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Anwulignan alleviates d-galactose induced renal damage by regulating Nrf2/ARE signaling pathway in mice

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Abstract

Free radical accumulation in the body will cause oxidative stress damages including the renal damage. Schisandrae Sphenantherae Fructus (Schisandra), a traditional Chinese herbal medicine, has been used throughout the world. Anwulignan, a monomer extracted from Schisandra, has been shown in our previous studies to possess antioxidant and protective effects on the liver, brain and spleen damages in the aging mice. However, its effect on the renal damage caused by aging is not clear. This study showed that anwulignan could significantly increase the kidney index, the creatinine clearance, the activities of superoxide dismutase, catalase and glutathione peroxidase; reduce the urinary protein concentration, the serum urea nitrogen and creatinine content, the content of malondialdehyde and 8-hydroxylated deoxyguanosine in the renal tissue; and improve the renal tissue damage. Moreover, anwulignan increased the production of Nrf2, HO-1 and NQO1 proteins and decreased the production level of Keap1 protein in the renal tissue in the d-galactose induced aging mice. These results suggest that anwulignan significantly alleviates the renal damage by its antioxidant effect through regulating the production of Nrf2/ARE pathway-related proteins in the renal tissue in the d-galactose induced aging mice.

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Acknowledgements

The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.This research was funded by Jilin Provincial Department of science and Technology (Grant Nos. 20170309006YY, 20200201521JC, 20200404053YY and 20200404022YY), Jilin Science and technology innovation development plan project (Grant No. 20190601177), Jilin provincial health and Family Planning Commission (Grant No. 2018J089, 2019J046), Jilin Provincial Development and Reform Commission (Grant No. 2020C033-2) and Jilin Administration of traditional Chinese Medicine (Grant No. 2020121).

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Authors and Affiliations

  1. Department of Pharmacology, College of Pharmacy, Beihua University, Jilin, China

    Chunna Liu, Huijiao Lin, Liu Jiawei, Yao Wang, Chunmei Wang, Jinghui Sun, Jianguang Chen & He Li

  2. Department of Pathology, Medical College, Beihua University, Jilin, 132013, China

    Chunyan Yu & Ying Dong

  3. Department of Molecular Biology Test Technique, College of Medical Technology, Beihua University, Jilin, China

    Wenyue Zhuang

  4. Department of General Surgery, Affiliated Hospital of Beihua University, Jilin, Jilin, China

    Shu Jing

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  1. Chunna Liu
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Correspondence to He Li.

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Liu, C., Lin, H., Jiawei, L. et al. Anwulignan alleviates d-galactose induced renal damage by regulating Nrf2/ARE signaling pathway in mice. Food Sci Biotechnol 30, 1097–1105 (2021). https://doi.org/10.1007/s10068-021-00951-7

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