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Photobiostimulation activity of different low-level laser dosage on masticatory muscles and temporomandibular joint in an induced arthritis rat model

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Abstract

This study aimed to investigate the anti-inflammatory effects of different dosage of low-level laser therapy (LLLT) in an experimental model of temporomandibular joint (TMJ) arthritis. One hundred male Wistar rats were used and divided into the following groups: CG, control group; AG, animals group with left TMJ arthritis induced by intra-articular injection of Complete Freund’s adjuvant - CFA; LG5, LG10 and LG20 - animals with arthritis and treated with LLLT at doses 5, 10, and 20 J/cm2, respectively. Morphological analysis was performed by TMJ histological sections stained with hematoxylin-eosin (HE), picrosirius (PSR), and toluidine blue (TB), as well as histomorphometric evaluation of cartilage, articular disc, and masticatory muscles. The amount of feed consumed within 3 weeks was evaluated, and biochemical analysis of TMJ tissues included measurement of sulfated glycosaminoglycans (GAGs), matrix metalloproteinases (MMPs) 2 and 9 zymography, and ELISA for cytokines IL-6, TNF-α, and IL-1β. Only the 20 J/cm2 dose promoted higher feed intake compared to AG. On the other hand, all LLLT doses promoted better organization of articular disc collagen fibers, greater number of proteoglycans in articular cartilage, increased area and diameter of left lateral pterygoid fibers, reduced latent and active MMP 9 and 2 activity, and lower IL-1β concentration compared to AG. Considering the study limitations, it was observed that LLLT treatments were effective in protecting and tissue cleansing joint structures, accelerating tissue repair, especially at lower doses.

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Authors and Affiliations

  1. Institute of Biological Sciences and Health, Federal University of Alagoas – UFAL, Lourival Melo Mota, s/n, Tabuleiro dos Martins, Maceió, AL, 57072-900, Brazil

    George Azevedo Lemos

  2. Department of Restorative Dentistry, Federal University of Paraíba – UFPB, João Pessoa, PB, Brazil

    André Ulisses Dantas Batista

  3. Nursing and Medical Schools Nova Esperança (FAMENE/FACENE), João Pessoa, PB, Brazil

    Pâmela Lopes Pedro da Silva

  4. Unifacisa University Center, Campina Grande, PB, Brazil

    Diego Neves Araújo & Wendra Emmanuelly Abrantes Sarmento

  5. Department of Structural and Functional Biology, Institute of Biology, University of Campinas – UNICAMP, Campinas, SP, Brazil

    Evanisi Teresa Palomari

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  1. George Azevedo Lemos
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Correspondence to George Azevedo Lemos.

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Lemos, G.A., Batista, A.U.D., da Silva, P.L.P. et al. Photobiostimulation activity of different low-level laser dosage on masticatory muscles and temporomandibular joint in an induced arthritis rat model. Lasers Med Sci 35, 1129–1139 (2020). https://doi.org/10.1007/s10103-019-02933-y

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