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Effect of controlled differential sieving processing on micronutrient contents and in vivo antioxidant activities of Hibiscus sabdariffa L. calyxes powder

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Abstract

Sun-dried calyxes of Hibiscus sabdariffa were finely grinded and fractionated by controlled differential sieving processing (CDSp) into four granulometric classes. The obtained powder fractions were examined for their micronutrient contents and in vivo antioxidant properties in high-fat diet induced oxidation model rats. Unsieved powder and lyophilized ethanolic extract were used for comparison. Micronutrient contents and antioxidant properties were significantly influenced (p < 0.05) by particle sizes. The mineral, vitamin, carotenoid and anthocyanin contents were shown to be higher in smaller powder particles. Comparatively, the ethanolic extract powder had the higher carotenoid, vitamin and anthocyanin contents while the superfine powders < 180 µm and 180–212 µm had the higher mineral contents and antioxidant properties which was characterized by high superoxide dismutase, catalase activities and low malondialdehyde production. Plant grinding followed by CDSp may be an option to improve micronutrient content and antioxidant activity of plant powders.

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Acknowledgements

The work was supported by the Francophone University Association (AUF) and its grant for Scientific Research, No. S0020ADM10607L. The work was carried out within the frame of the Extrapole project funded by the former Lorraine region (France). The authors are grateful to the staff of LABBAN (Laboratory of Biophysics, Food Biochemistry and Nutrition of Ngaoundere University, Cameroon) as well as at the LIBio (Biomolecular Engineering Laboratory, University of Lorraine, France) for their technical support.

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Authors and Affiliations

  1. Department of Food Sciences and Nutrition, ENSAI, University of Ngaoundere, PO Box 455, Ngaoundere, Cameroon

    Markusse Deli, Richard Marcel Nguimbou, Elie Njantou Baudelaire, Nicolas Njintang Yanou & Carl Moses Mbofung

  2. Laboratoire d’Ingénierie des Biomolécules (LIBio), Université de Lorraine, 2, Avenue de la Forêt de Haye, B.P. 172, 54500, Vandœuvre-lès-Nancy, France

    Elie Njantou Baudelaire & Joël Scher

  3. Department of Biological Sciences, Faculty of Sciences, University of Ngaoundere, PO Box 454, Ngaoundere, Cameroon

    Nicolas Njintang Yanou

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  1. Markusse Deli
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Correspondence to Richard Marcel Nguimbou.

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Research approval was obtained from the Cameroonian National Ethics Committee Ref. No. FWIRD00001954.

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Deli, M., Nguimbou, R.M., Njantou Baudelaire, E. et al. Effect of controlled differential sieving processing on micronutrient contents and in vivo antioxidant activities of Hibiscus sabdariffa L. calyxes powder. Food Sci Biotechnol 29, 1741–1753 (2020). https://doi.org/10.1007/s10068-020-00828-1

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