Skip to main content

Advertisement

SpringerLink
Log in

Irradiation with blue light-emitting diode enhances osteogenic differentiation of stem cells from the apical papilla

  • Original Article
  • Published:
Lasers in Medical Science Aims and scope Submit manuscript

Abstract

This study aimed to evaluate the effects of low-energy blue LED irradiation on the osteogenic differentiation of stem cells from the apical papilla (SCAPs). SCAPs were derived from human tooth root tips and were irradiated with 0 (control group), 1 J/cm2, 2 J/cm2, 3 J/cm2, or 4 J/cm2 blue light in osteogenic induction medium. Cell proliferation was analyzed using the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay. Osteogenic differentiation activity was evaluated by monitoring alkaline phosphatase (ALP), alizarin red staining, and real-time polymerase chain reaction (RT-PCR). The results of the MTT assay indicated that SCAPs in the LED groups exhibited a lower proliferation rate than those in the control group, and there were statistically differences between the 2 J/cm2, 3 J/cm2, and 4 J/cm2 groups and the control group (P < 0.05). The results of the ALP and alizarin red analyses showed that blue LED promoted osteogenic differentiation of the SCAPs. And 4 J/cm2 blue light upregulates the expression levels of the osteogenic/dentinogenic genes ALP, dentin sialophosphoprotein (DSPP), dentin matrix protein-1 (DMP-1), and osteocalcin (OCN) in SCAPs. Our results confirmed that low-energy blue LED at 1 J/cm2, 2 J/cm2, 3 J/cm2, and 4 J/cm2 could inhibit the proliferation of SCAPs and promotes osteogenic differentiation of SCAPs. Further in vitro studies are required to explore the mechanisms of the effects by low-energy blue LED.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. Dixin C, Hongyu L, Mian W et al (2018) The origin and identification of mesenchymal stem cells in teeth: from odontogenic to non-odontogenic. Current Stem Cell Research & Therapy 13(1):39–45

    Google Scholar 

  2. Sonoyama W, Liu Y, Fang D et al (2006) Mesenchymal stem cell-mediated functional tooth regeneration in swine. PLoS One 1:e79

    Google Scholar 

  3. Nada OA, El BRM (2018) Stem cells from the apical papilla (SCAP) as a tool for endogenous tissue regeneration. Frontiers in Bioengineering and Biotechnology 6:103

    Google Scholar 

  4. Huang TJ, Sonoyama W, Liu Y et al (2008) The hidden treasure in apical papilla: the potential role in pulp/dentin regeneration and bioroot engineering. J Endod 34(6):645–651

    Google Scholar 

  5. Shiehzadeh V, Aghmasheh F, Shiehzadeh F et al (2014) Healing of large periapical lesions following delivery of dental stem cells with an injectable scaffold: new method and three case reports. Indian J Dent Res 25(2):248

    Google Scholar 

  6. Bakopoulou A, Leyhausen G, Volk J et al (2011) Comparative analysis of in vitro osteo/odontogenic differentiation potential of human dental pulp stem cells (DPSCs) and stem cells from the apical papilla (SCAP). Arch Oral Biol 56(7):709–721

    CAS  Google Scholar 

  7. Pereira MC, de Pinho CB, Medrado AR et al (2010) Influence of 670 nm low-level laser therapy on mast cells and vascular response of cutaneous injuries. J Photochem Photobiol B Biol 98(3):188–192

    CAS  Google Scholar 

  8. Basso FG, Oliveira CF, Kurachi C et al (2013) Biostimulatory effect of low-level laser therapy on keratinocytes in vitro. Lasers Med Sci 28(2):367–374

    Google Scholar 

  9. Pasternak-Mnich K, Ziemba B, Szwed A et al (2019) Effect of photobiomodulation therapy on the increase of viability and proliferation of human mesenchymal stem cells. Lasers Surg Med

  10. Kim JE, Woo YJ, Sohn KM et al (2017) Wnt/β-catenin and ERK pathway activation: a possible mechanism of photobiomodulation therapy with light-emitting diodes that regulate the proliferation of human outer root sheath cells. Lasers Surg 49(10):940–947

    Google Scholar 

  11. Oliveira FA, Matos AA, Santesso MR et al (2016) Low intensity lasers differently induce primary human osteoblast proliferation and differentiation. J Photochem Photobiol B 163:14–21

    CAS  Google Scholar 

  12. Konig CJ, Buhner M, Murling G (2010) Blue-light irradiation regulates proliferation and differentiation in human skin cells. J Investig Dermatol 130(1):259

    Google Scholar 

  13. Arnolda G, Chien TD, Hayen A et al (2018) A comparison of the effectiveness of three LED phototherapy machines, single- and double-sided, for treating neonatal jaundice in a low resource setting. PLoS One 13(10):e0205432

    Google Scholar 

  14. Higuchi A, Shen PY, Zhao JK et al (2011) Osteoblast differentiation of amniotic fluid-derived stem cells irradiated with visible light. Tissue Eng A 17(21–22):2593–2602

    CAS  Google Scholar 

  15. Ginani F, Soares DM, Alexandre DORH et al (2018) Low-level laser irradiation induces in vitro proliferation of stem cells from human exfoliated deciduous teeth. Lasers Med Sci 33(1):95–102

    Google Scholar 

  16. Diao S, Lin X, Wang L et al (2017) Analysis of gene expression profiles between apical papilla tissues, stem cells from apical papilla and cell sheet to identify the key modulators in MSCs niche. Cell Prolif 50(3):e12337

    Google Scholar 

  17. Pagin MT, de Oliveira FA, Oliveira RC et al (2014) Laser and light-emitting diode effects on pre- osteoblast growth and differentiation. Lasers Med Sci 29(1):55–59

    Google Scholar 

  18. Zhu T, Wu Y, Zhou X et al (2019) Irradiation by blue light-emitting diode enhances osteogenic differentiation in gingival mesenchymal stem cells in vitro. Lasers Med Sci 34(7):1473–1481

    Google Scholar 

  19. Huang TJ, Gronthos S, Shi S (2009) Mesenchymal stem cells derived from dental tissues vs. those from other sources: their biology and role in regenerative medicine. J Dent Res 88(9):792–806

    CAS  Google Scholar 

  20. Chueh LH, Huang TJ (2006) Immature teeth with periradicular periodontitis or abscess undergoing apexogenesis: a paradigm shift. J Endod 32(12):1205–1213

    Google Scholar 

  21. Estrela C, Alencar AH, Kitten GT et al (2011) Mesenchymal stem cells in the dental tissues: perspectives for tissue regeneration. Braz Dent J 22(2):91–98

    Google Scholar 

  22. Diogenes A, Hargreaves KM (2017) Microbial modulation of stem cells and future directions in regenerative endodontics. J Endod 43(9):S95–S101

    Google Scholar 

  23. Chrepa V, Pitcher B, Henry MA et al (2017) Survival of the apical papilla and its resident stem cells in a case of advanced pulpal necrosis and apical periodontitis. J Endod 43(4):561–567

    Google Scholar 

  24. Lin LM, Kim SG, Martin G et al (2018) Continued root maturation despite persistent apical periodontitis of immature permanent teeth after failed regenerative endodontic therapy. Australian Endodontic Journal the Journal of the Australian Society of Endodontology Inc 44(3):292–299

    Google Scholar 

  25. Huang GTJ, Yamaza T, Shea LD et al (2010) Stem/progenitor cell–mediated de novo regeneration of dental pulp with newly deposited continuous layer of dentin in an in vivo model. Tissue Eng A 16(2):605–615

    CAS  Google Scholar 

  26. Na S, Zhang H, Huang F et al (2013) Regeneration of dental pulp/dentine complex with a three-dimensional and scaffold-free stem-cell sheet-derived pellet. J Tissue Eng Regen Med 10(3):261–270

    Google Scholar 

  27. Yan M, Wang L, Lei G et al (2013) Proliferation and osteo/odontoblastic differentiation of stem cells from dental apical papilla in mineralization-inducing medium containing additional KH2PO4. Cell Prolif 46(2):214–222

    Google Scholar 

  28. Marques, Márcia Martins, Diniz, Ivan a Márcia Alves et al (2016) Photobiomodulation of dental derived mesenchymal stem cells: a systematic review. Photomed Laser Surg 34(11):500–508

  29. Lipovsky A, Nitzan Y, Gedanken A, Lubart R (2010) Visible light-induced killing of bacteria as a function of wavelength: implication for wound healing. Lasers Surg Med 42(6):467–472

    Google Scholar 

  30. Alghamdi KM, Kumar A, Moussa NA (2012) Low-level laser therapy: a useful technique for enhancing the proliferation of various cultured cells. Lasers Med Sci 27(1):237–249

    Google Scholar 

  31. Barboza CAG, Ginani F, Soares DM et al (2014) Low-level laser irradiation induces in vitro proliferation of mesenchymal stem cells. Einstein (Sao Paulo) 12(1):75–81

    Google Scholar 

  32. Soares D M , Ginani F , Henriques, ág uida Gomes et al (2015) Effects of laser therapy on the proliferation of human periodontal ligament stem cells. Lasers Med Sci 30(3):1171–1174

  33. Mvula B, Mathope T, Moore T et al (2008) The effect of low level laser irradiation on adult human adipose derived stem cells. Lasers Med Sci 23(3):277–282

    CAS  Google Scholar 

  34. Zaccara IM, Ginani F, Mota-Filho HG et al (2015) Effect of low-level laser irradiation on proliferation and viability of human dental stem cells. Lasers Med Sci 30(9):2259–2264

    Google Scholar 

  35. Whelan HT, Smits RL, Buchmann EV et al (2001) Effect of NASA light-emitting diode irradiation on wound healing. J Clin Laser Med Surg 19(6):305–314

    CAS  Google Scholar 

  36. Posten W, Wrone DA, Dover JS et al (2005) Low-level laser therapy for wound healing: mechanism and efficacy. Dermatol Surg 31(3):334–340

    CAS  Google Scholar 

  37. Owen TA, Aronow M, Shalhoub V et al (1990) Progressive development of the rat osteoblast phenotype in vitro: reciprocal relationships in expression of genes associated with osteoblast proliferation and differentiation during formation of the bone extracellular matrix. J Cell Physiol 143(3):420–430

  38. Turrioni APS, Basso FG, Montoro LA et al (2014) Phototherapy up-regulates dentin matrix proteins expression and synthesis by stem cells from human-exfoliated deciduous teeth. J Dent 42(10):1292–1299

    CAS  Google Scholar 

  39. Li WT, Leu YC (2007) Effects of low level red-light irradiation on the proliferation of mesenchymal stem cells derived from rat bone marrow. Conf Proc IEEE Eng Med Biol Soc 2007:5830–5833

    Google Scholar 

  40. Soleimani M, Abbasnia E, Fathi M et al (2012) The effects of low-level laser irradiation on differentiation and proliferation of human bone marrow mesenchymal stem cells into neurons and osteoblasts—an in vitro study. Lasers Med Sci 27(2):423–430

    Google Scholar 

  41. Ballini A, Mastrangelo F, Gastaldi G et al (2015) Osteogenic differentiation and gene expression of dental pulp stem cells under low-level laser irradiation: a good promise for tissue engineering. J Biol Regul Homeost Agents 29(4):813–822

    CAS  Google Scholar 

  42. Borzabadi-Farahani A (2016) Effect of low-level laser irradiation on proliferation of human dental mesenchymal stem cells; a systemic review. J Photochem Photobiol B Biol 162:577–582

    CAS  Google Scholar 

  43. Li R, Peng L, Ren L et al (2009) Hepatocyte growth factor exerts promoting functions on murine dental papilla cells. J Endod 35(3):382–388

    Google Scholar 

  44. Feng JQ, Huang H, Lu Y et al (2003) The dentin matrix protein 1 (Dmp1) is specifically expressed in mineralized, but not soft, tissues during development. J Dent Res 82(10):776–780

    CAS  Google Scholar 

  45. Wang S, Mu J, Fan Z et al (2012) Insulin-like growth factor 1 can promote the osteogenic differentiation and osteogenesis of stem cells from apical papilla. Stem Cell Res 8(3):346–356

    CAS  Google Scholar 

Download references

Funding

This work was supported by the Luzhou Municipal People’s Government-Southwest Medical University science and technology strategic cooperation projects of China (no. 2017LZXNYD-T03), Luzhou Municipal Science and Technology Bureau of China (no. 2016-R-70(13/24)). The reagents of this study were supported by these funds that all came from Southwest Medical University.

Author information

Author notes

  1. Yaoyao Yang and Tingting Zhu contributed equally to this work.

Authors and Affiliations

  1. Southwest Medical University, Luzhou, 646000, China

    Yaoyao Yang, Chunxia Shu, Qiang Chen, Juan Yang & Xiang Luo

  2. Hospital/School of Stomatology, Zunyi Medical University, Zunyi, 563000, China

    Yaoyao Yang

  3. Yantai Stomatological Hospital, Yantai, 264000, China

    Tingting Zhu

  4. West China-Guang’an Hospital, Sichuan University, Guang’an, 638550, China

    Yan Wu

  5. Hospital of Stomatology Southwest Medical University, Luzhou, 646000, China

    Yao Wang

Authors
  1. Yaoyao Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Tingting Zhu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yan Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Chunxia Shu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Qiang Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Juan Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Xiang Luo
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Yao Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

Yaoyao Yang and Tingting Zhu designed the research, conducted the experiments, and wrote the paper. Yan Wu and Chunxia Shu conducted the experiments. Qiang Chen, Juan Yang, and Xiang Luo performed the data analyses and edited the manuscript. Yao Wang designed the research, supervised the study, and wrote the manuscript.

Corresponding author

Correspondence to Yao Wang.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All procedures performed in the study were in accordance with the Ethics Committee of the Affiliated Hospital of Stomatology Southwest Medical University Certificate (contract grant 20180314001) and with the 1964 Declaration of Helsinki and its later amendments or comparable ethical standards.

Informed consent

Informed consent was obtained from all individual participants included in the study.

Additional information

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Yang, Y., Zhu, T., Wu, Y. et al. Irradiation with blue light-emitting diode enhances osteogenic differentiation of stem cells from the apical papilla. Lasers Med Sci 35, 1981–1988 (2020). https://doi.org/10.1007/s10103-020-02995-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10103-020-02995-3

Keywords

Search

Navigation