Abstract
Deep tissue imaging using two-photon fluorescence (TPF) techniques have revolutionized the optical imaging community by providing in depth molecular information at the single-cell level. These techniques provide structural and functional aspects of mammalian brain at unprecedented depth and resolution. However, wavefront distortions introduced by the optical system as well as the biological sample (tissue) limit the achievable fluorescence signal-to-noise ratio and resolution with penetration depth. In this review, we discuss on the advances in TPF microscopy techniques for in vivo functional imaging and offer guidelines as to which technologies are best suited for different imaging applications with special reference to adaptive optics.
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Acknowledgements
We thank Dr. K. Satyamoorthy, Director, Manipal School of Life Sciences (MSLS), Manipal for his encouragement. Authors thank Dr. K. K. Mahato, Head of Department of Biophysics, MSLS for his constant support and Manipal Academy of Higher Education (MAHE), Manipal, India, for providing the infrastructure needed.
Funding
We reeived financial support from the SERB-Department of Science and Technology (DST), Government of India (Project Number—ECR/2016/001944).
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Sahu, P., Mazumder, N. Advances in adaptive optics–based two-photon fluorescence microscopy for brain imaging. Lasers Med Sci 35, 317–328 (2020). https://doi.org/10.1007/s10103-019-02908-z
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DOI: https://doi.org/10.1007/s10103-019-02908-z