antimicrobial capacity, planktonic suspensions of Streptococcus mutans were plated on Brain Heart Infusion Agar (BHI) to observe the formation of inhibition halos. The studied groups included methylene blue (0.005%), Papacarie Duo®, distilled water (negative control), 2% chlorhexidine (positive control), Papacarie Duo® + laser, and methylene blue (0.005%) + laser. Following distribution onto plates, each group was incubated at 37 °C for 48 h under microaerophilic conditions. Inhibition halos were subsequently measured using a digital caliper. The results showed that chlorhexidine had the greatest antimicrobial effect followed by the group of irradiated methylene blue and irradiated Papacarie Duo®. All experimental groups demonstrated antimicrobial potential, excluding the negative control group. The study concludes that Papacarie Duo® exhibits antimicrobial properties when associated with a low-power diode laser.

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Funding

This study was funded by the Foundation for Support to Research and Scientific and Technological Development of Maranhão (FAPEMA), process no. 04981/2018.

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Authors and Affiliations

  1. Department of Dentistry I, Federal University of Maranhão (UFMA), Avenida Dos Portugueses, 1966, Vila Bacanga, São Luís, MA, 65080-805, Brazil

    Roberta Janaina Soares Mendes, Nayanna Matos de Sousa & Guilherme Silva Furtado

  2. Federal University of Minas Gerais (UFMG), Belo Horizonte, Brazil

    Marco Aurélio Benini Paschoal

  3. Federal University of Maranhão (UFMA), São Luís, Brazil

    Andréa Dias Neves Lago

Authors
  1. Roberta Janaina Soares Mendes
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  2. Nayanna Matos de Sousa
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  4. Marco Aurélio Benini Paschoal
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  5. Andréa Dias Neves Lago
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Contributions

RJSM: formal analysis, writing—original draft, writing—review and editing, visualization. NMS: conceptualization, methodology, investigation, resources, writing—original draft, writing—review and editing, visualization, funding acquisition. GSF: validation. MABP: supervision, formal analysis. ADNL: resources, writing—original draft, writing—review and editing, project administration, funding acquisition.

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Correspondence to Roberta Janaina Soares Mendes.

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Highlights

1. The chemical-mechanical technique for partial removal of carious tissue is more effective and better than the manual one in terms of dentin preservation.

2. Photodynamic therapy presents itself as an extra technique for antibacterial action in caries lesions.

3. Papacarie Duo® alone and associated with low-power laser showed that the statistical differences were minimal.

4. Photodynamic therapy is a non-invasive and efficient alternative for the control of antimicrobial activity.

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Mendes, R.J.S., de Sousa, N.M., Furtado, G.S. et al. Association of Papacarie Duo® and low-level laser in antimicrobial photodynamic therapy (aPDT). Lasers Med Sci 39, 25 (2024). https://doi.org/10.1007/s10103-024-03981-9

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