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Effects of low-level laser irradiation on proliferation and functional protein expression in human RPE cells

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Abstract

Low-level laser irradiation (LLLI) modulates a set of biological effects in many cell types such as fibroblasts, keratinocytes, and stem cells. However, no study to date has reported the effects of LLLI on retinal pigment epithelia (RPE) cells. The aim of this study was to investigate whether LLLI could enhance the proliferation of RPE cells and increase the expression of RPE functional genes/proteins. Human ARPE-19 cells were seeded overnight and treated with 8 J/cm2 of LLLI. Cell proliferation was measured by CCK8 assay and cell cycle distribution was evaluated by FACS. The transcription of cell cycle-specific genes and RPE functional genes was quantified by RT-PCR. Moreover, the expression of ZO-1 and CRALBP were evaluated by immunostaining. A dose of 8 J/cm2 of LLLI significantly increased proliferation and promoted cell cycle progression while upregulating the transcription of CDK4 and CCND1 and decreasing the transcription of CDKN2A, CDKN2C, and CDKN1B in human ARPE-19 cells. Additionally, LLLI enhanced the expression of ZO-1 and CRALBP in human ARPE-19 cells. In conclusion, LLLI could enhance the proliferative ability of human ARPE-19 cells by modulating cyclin D1, CDK4, and a group of cyclin-dependent kinase inhibitors. It also could increase the expression of RPE-specific proteins. Thus, LLLI may be a potential approach for the treatment of RPE degenerative diseases.

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (no. 81371017), the International Cooperation Foundation of Henan Province (no. 2015GH12), and the International Training Project for Excellent Scholars of Henan Province 2015 (2015-13).

Conflict of interest

The authors have declared no conflicts of interest.

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Author notes

    Authors and Affiliations

    1. Department of Ophthalmology, The First Affiliated Hospital of Zhengzhou University, 1 East Jianshe Road, Erqi District, Zhengzhou, China

      Yalong Dang & Yu Zhu

    2. Clinical Stem Cell Research Center, Peking University Third Hospital, 49 Huayuan North Road, Haidian District, Beijing, China

      Yalong Dang, Wentao Wu, Yongsheng Xu, Haotian Wu & Chun Zhang

    3. Clinical Lab of Tissue & Cell Research Center, Department of Biotech Treatment, Logistics College of Chinese People’s Armed Police Force, Tianjin, China

      Yongsheng Xu

    4. Department of Ophthalmology, Yellow-River Hospital, Henan University of Science and Technology, Sanmenxia, China

      Yalin Mu & Ke Xu

    5. Beijing No.4 High School, Beijing, China

      Haotian Wu

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    1. Yalong Dang
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    Correspondence to Yu Zhu or Chun Zhang.

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    Yalong Dang, Wentao Wu and Yongsheng Xu contributed equally to this work.

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    Dang, Y., Wu, W., Xu, Y. et al. Effects of low-level laser irradiation on proliferation and functional protein expression in human RPE cells. Lasers Med Sci 30, 2295–2302 (2015). https://doi.org/10.1007/s10103-015-1809-3

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    • DOI: https://doi.org/10.1007/s10103-015-1809-3

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