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Photobiomodulation alters the viability of HUVECs cells

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Abstract

The aim of the present study was to investigate the influence of low-level red (660 nm) and infrared (780 nm) laser with four different radiance exposures on human umbilical vein endothelial cells (HUVECs) in vitro. HUVECs (1.5 × 104) were incubated in 96-well culture plates. The cells were maintained in M199 medium supplemented with 20% fetal bovine serum, 1% antibiotic (penicillin), 1% anti-mycotic (Fungizone), and 1% endothelial cell growth supplement. After centrifugation, irradiations (660/780 nm, 40 mW, 1, 5, 10, and 20 J/cm2, 1 s, 5 s, 10 s, and 20 s, respectively, total energy 0.4 J, 2 J, 4 J, and 8 J, and beam spot size at target 0.04 cm2) were performed at the bottom of Falcon tubes such that the laser beam directly reached the cell without passing through the culture medium. The cells were divided into groups based on radiant exposures. Cell viability and protein concentration were verified after 1, 2, 3, 6, 8, and 10 days. Red laser increased the cell viability and protein concentration in all groups (three-way ANOVA, p < 0.05) beginning on the second day. The greatest peak compared with the control was found when the radiant exposure was 5 J/cm2 and 10 J/cm2. Infrared laser inhibited cell viability and modulated the protein concentration in the cells, with the highest peak protein concentration found on the second day in the group with radiant exposure of 1 J/cm2 and 10 J/cm2 (three-way ANOVA, p < 0.05). Red laser increased the viability and concentration of total proteins in HUVECs, whereas infrared laser had an inhibitory effect on cell viability, while maintaining the total protein concentration similar to that found in the control group.

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Funding

This work was supported by UNINOVE and the Brazilian fostering agency Coordination for the Advancement of Higher Education Personnel- CAPES (www.capes.gov.br; process number 014704/2013-07 SMLT).

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Authors and Affiliations

  1. Biophotonics Applied to Health Sciences, Universidade Nove de Julho – UNINOVE, São Paulo, SP, Brazil

    Stella Maris Lins Terena, Raquel Agnelli Mesquita-Ferrari & Kristianne Porta Santos Fernandes

  2. Rehabilitation Sciences, Universidade Nove de Julho –UNINOVE, São Paulo, SP, Brazil

    Raquel Agnelli Mesquita-Ferrari & Andreia Martinelli de Siqueira Araújo

  3. Faculty of Dental Medicine, U. Porto - FMDUP, Porto, Portugal

    Maria Helena Fernandes

  4. LAVQ/REQUIMTE, U. Porto, Porto, Portugal

    Maria Helena Fernandes

Authors
  1. Stella Maris Lins Terena
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  2. Raquel Agnelli Mesquita-Ferrari
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  3. Andreia Martinelli de Siqueira Araújo
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  4. Kristianne Porta Santos Fernandes
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  5. Maria Helena Fernandes
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Correspondence to Raquel Agnelli Mesquita-Ferrari.

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The authors declare that they have no competing interests.

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This study received approval from Ethics Committee on Animal Research of University Nove de Julho (process number AN007/2013).

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Terena, S.M.L., Mesquita-Ferrari, R.A., de Siqueira Araújo, A.M. et al. Photobiomodulation alters the viability of HUVECs cells. Lasers Med Sci 36, 83–90 (2021). https://doi.org/10.1007/s10103-020-03016-z

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  • DOI: https://doi.org/10.1007/s10103-020-03016-z

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