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Sequence variation in virulence-related genes of Bordetella pertussis isolates from Poland in the period 1959–2013

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Abstract

This study aimed to characterise Bordetella pertussis isolates circulating in Poland since 1959. Sequence analysis of ptxA, ptxC, prn, tcfA, fim2, fim3 and ptxP for 175 clinical isolates and currently and previously used vaccine strains was performed. Clinical isolates from the period 1995–2013 were found to be different to three currently used vaccine strains harbouring the allelic combination ptxA2–ptxC1–ptxP1–prn1–tcfA2–fim2-1–fim3-1, seen frequently in Poland in the early pertussis vaccination period but not found after 1995. Generally, among B. pertussis isolates from the period 2000–2013, two genotypes predominated, ptxA1–ptxC1–ptxP1–prn1–tcfA2–fim2-2–fim3-1 and ptxA1–ptxC1–ptxP1–prn2–tcfA2–fim2-1–fim3-1, with frequencies of 45 % and 32.5 %, respectively. The isolates harbouring ptxA1–ptxC2–ptxP3–prn2–tcfA2–fim2-1–fim3-2 and ptxA1–ptxC2–ptxP3–prn2–tcfA2–fim2-1–fim3-1 profiles, currently highly prevalent within other European Union (EU) countries, were rarely found in Poland, as they circulated in the period 2000–2013 with frequencies of 10 % and 5 %, respectively. We hypothesise that several previous changes of strain composition in whole-cell pertussis vaccine produced locally and used since 1960 in Poland resulted in a more diverse immune pressure in the population, resulting in different prevalence of alleles compared to elsewhere.

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Acknowledgements

We thank Marjolein van Gent and Frits Mooi (RVIM, The Netherlands) for their help in the assignment of novel ptxA and ptxC alleles.

Funding

The sequencing of ptxA, ptxC, prn, tcfA, fim2, fim3 and ptxP were supported by the Ministry of Education and Science in Poland (grant number N N404 206 539) and the National Science Centre (grant number 2011/01/N/NZ7/05305).

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Sera and Vaccines Evaluation, National Institute of Public Health—National Institute of Hygiene, Warsaw, Poland

    E. Mosiej, M. Zawadka, K. Krysztopa-Grzybowska, M. Polak, E. Augustynowicz & A. Lutyńska

  2. Department of Bacteriology, National Institute of Public Health—National Institute of Hygiene, Warsaw, Poland

    K. Piekarska

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  1. E. Mosiej
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Correspondence to A. Lutyńska.

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Mosiej, E., Zawadka, M., Krysztopa-Grzybowska, K. et al. Sequence variation in virulence-related genes of Bordetella pertussis isolates from Poland in the period 1959–2013. Eur J Clin Microbiol Infect Dis 34, 147–152 (2015). https://doi.org/10.1007/s10096-014-2216-6

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  • DOI: https://doi.org/10.1007/s10096-014-2216-6

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