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Evaluation of RIDASCREEN® and RIDA®QUICK Helicobacter kits for Helicobacter pylori detection in stools

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European Journal of Clinical Microbiology & Infectious Diseases Aims and scope Submit manuscript

Abstract

The diagnosis of Helicobacter pylori infection can be made by using noninvasive tests. The detection of bacterial antigens in stool samples is a technique proposed by some suppliers. The objective of this study was to evaluate retrospectively the performances of the commercially available RIDA®QUICK Helicobacter and RIDASCREEN® Helicobacter (R-Biopharm) kits in detecting H. pylori antigens in stool samples. A collection of 132 stools was used in this study: 94 stools obtained from H. pylori-negative patients and 38 stools from H. pylori-positive patients. The performances (sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV)) were evaluated for the RIDA®QUICK Helicobacter and RIDASCREEN® Helicobacter kits in comparison with real-time PCR results performed on gastric biopsies as well as culture. Discordant results, with respect to H. pylori status, were checked on the same day as the test by repeating the procedure. All of the readings concerning the RIDA®QUICK Helicobacter tests were concordant between 3 users, i.e., 94/94 negative tests and 34/38 positive tests. RIDASCREEN® Helicobacter tests were negative for all 94 H. pylori-negative samples and positive for 35/38 positive stools. Reading of the RIDA®QUICK Helicobacter tests was not a problem in routine practice. The RIDA®QUICK Helicobacter and RIDASCREEN® Helicobacter kits show good performances and can be included in the armamentarium of diagnostic tests for H. pylori infection.

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Acknowledgments

The authors want to thank all of the clinicians and laboratories who sent samples to our reference center for H. pylori diagnosis. We also thank Mrs. Lindsay Mégraud, a native English speaker, who carefully reviewed this paper.

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Authors and Affiliations

  1. French National Reference Centre for Campylobacters & Helicobacters, Hôpital Pellegrin, 33076, Bordeaux, France

    Lucie Bénéjat, Alice Buissonnière, Astrid Ducournau, Francis Mégraud, Emilie Bessède & Philippe Lehours

  2. INSERM, UMR1053 Bordeaux BaRITOn, University of Bordeaux, 33076, Bordeaux, France

    Francis Mégraud, Emilie Bessède & Philippe Lehours

Authors
  1. Lucie Bénéjat
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  2. Alice Buissonnière
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  3. Astrid Ducournau
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  4. Francis Mégraud
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  5. Emilie Bessède
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  6. Philippe Lehours
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Contributions

PL supervised the study. FM, EB, and PL analyzed the data and drafted the paper. LB, AB, and AD performed the experiments. All authors interpreted the data. All authors critically revised the manuscript for important intellectual content.

Corresponding author

Correspondence to Philippe Lehours.

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Conflict of interest

The authors declare no conflict of interest. R-Biopharm (Darmstadt, Germany) which commercializes the RIDA®QUICK Helicobacter and RIDASCREEN® Helicobacter tests in France provided the kits but was not involved in the study design or in the data analysis.

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Bénéjat, L., Buissonnière, A., Ducournau, A. et al. Evaluation of RIDASCREEN® and RIDA®QUICK Helicobacter kits for Helicobacter pylori detection in stools. Eur J Clin Microbiol Infect Dis 39, 1941–1943 (2020). https://doi.org/10.1007/s10096-020-03943-3

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  • DOI: https://doi.org/10.1007/s10096-020-03943-3

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