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High-frequency near-infrared semiconductor laser irradiation suppressed experimental tooth movement-induced inflammatory pain markers in the periodontal ligament tissues of rats

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Abstract

High-frequency near-infrared (NIR) semiconductor laser-irradiation has an unclear effect on nociception in the compressed lateral periodontal ligament region, a peripheral nerve region. This study aimed to investigate the effects of NIR semiconductor laser irradiation, with a power of 120 J, on inflammatory pain markers and neuropeptides induced in the compressed lateral periodontal ligament area during ETM. A NIR semiconductor laser [910 nm wavelength, 45 W maximum output power, 300 mW average output power, 30 kHz frequency, and 200 ns pulse width (Lumix 2; Fisioline, Verduno, Italy)] was used. A nickel-titanium closed coil that generated a 50-g force was applied to the maxillary left-side first molars and incisors in 7-week-old Sprague–Dawley (280–300 g) rats to induce experimental tooth movement (ETM) for 24 h. Ten rats were divided into two groups (ETM + laser, n = 5; ETM, n = 5). The right side of the ETM group (i.e., the side without induced ETM) was evaluated as the untreated group. We performed immunofluorescent histochemistry analysis to quantify the interleukin (IL)-1β, cyclooxygenase-2 (COX2), prostaglandin E2 (PGE2), and neuropeptide [calcitonin gene-related peptide (CGRP)] expression in the compressed region of the periodontal tissue. Post-hoc Tukey–Kramer tests were used to compare the groups. Compared with the ETM group, the ETM + laser group showed significant suppression in IL-1β (176.2 ± 12.3 vs. 310.8 ± 29.5; P < 0.01), PGE2 (104.4 ± 14.34 vs. 329.6 ± 36.52; P < 0.01), and CGRP (36.8 ± 4.88 vs. 78.0 ± 7.13; P < 0.01) expression. High-frequency NIR semiconductor laser irradiation exerts significant effects on ETM-induced inflammation. High-frequency NIR semiconductor laser irradiation can reduce periodontal inflammation during orthodontic tooth movement.

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Acknowledgements

The authors would like to thank Emeritus Professor Kazuo Tanne of the Department of Orthodontics and Craniofacial Developmental Biology, Hiroshima University Graduate School of Biomedical Sciences, for kindly providing advice to this work. This work was performed at the Research Center for Molecular Medicine, Hiroshima University.

Funding

This work was funded by grants from the Japan Society for Promotions of Science (grant numbers: 19K10385, 20K10226 and 21K21041).

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Authors and Affiliations

  1. Department of Orthodontics and Craniofacial Developmental Biology, Hiroshima University Graduate School of Biomedical Sciences, 1-2-3 Kasumi, Minami-ku, Hiroshima, 734-8553, Japan

    Ayaka Nakatani, Ryo Kunimatsu, Yuji Tsuka, Shuzo Sakata, Shota Ito, Isamu Kado, Nurul Aisyah Rizky Putranti & Kotaro Tanimoto

  2. Department of Maxillofacial Anatomy and Neuroscience, Graduate School of Biomedical & Health Sciences, Hiroshima University, Hiroshima, Japan

    Ryuji Terayama

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  1. Ayaka Nakatani
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Contributions

Ayaka Nakatani conducted the main experiments and prepared the manuscript. Ayaka Nakatani and Ryo Kunimatsu analyzed the data and contributed clarifications and guidance on the manuscript. Ryo Kunimatsu and Yuji Tsuka conceived and designed the experiments. Ayaka Nakatani, Shuzou Sakata, and Shota Ito performed the experiments. Ayaka Nakatani,Ryo Kunimatsu, Isamu Kado, Shota Ito and Nurul Aisyah Rizky Putranti prepared the manuscript. Totaro Tanimoto and Ryuji Terayama designed and supervised the study.

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Correspondence to Ryo Kunimatsu.

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All experimental protocols were approved by the Ethics Committee for Animal Experiments of Hiroshima University School of Dentistry (approval number A17-187). All procedures performed in the study were in accordance with the ethical standards of the institutional research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.

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Nakatani, A., Kunimatsu, R., Tsuka, Y. et al. High-frequency near-infrared semiconductor laser irradiation suppressed experimental tooth movement-induced inflammatory pain markers in the periodontal ligament tissues of rats. Lasers Med Sci 38, 109 (2023). https://doi.org/10.1007/s10103-023-03761-x

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