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Thermal coagulum formation and hemostasis during repeated multipulse Nd:YAG laser treatment of cutaneous vascular lesions: animal experiment study

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Abstract

Laser therapy has been widely used to treat port-wine stain (PWS) and other cutaneous vascular lesions via selective photothermolysis. High incident laser fluence is always prohibited in clinic to prevent the thermal damage in normal skin tissue, leading to insufficient energy deposition on the target blood vessel and incomplete clearance of PWS lesion. In this study, repeated multipulse laser (RMPL) irradiation was proposed to induce acute thermal damage to target blood vessels with low incident fluence (40 J/cm2 for 1064-nm Nd:YAG laser). The feasibility of the method was investigated using animal models. Repeated multipulse irradiation cycles with 10-min intervals were performed in RMPL. A hamster dorsal skin chamber model with a visualization system was constructed to investigate the instant generation of thermal coagulum and relevant hemostasis by thrombus formation during and after irradiation under 1064 nm Nd:YAG single multipulse laser (SMPL) and RMPL irradiation. The diameter of the target blood vessel and the size of thermal coagula were measured before and after laser irradiation. The reflectance spectra of the dorsal skin were measured by a reflectance spectrometer during RMPL. Stasis thermal coagula that clogged the vessel lumen were generated during SMPL irradiation with low incident fluence. However, there was no acute thermal damage of blood vessels. Reflectance spectra measurement showed that the generation of thermal coagula and subsequent thrombus formation increases blood absorption by more than 10% within the first 10 min after laser irradiation. Acute vessel thermal damage could be induced in the target blood vessel by RMPL with low incident fluence of 40 J/cm2. Compared with our previous SMPL study, nearly 30% reduction in incident laser fluence was achieved by RMPL. Low fluence RMPL may be a promising approach to improve the therapeutic outcome for patients with cutaneous vascular lesions by improving energy deposition on the target blood vessel.

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Funding

This study was funded by the National Natural Science Foundation of China (Grant No. 51727811) and China Postdoctoral Science Foundation (Grant No. 2019M653616).

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Authors and Affiliations

  1. State Key Laboratory of Multiphase Flow in Power Engineering, Xi’an Jiaotong University, Xi’an, 710049, China

    Dong Li, WenJuan Wu, Bin Chen, Zheming Gou, Penghui Zhao & GuoXiang Wang

  2. Department of Mechanical Engineering, University of Akron, Akron, OH, 44325, USA

    GuoXiang Wang

Authors
  1. Dong Li
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  2. WenJuan Wu
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  3. Bin Chen
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  4. Zheming Gou
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  5. Penghui Zhao
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  6. GuoXiang Wang
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Correspondence to Bin Chen.

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The authors declare that they have no conflict of interest.

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All procedures involving animal experiments were approved by the Institutional Animal Care and Use Committees of the Xi’an Jiaotong University.

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Li, D., Wu, W., Chen, B. et al. Thermal coagulum formation and hemostasis during repeated multipulse Nd:YAG laser treatment of cutaneous vascular lesions: animal experiment study. Lasers Med Sci 35, 1589–1597 (2020). https://doi.org/10.1007/s10103-020-03007-0

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  • DOI: https://doi.org/10.1007/s10103-020-03007-0

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