Abstract
OXA-370 is a recently described OXA-48 variant that has only been described in a few Enterobacter spp. and Klebsiella pneumoniae isolates. The purpose of this study is to assess the prevalence of OXA-370-producing isolates in carbapenem-nonsusceptible Enterobacteriaceae recovered from 28 hospitals from Brazil. Real-time PCR was used to determine the presence of bla NDM-1, bla KPC-2, bla VIM-type, bla GES-type, bla OXA-48-like, and bla IMP-type genes. A total of 4,451 Enterobacteriaceae were screened. The gene bla OXA-48-like was detected in 74 (2.5%) isolates, mostly of Enterobacter spp. (44.6% E. cloacae and 2.7% E. aerogenes) and Klebsiella spp. (31.1% K. pneumoniae and 6.7% K. oxytoca), followed by Escherichia coli, (6.7%), Morganella morganii, (2.7%), Citrobacter freundii (1.3%), Proteus mirabilis (1.3%), Providencia stuartii (1.3%), and Serratia spp. (1.3%). These isolates were from five hospitals, 67 (90.5%) from the hospital where the bla OXA-370 was first described. Sequencing of bla OXA-48-like was performed in 52 isolates, including E. cloacae, E. aerogenes, K. pneumoniae, K. oxytoca, E. coli, and C. freundii; all presenting 100% identity with bla OXA-370. PFGE revealed the presence of distinct clones among K. pneumoniae, E. cloacae, K. oxytoca, and E. coli. Susceptibility rates to meropenem, imipenem, and ertapenem among OXA-370-producing isolates were 92.3%, 78.8%, 7.7% respectively; the MIC50 /MIC90 were 0.38/2 mg/L and 1/3 mg/L for meropenem and imipenem respectively. Overall, antimicrobial susceptibility analysis suggests that OXA-370 lacks carbapenemase activity. Our study demonstrated that the bla OXA-370 gene is disseminated among several Enterobacteriaceae species and clones, indicating a high potential for dissemination.
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This work was supported by the Fundo de Incentivo à Pesquisa e Eventos do Hospital de Clínicas de Porto Alegre, Coordenacão de Aperfeiçoamento de Pessoal de Nível Superior, Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, and the National Council for Scientific and Technological Development (CNPq), Ministry of Science and Technology, Brazil.
Conflicts of interest
A. P.Z. has received honoraria for speaking engagements and consultancy from Merck, AstraZeneca, Pfizer, and United Pharmaceuticals. None of the other authors has any conflicts of interest to declare.
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The study was approved by the Ethics Committee of the Hospital de Clínicas de Porto Alegre (14–0046).
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Magagnin, C.M., Rozales, F.P., Antochevis, L. et al. Dissemination of bla OXA-370 gene among several Enterobacteriaceae species in Brazil. Eur J Clin Microbiol Infect Dis 36, 1907–1910 (2017). https://doi.org/10.1007/s10096-017-3012-x
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DOI: https://doi.org/10.1007/s10096-017-3012-x